Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1532 and Pf1N1B4_1533 are separated by 246 nucleotides Pf1N1B4_1533 and Pf1N1B4_1534 are separated by 124 nucleotides
Pf1N1B4_1532 - Uroporphyrinogen III decarboxylase (EC 4.1.1.37), at 1,763,850 to 1,764,917
_1532
Pf1N1B4_1533 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 1,765,164 to 1,766,582
_1533
Pf1N1B4_1534 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 1,766,707 to 1,771,086
_1534
Position (kb)
1765
1766
1767 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1765.160 kb on - strand at 1765.160 kb on - strand at 1765.177 kb on + strand at 1765.178 kb on - strand at 1765.295 kb on - strand at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.664 kb on + strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.665 kb on - strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.843 kb on + strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.844 kb on - strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.849 kb on + strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.850 kb on - strand at 1765.850 kb on - strand, within Pf1N1B4_1533 at 1765.879 kb on + strand, within Pf1N1B4_1533 at 1765.879 kb on + strand, within Pf1N1B4_1533 at 1766.200 kb on + strand, within Pf1N1B4_1533 at 1766.200 kb on + strand, within Pf1N1B4_1533 at 1766.200 kb on + strand, within Pf1N1B4_1533 at 1766.200 kb on + strand, within Pf1N1B4_1533 at 1766.201 kb on - strand, within Pf1N1B4_1533 at 1766.201 kb on - strand, within Pf1N1B4_1533 at 1766.201 kb on - strand, within Pf1N1B4_1533 at 1766.201 kb on - strand, within Pf1N1B4_1533 at 1766.201 kb on - strand, within Pf1N1B4_1533 at 1766.201 kb on - strand, within Pf1N1B4_1533 at 1766.479 kb on + strand at 1766.480 kb on - strand at 1766.480 kb on - strand at 1766.480 kb on - strand at 1766.586 kb on + strand at 1766.587 kb on - strand at 1766.587 kb on - strand at 1766.587 kb on - strand at 1766.587 kb on - strand at 1766.681 kb on - strand at 1766.681 kb on - strand at 1766.728 kb on - strand at 1766.728 kb on - strand at 1766.728 kb on - strand at 1766.761 kb on - strand at 1766.789 kb on + strand at 1766.789 kb on + strand at 1766.789 kb on + strand at 1766.790 kb on - strand at 1766.846 kb on + strand at 1766.846 kb on + strand at 1766.846 kb on + strand at 1766.847 kb on - strand at 1766.847 kb on - strand at 1766.847 kb on - strand at 1767.005 kb on + strand at 1767.005 kb on + strand at 1767.006 kb on - strand at 1767.022 kb on + strand at 1767.023 kb on - strand at 1767.047 kb on + strand at 1767.047 kb on + strand at 1767.048 kb on - strand at 1767.048 kb on - strand at 1767.048 kb on - strand at 1767.099 kb on + strand at 1767.099 kb on + strand at 1767.099 kb on + strand at 1767.099 kb on + strand at 1767.203 kb on + strand, within Pf1N1B4_1534 at 1767.203 kb on + strand, within Pf1N1B4_1534 at 1767.203 kb on + strand, within Pf1N1B4_1534 at 1767.203 kb on + strand, within Pf1N1B4_1534 at 1767.203 kb on + strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.204 kb on - strand, within Pf1N1B4_1534 at 1767.207 kb on + strand, within Pf1N1B4_1534 at 1767.207 kb on + strand, within Pf1N1B4_1534 at 1767.207 kb on + strand, within Pf1N1B4_1534 at 1767.208 kb on - strand, within Pf1N1B4_1534 at 1767.208 kb on - strand, within Pf1N1B4_1534 at 1767.208 kb on - strand, within Pf1N1B4_1534 at 1767.263 kb on + strand, within Pf1N1B4_1534 at 1767.263 kb on + strand, within Pf1N1B4_1534 at 1767.264 kb on - strand, within Pf1N1B4_1534 at 1767.264 kb on - strand, within Pf1N1B4_1534 at 1767.264 kb on - strand, within Pf1N1B4_1534 at 1767.264 kb on - strand, within Pf1N1B4_1534 at 1767.287 kb on + strand, within Pf1N1B4_1534 at 1767.287 kb on + strand, within Pf1N1B4_1534 at 1767.287 kb on + strand at 1767.287 kb on + strand, within Pf1N1B4_1534 at 1767.287 kb on + strand, within Pf1N1B4_1534 at 1767.287 kb on + strand, within Pf1N1B4_1534 at 1767.287 kb on + strand at 1767.287 kb on + strand, within Pf1N1B4_1534 at 1767.288 kb on - strand, within Pf1N1B4_1534 at 1767.288 kb on - strand, within Pf1N1B4_1534 at 1767.288 kb on - strand, within Pf1N1B4_1534 at 1767.288 kb on - strand, within Pf1N1B4_1534 at 1767.288 kb on - strand, within Pf1N1B4_1534
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 1,765,160 - +0.3 1,765,160 - +0.1 1,765,177 + -1.0 1,765,178 - -4.3 1,765,295 - +0.0 1,765,664 + Pf1N1B4_1533 0.35 -0.4 1,765,664 + Pf1N1B4_1533 0.35 -1.2 1,765,664 + Pf1N1B4_1533 0.35 -1.4 1,765,664 + Pf1N1B4_1533 0.35 -1.4 1,765,664 + Pf1N1B4_1533 0.35 -3.1 1,765,664 + Pf1N1B4_1533 0.35 -0.4 1,765,665 - Pf1N1B4_1533 0.35 -1.2 1,765,665 - Pf1N1B4_1533 0.35 -0.4 1,765,665 - Pf1N1B4_1533 0.35 -1.4 1,765,665 - Pf1N1B4_1533 0.35 -1.1 1,765,665 - Pf1N1B4_1533 0.35 -0.7 1,765,843 + Pf1N1B4_1533 0.48 -2.1 1,765,843 + Pf1N1B4_1533 0.48 -2.6 1,765,843 + Pf1N1B4_1533 0.48 -1.4 1,765,843 + Pf1N1B4_1533 0.48 -2.6 1,765,843 + Pf1N1B4_1533 0.48 -2.5 1,765,843 + Pf1N1B4_1533 0.48 -0.7 1,765,843 + Pf1N1B4_1533 0.48 -1.0 1,765,843 + Pf1N1B4_1533 0.48 -0.5 1,765,844 - Pf1N1B4_1533 0.48 -0.7 1,765,844 - Pf1N1B4_1533 0.48 +0.0 1,765,844 - Pf1N1B4_1533 0.48 -0.6 1,765,844 - Pf1N1B4_1533 0.48 -2.0 1,765,844 - Pf1N1B4_1533 0.48 -1.8 1,765,844 - Pf1N1B4_1533 0.48 -4.5 1,765,844 - Pf1N1B4_1533 0.48 -1.8 1,765,844 - Pf1N1B4_1533 0.48 -2.2 1,765,844 - Pf1N1B4_1533 0.48 -0.7 1,765,844 - Pf1N1B4_1533 0.48 -1.6 1,765,844 - Pf1N1B4_1533 0.48 -2.8 1,765,844 - Pf1N1B4_1533 0.48 -3.6 1,765,849 + Pf1N1B4_1533 0.48 -2.0 1,765,849 + Pf1N1B4_1533 0.48 -1.4 1,765,849 + Pf1N1B4_1533 0.48 -0.6 1,765,849 + Pf1N1B4_1533 0.48 -1.6 1,765,849 + Pf1N1B4_1533 0.48 +0.0 1,765,849 + Pf1N1B4_1533 0.48 -3.3 1,765,849 + Pf1N1B4_1533 0.48 -0.2 1,765,849 + Pf1N1B4_1533 0.48 -2.2 1,765,849 + Pf1N1B4_1533 0.48 -0.7 1,765,849 + Pf1N1B4_1533 0.48 -0.4 1,765,849 + Pf1N1B4_1533 0.48 -1.0 1,765,849 + Pf1N1B4_1533 0.48 -2.8 1,765,850 - Pf1N1B4_1533 0.48 -0.4 1,765,850 - Pf1N1B4_1533 0.48 -1.6 1,765,850 - Pf1N1B4_1533 0.48 -1.4 1,765,850 - Pf1N1B4_1533 0.48 +0.0 1,765,850 - Pf1N1B4_1533 0.48 -2.2 1,765,850 - Pf1N1B4_1533 0.48 -0.4 1,765,850 - +0.0 1,765,850 - Pf1N1B4_1533 0.48 -2.2 1,765,879 + Pf1N1B4_1533 0.50 -1.6 1,765,879 + Pf1N1B4_1533 0.50 -1.7 1,766,200 + Pf1N1B4_1533 0.73 +0.6 1,766,200 + Pf1N1B4_1533 0.73 -0.7 1,766,200 + Pf1N1B4_1533 0.73 -3.1 1,766,200 + Pf1N1B4_1533 0.73 -1.5 1,766,201 - Pf1N1B4_1533 0.73 -1.8 1,766,201 - Pf1N1B4_1533 0.73 -1.7 1,766,201 - Pf1N1B4_1533 0.73 +0.0 1,766,201 - Pf1N1B4_1533 0.73 -0.4 1,766,201 - Pf1N1B4_1533 0.73 -1.2 1,766,201 - Pf1N1B4_1533 0.73 -1.7 1,766,479 + -1.7 1,766,480 - -1.0 1,766,480 - -0.4 1,766,480 - -2.3 1,766,586 + -3.2 1,766,587 - -0.4 1,766,587 - -2.0 1,766,587 - -2.0 1,766,587 - -0.4 1,766,681 - -0.4 1,766,681 - +0.6 1,766,728 - -1.6 1,766,728 - -1.6 1,766,728 - -0.3 1,766,761 - -0.4 1,766,789 + -2.0 1,766,789 + -2.5 1,766,789 + -1.7 1,766,790 - -2.5 1,766,846 + +0.0 1,766,846 + -2.1 1,766,846 + -1.8 1,766,847 - -2.3 1,766,847 - -1.8 1,766,847 - -0.6 1,767,005 + +0.2 1,767,005 + -0.3 1,767,006 - -1.0 1,767,022 + -2.1 1,767,023 - -2.2 1,767,047 + -2.8 1,767,047 + -1.4 1,767,048 - -0.7 1,767,048 - +1.6 1,767,048 - -0.7 1,767,099 + -2.0 1,767,099 + -3.0 1,767,099 + -3.1 1,767,099 + -2.7 1,767,203 + Pf1N1B4_1534 0.11 -2.8 1,767,203 + Pf1N1B4_1534 0.11 -1.7 1,767,203 + Pf1N1B4_1534 0.11 -3.7 1,767,203 + Pf1N1B4_1534 0.11 -2.1 1,767,203 + Pf1N1B4_1534 0.11 -2.2 1,767,204 - Pf1N1B4_1534 0.11 -2.1 1,767,204 - Pf1N1B4_1534 0.11 -0.7 1,767,204 - Pf1N1B4_1534 0.11 -2.3 1,767,204 - Pf1N1B4_1534 0.11 -1.7 1,767,204 - Pf1N1B4_1534 0.11 -1.7 1,767,204 - Pf1N1B4_1534 0.11 -2.2 1,767,204 - Pf1N1B4_1534 0.11 -1.6 1,767,204 - Pf1N1B4_1534 0.11 -1.8 1,767,204 - Pf1N1B4_1534 0.11 -1.0 1,767,204 - Pf1N1B4_1534 0.11 -3.0 1,767,204 - Pf1N1B4_1534 0.11 -2.4 1,767,204 - Pf1N1B4_1534 0.11 -3.4 1,767,204 - Pf1N1B4_1534 0.11 -3.0 1,767,204 - Pf1N1B4_1534 0.11 -0.7 1,767,204 - Pf1N1B4_1534 0.11 -3.0 1,767,207 + Pf1N1B4_1534 0.11 -2.8 1,767,207 + Pf1N1B4_1534 0.11 -1.0 1,767,207 + Pf1N1B4_1534 0.11 -1.8 1,767,208 - Pf1N1B4_1534 0.11 -2.6 1,767,208 - Pf1N1B4_1534 0.11 -1.4 1,767,208 - Pf1N1B4_1534 0.11 -2.5 1,767,263 + Pf1N1B4_1534 0.13 -2.4 1,767,263 + Pf1N1B4_1534 0.13 -1.7 1,767,264 - Pf1N1B4_1534 0.13 -3.6 1,767,264 - Pf1N1B4_1534 0.13 -1.2 1,767,264 - Pf1N1B4_1534 0.13 -4.0 1,767,264 - Pf1N1B4_1534 0.13 -2.1 1,767,287 + Pf1N1B4_1534 0.13 +0.0 1,767,287 + Pf1N1B4_1534 0.13 -2.0 1,767,287 + +1.6 1,767,287 + Pf1N1B4_1534 0.13 -2.6 1,767,287 + Pf1N1B4_1534 0.13 -0.4 1,767,287 + Pf1N1B4_1534 0.13 -0.7 1,767,287 + +0.6 1,767,287 + Pf1N1B4_1534 0.13 -4.1 1,767,288 - Pf1N1B4_1534 0.13 -2.1 1,767,288 - Pf1N1B4_1534 0.13 -0.7 1,767,288 - Pf1N1B4_1534 0.13 -1.8 1,767,288 - Pf1N1B4_1534 0.13 -1.6 1,767,288 - Pf1N1B4_1534 0.13 -2.3
Or see this region's nucleotide sequence