Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1474 and Pf1N1B4_1475 are separated by 216 nucleotides Pf1N1B4_1475 and Pf1N1B4_1476 overlap by 6 nucleotides
Pf1N1B4_1474 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 1,689,121 to 1,692,060
_1474
Pf1N1B4_1475 - FIG00962376: hypothetical protein, at 1,692,277 to 1,692,450
_1475
Pf1N1B4_1476 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 1,692,445 to 1,695,090
_1476
Position (kb)
1692
1693 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1691.603 kb on + strand, within Pf1N1B4_1474 at 1691.603 kb on + strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1692.026 kb on - strand at 1692.026 kb on - strand at 1692.066 kb on - strand at 1692.096 kb on + strand at 1692.096 kb on + strand at 1692.097 kb on - strand at 1692.097 kb on - strand at 1692.097 kb on - strand at 1692.228 kb on + strand at 1692.252 kb on + strand at 1692.275 kb on + strand at 1692.288 kb on + strand at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.348 kb on - strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.796 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 1,691,603 + Pf1N1B4_1474 0.84 -0.6 1,691,603 + Pf1N1B4_1474 0.84 +2.6 1,691,604 - Pf1N1B4_1474 0.84 +0.0 1,691,604 - Pf1N1B4_1474 0.84 -0.9 1,691,604 - Pf1N1B4_1474 0.84 -1.0 1,691,604 - Pf1N1B4_1474 0.84 +1.0 1,692,026 - +0.1 1,692,026 - +1.0 1,692,066 - -0.6 1,692,096 + -0.9 1,692,096 + -0.3 1,692,097 - +0.0 1,692,097 - -0.7 1,692,097 - -0.1 1,692,228 + +2.8 1,692,252 + +0.0 1,692,275 + -1.1 1,692,288 + +1.6 1,692,347 + Pf1N1B4_1475 0.40 +1.2 1,692,347 + Pf1N1B4_1475 0.40 -1.4 1,692,347 + Pf1N1B4_1475 0.40 -1.8 1,692,347 + Pf1N1B4_1475 0.40 -0.6 1,692,347 + Pf1N1B4_1475 0.40 -4.1 1,692,347 + Pf1N1B4_1475 0.40 -0.7 1,692,347 + Pf1N1B4_1475 0.40 -1.2 1,692,347 + Pf1N1B4_1475 0.40 -0.7 1,692,347 + Pf1N1B4_1475 0.40 +0.2 1,692,347 + Pf1N1B4_1475 0.40 +1.6 1,692,347 + Pf1N1B4_1475 0.40 +1.2 1,692,347 + Pf1N1B4_1475 0.40 +0.9 1,692,347 + Pf1N1B4_1475 0.40 -0.1 1,692,347 + Pf1N1B4_1475 0.40 -0.4 1,692,348 - Pf1N1B4_1475 0.41 +0.0 1,692,370 + Pf1N1B4_1475 0.53 +0.0 1,692,370 + Pf1N1B4_1475 0.53 -1.9 1,692,370 + Pf1N1B4_1475 0.53 -0.7 1,692,370 + Pf1N1B4_1475 0.53 +0.5 1,692,370 + Pf1N1B4_1475 0.53 -1.0 1,692,796 + +3.9
Or see this region's nucleotide sequence