Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_15

Experiment: D-Gluconic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_13 and Pf1N1B4_14 are separated by 152 nucleotidesPf1N1B4_14 and Pf1N1B4_15 are separated by 11 nucleotidesPf1N1B4_15 and Pf1N1B4_16 are separated by 158 nucleotides Pf1N1B4_13 - SAM-dependent methyltransferase YafE (UbiE paralog), at 14,449 to 15,216 _13 Pf1N1B4_14 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 15,369 to 16,013 _14 Pf1N1B4_15 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 16,025 to 17,443 _15 Pf1N1B4_16 - Cys regulon transcriptional activator CysB, at 17,602 to 18,492 _16 Position (kb) 16 17 18Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 15.152 kb on + strandat 15.152 kb on + strandat 15.184 kb on + strandat 15.184 kb on + strandat 15.184 kb on + strandat 15.185 kb on - strandat 15.187 kb on + strandat 15.679 kb on + strand, within Pf1N1B4_14at 15.680 kb on - strand, within Pf1N1B4_14at 15.680 kb on - strand, within Pf1N1B4_14at 16.026 kb on + strandat 16.027 kb on - strandat 16.455 kb on + strand, within Pf1N1B4_15at 16.455 kb on + strand, within Pf1N1B4_15at 16.456 kb on - strand, within Pf1N1B4_15at 16.456 kb on - strand, within Pf1N1B4_15at 16.456 kb on - strand, within Pf1N1B4_15at 16.704 kb on + strand, within Pf1N1B4_15at 16.704 kb on + strand, within Pf1N1B4_15at 16.704 kb on + strand, within Pf1N1B4_15at 16.774 kb on + strand, within Pf1N1B4_15at 16.775 kb on - strand, within Pf1N1B4_15at 17.165 kb on - strand, within Pf1N1B4_15at 17.364 kb on + strandat 17.365 kb on - strandat 17.365 kb on - strandat 17.365 kb on - strandat 17.365 kb on - strandat 17.365 kb on - strandat 17.471 kb on - strandat 17.823 kb on - strand, within Pf1N1B4_16at 17.927 kb on + strand, within Pf1N1B4_16at 17.931 kb on - strand, within Pf1N1B4_16at 18.271 kb on + strand, within Pf1N1B4_16at 18.272 kb on - strandat 18.392 kb on + strand, within Pf1N1B4_16at 18.392 kb on + strand, within Pf1N1B4_16at 18.392 kb on + strand, within Pf1N1B4_16at 18.398 kb on - strandat 18.398 kb on - strand, within Pf1N1B4_16

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Gluconic Acid (C)
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15,152 + -2.3
15,152 + -1.3
15,184 + +0.1
15,184 + -0.3
15,184 + +1.6
15,185 - -0.4
15,187 + -1.6
15,679 + Pf1N1B4_14 0.48 -1.3
15,680 - Pf1N1B4_14 0.48 -1.6
15,680 - Pf1N1B4_14 0.48 -2.3
16,026 + -5.0
16,027 - +1.8
16,455 + Pf1N1B4_15 0.30 -1.3
16,455 + Pf1N1B4_15 0.30 -2.0
16,456 - Pf1N1B4_15 0.30 -3.1
16,456 - Pf1N1B4_15 0.30 -1.3
16,456 - Pf1N1B4_15 0.30 -2.6
16,704 + Pf1N1B4_15 0.48 +0.0
16,704 + Pf1N1B4_15 0.48 -2.3
16,704 + Pf1N1B4_15 0.48 -1.6
16,774 + Pf1N1B4_15 0.53 -1.0
16,775 - Pf1N1B4_15 0.53 -2.3
17,165 - Pf1N1B4_15 0.80 -3.8
17,364 + -1.0
17,365 - +0.0
17,365 - -2.6
17,365 - -1.0
17,365 - -1.0
17,365 - -1.3
17,471 - +0.6
17,823 - Pf1N1B4_16 0.25 -0.2
17,927 + Pf1N1B4_16 0.36 +0.4
17,931 - Pf1N1B4_16 0.37 +0.0
18,271 + Pf1N1B4_16 0.75 +1.0
18,272 - +2.0
18,392 + Pf1N1B4_16 0.89 +0.4
18,392 + Pf1N1B4_16 0.89 -0.2
18,392 + Pf1N1B4_16 0.89 -1.0
18,398 - +2.0
18,398 - Pf1N1B4_16 0.89 +0.5

Or see this region's nucleotide sequence