Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_13 and Pf1N1B4_14 are separated by 152 nucleotides Pf1N1B4_14 and Pf1N1B4_15 are separated by 11 nucleotides Pf1N1B4_15 and Pf1N1B4_16 are separated by 158 nucleotides
Pf1N1B4_13 - SAM-dependent methyltransferase YafE (UbiE paralog), at 14,449 to 15,216
_13
Pf1N1B4_14 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 15,369 to 16,013
_14
Pf1N1B4_15 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 16,025 to 17,443
_15
Pf1N1B4_16 - Cys regulon transcriptional activator CysB, at 17,602 to 18,492
_16
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 15.152 kb on + strand at 15.152 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.185 kb on - strand at 15.187 kb on + strand at 15.679 kb on + strand, within Pf1N1B4_14 at 15.680 kb on - strand, within Pf1N1B4_14 at 15.680 kb on - strand, within Pf1N1B4_14 at 16.026 kb on + strand at 16.027 kb on - strand at 16.455 kb on + strand, within Pf1N1B4_15 at 16.455 kb on + strand, within Pf1N1B4_15 at 16.456 kb on - strand, within Pf1N1B4_15 at 16.456 kb on - strand, within Pf1N1B4_15 at 16.456 kb on - strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.774 kb on + strand, within Pf1N1B4_15 at 16.775 kb on - strand, within Pf1N1B4_15 at 17.165 kb on - strand, within Pf1N1B4_15 at 17.364 kb on + strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.471 kb on - strand at 17.823 kb on - strand, within Pf1N1B4_16 at 17.927 kb on + strand, within Pf1N1B4_16 at 17.931 kb on - strand, within Pf1N1B4_16 at 18.271 kb on + strand, within Pf1N1B4_16 at 18.272 kb on - strand at 18.392 kb on + strand, within Pf1N1B4_16 at 18.392 kb on + strand, within Pf1N1B4_16 at 18.392 kb on + strand, within Pf1N1B4_16 at 18.398 kb on - strand at 18.398 kb on - strand, within Pf1N1B4_16
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 15,152 + -2.3 15,152 + -1.3 15,184 + +0.1 15,184 + -0.3 15,184 + +1.6 15,185 - -0.4 15,187 + -1.6 15,679 + Pf1N1B4_14 0.48 -1.3 15,680 - Pf1N1B4_14 0.48 -1.6 15,680 - Pf1N1B4_14 0.48 -2.3 16,026 + -5.0 16,027 - +1.8 16,455 + Pf1N1B4_15 0.30 -1.3 16,455 + Pf1N1B4_15 0.30 -2.0 16,456 - Pf1N1B4_15 0.30 -3.1 16,456 - Pf1N1B4_15 0.30 -1.3 16,456 - Pf1N1B4_15 0.30 -2.6 16,704 + Pf1N1B4_15 0.48 +0.0 16,704 + Pf1N1B4_15 0.48 -2.3 16,704 + Pf1N1B4_15 0.48 -1.6 16,774 + Pf1N1B4_15 0.53 -1.0 16,775 - Pf1N1B4_15 0.53 -2.3 17,165 - Pf1N1B4_15 0.80 -3.8 17,364 + -1.0 17,365 - +0.0 17,365 - -2.6 17,365 - -1.0 17,365 - -1.0 17,365 - -1.3 17,471 - +0.6 17,823 - Pf1N1B4_16 0.25 -0.2 17,927 + Pf1N1B4_16 0.36 +0.4 17,931 - Pf1N1B4_16 0.37 +0.0 18,271 + Pf1N1B4_16 0.75 +1.0 18,272 - +2.0 18,392 + Pf1N1B4_16 0.89 +0.4 18,392 + Pf1N1B4_16 0.89 -0.2 18,392 + Pf1N1B4_16 0.89 -1.0 18,398 - +2.0 18,398 - Pf1N1B4_16 0.89 +0.5
Or see this region's nucleotide sequence