Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1329

Experiment: D-Gluconic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1327 and Pf1N1B4_1328 overlap by 4 nucleotidesPf1N1B4_1328 and Pf1N1B4_1329 overlap by 8 nucleotidesPf1N1B4_1329 and Pf1N1B4_1330 are separated by 145 nucleotides Pf1N1B4_1327 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 1,522,428 to 1,523,162 _1327 Pf1N1B4_1328 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 1,523,159 to 1,523,785 _1328 Pf1N1B4_1329 - Cobalamin biosynthesis protein CobG, at 1,523,778 to 1,525,079 _1329 Pf1N1B4_1330 - hypothetical protein, at 1,525,225 to 1,525,881 _1330 Position (kb) 1523 1524 1525 1526Strain fitness (log2 ratio) -2 -1 0 1 2at 1522.778 kb on - strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strandat 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.804 kb on + strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.805 kb on - strandat 1522.805 kb on - strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.810 kb on + strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.811 kb on - strand, within Pf1N1B4_1327at 1522.819 kb on + strand, within Pf1N1B4_1327at 1522.820 kb on - strand, within Pf1N1B4_1327at 1522.903 kb on + strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.904 kb on - strand, within Pf1N1B4_1327at 1522.928 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.054 kb on - strand, within Pf1N1B4_1327at 1523.361 kb on + strandat 1523.361 kb on + strand, within Pf1N1B4_1328at 1523.361 kb on + strand, within Pf1N1B4_1328at 1523.361 kb on + strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.362 kb on - strand, within Pf1N1B4_1328at 1523.751 kb on + strandat 1523.751 kb on + strandat 1523.751 kb on + strandat 1523.751 kb on + strandat 1523.752 kb on - strandat 1523.752 kb on - strandat 1523.775 kb on + strandat 1523.775 kb on + strandat 1523.775 kb on + strandat 1523.775 kb on + strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.776 kb on - strandat 1523.834 kb on + strandat 1523.834 kb on + strandat 1523.834 kb on + strandat 1523.835 kb on - strandat 1523.835 kb on - strandat 1523.835 kb on - strandat 1523.835 kb on - strandat 1523.835 kb on - strandat 1523.837 kb on + strandat 1523.837 kb on + strandat 1523.838 kb on - strandat 1523.838 kb on - strandat 1523.838 kb on - strandat 1523.838 kb on - strandat 1523.838 kb on - strandat 1523.890 kb on + strandat 1523.891 kb on - strandat 1524.283 kb on - strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strandat 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.295 kb on + strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.296 kb on - strand, within Pf1N1B4_1329at 1524.301 kb on + strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.302 kb on - strand, within Pf1N1B4_1329at 1524.758 kb on + strand, within Pf1N1B4_1329at 1524.758 kb on + strand, within Pf1N1B4_1329at 1524.759 kb on - strand, within Pf1N1B4_1329at 1524.759 kb on - strand, within Pf1N1B4_1329at 1524.759 kb on - strand, within Pf1N1B4_1329at 1524.759 kb on - strand, within Pf1N1B4_1329at 1525.065 kb on + strandat 1525.066 kb on - strandat 1525.145 kb on - strandat 1525.201 kb on - strandat 1525.201 kb on - strandat 1525.208 kb on - strandat 1525.236 kb on - strandat 1525.377 kb on - strand, within Pf1N1B4_1330at 1525.377 kb on - strand, within Pf1N1B4_1330at 1525.377 kb on - strand, within Pf1N1B4_1330at 1525.383 kb on + strand, within Pf1N1B4_1330at 1525.384 kb on - strand, within Pf1N1B4_1330at 1525.448 kb on + strand, within Pf1N1B4_1330at 1525.464 kb on + strand, within Pf1N1B4_1330at 1525.729 kb on - strand, within Pf1N1B4_1330at 1525.731 kb on - strand, within Pf1N1B4_1330at 1525.731 kb on - strand, within Pf1N1B4_1330at 1525.759 kb on + strand, within Pf1N1B4_1330at 1525.825 kb on - strandat 1525.825 kb on - strandat 1525.825 kb on - strandat 1525.882 kb on - strandat 1525.975 kb on + strandat 1526.016 kb on + strandat 1526.016 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Gluconic Acid (C)
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1,522,778 - Pf1N1B4_1327 0.48 +0.1
1,522,804 + Pf1N1B4_1327 0.51 -0.1
1,522,804 + Pf1N1B4_1327 0.51 -1.7
1,522,804 + -0.1
1,522,804 + Pf1N1B4_1327 0.51 +0.5
1,522,804 + Pf1N1B4_1327 0.51 -0.7
1,522,804 + Pf1N1B4_1327 0.51 -1.0
1,522,805 - Pf1N1B4_1327 0.51 +0.3
1,522,805 - Pf1N1B4_1327 0.51 -0.1
1,522,805 - Pf1N1B4_1327 0.51 -0.8
1,522,805 - Pf1N1B4_1327 0.51 -0.8
1,522,805 - Pf1N1B4_1327 0.51 +0.3
1,522,805 - Pf1N1B4_1327 0.51 +0.7
1,522,805 - Pf1N1B4_1327 0.51 -1.0
1,522,805 - -0.8
1,522,805 - Pf1N1B4_1327 0.51 +0.5
1,522,810 + Pf1N1B4_1327 0.52 -0.6
1,522,810 + Pf1N1B4_1327 0.52 -0.5
1,522,810 + Pf1N1B4_1327 0.52 +0.5
1,522,810 + Pf1N1B4_1327 0.52 -0.7
1,522,810 + Pf1N1B4_1327 0.52 +0.7
1,522,810 + Pf1N1B4_1327 0.52 +0.3
1,522,810 + Pf1N1B4_1327 0.52 -0.0
1,522,810 + Pf1N1B4_1327 0.52 +0.1
1,522,810 + Pf1N1B4_1327 0.52 +0.0
1,522,810 + Pf1N1B4_1327 0.52 -0.7
1,522,810 + Pf1N1B4_1327 0.52 -2.0
1,522,810 + Pf1N1B4_1327 0.52 -0.5
1,522,811 - Pf1N1B4_1327 0.52 -1.3
1,522,811 - Pf1N1B4_1327 0.52 +0.6
1,522,811 - Pf1N1B4_1327 0.52 -0.8
1,522,811 - Pf1N1B4_1327 0.52 -0.1
1,522,811 - Pf1N1B4_1327 0.52 -0.4
1,522,811 - Pf1N1B4_1327 0.52 -0.9
1,522,811 - Pf1N1B4_1327 0.52 +1.8
1,522,811 - Pf1N1B4_1327 0.52 -0.5
1,522,811 - Pf1N1B4_1327 0.52 -0.8
1,522,811 - Pf1N1B4_1327 0.52 +0.1
1,522,811 - Pf1N1B4_1327 0.52 -0.8
1,522,811 - Pf1N1B4_1327 0.52 +1.3
1,522,811 - Pf1N1B4_1327 0.52 +0.9
1,522,819 + Pf1N1B4_1327 0.53 -0.5
1,522,820 - Pf1N1B4_1327 0.53 +0.1
1,522,903 + Pf1N1B4_1327 0.65 +0.3
1,522,904 - Pf1N1B4_1327 0.65 -0.3
1,522,904 - Pf1N1B4_1327 0.65 -0.8
1,522,904 - Pf1N1B4_1327 0.65 -0.7
1,522,904 - Pf1N1B4_1327 0.65 -1.1
1,522,928 - Pf1N1B4_1327 0.68 -0.2
1,523,054 - Pf1N1B4_1327 0.85 +0.0
1,523,054 - Pf1N1B4_1327 0.85 -0.8
1,523,054 - Pf1N1B4_1327 0.85 +0.5
1,523,054 - Pf1N1B4_1327 0.85 +0.3
1,523,361 + +0.5
1,523,361 + Pf1N1B4_1328 0.32 -1.3
1,523,361 + Pf1N1B4_1328 0.32 +1.5
1,523,361 + Pf1N1B4_1328 0.32 +0.9
1,523,362 - Pf1N1B4_1328 0.32 -1.3
1,523,362 - Pf1N1B4_1328 0.32 -0.8
1,523,362 - Pf1N1B4_1328 0.32 +0.2
1,523,362 - Pf1N1B4_1328 0.32 -1.1
1,523,751 + -0.1
1,523,751 + +1.5
1,523,751 + -0.7
1,523,751 + -0.5
1,523,752 - +0.5
1,523,752 - -0.5
1,523,775 + +0.5
1,523,775 + +0.3
1,523,775 + -0.8
1,523,775 + +0.5
1,523,776 - +0.3
1,523,776 - -1.2
1,523,776 - +0.0
1,523,776 - +1.5
1,523,776 - -1.0
1,523,776 - -0.7
1,523,776 - -0.6
1,523,834 + -2.0
1,523,834 + -1.5
1,523,834 + -0.1
1,523,835 - +0.2
1,523,835 - -1.5
1,523,835 - -0.8
1,523,835 - -1.5
1,523,835 - -1.7
1,523,837 + -0.3
1,523,837 + -1.2
1,523,838 - -2.5
1,523,838 - -2.1
1,523,838 - +1.5
1,523,838 - -0.8
1,523,838 - -2.2
1,523,890 + +1.7
1,523,891 - -0.5
1,524,283 - Pf1N1B4_1329 0.39 -1.7
1,524,295 + Pf1N1B4_1329 0.40 -1.1
1,524,295 + Pf1N1B4_1329 0.40 +1.8
1,524,295 + +1.5
1,524,295 + Pf1N1B4_1329 0.40 +0.0
1,524,295 + Pf1N1B4_1329 0.40 -0.3
1,524,295 + Pf1N1B4_1329 0.40 -1.0
1,524,295 + Pf1N1B4_1329 0.40 +0.5
1,524,296 - Pf1N1B4_1329 0.40 -0.6
1,524,296 - Pf1N1B4_1329 0.40 -0.1
1,524,296 - Pf1N1B4_1329 0.40 -0.8
1,524,296 - Pf1N1B4_1329 0.40 -1.5
1,524,296 - Pf1N1B4_1329 0.40 +0.2
1,524,296 - Pf1N1B4_1329 0.40 -2.5
1,524,296 - Pf1N1B4_1329 0.40 -0.8
1,524,296 - Pf1N1B4_1329 0.40 -2.0
1,524,296 - Pf1N1B4_1329 0.40 -1.1
1,524,296 - Pf1N1B4_1329 0.40 -0.3
1,524,296 - Pf1N1B4_1329 0.40 -1.5
1,524,296 - Pf1N1B4_1329 0.40 -0.8
1,524,296 - Pf1N1B4_1329 0.40 -0.5
1,524,301 + Pf1N1B4_1329 0.40 -0.5
1,524,302 - Pf1N1B4_1329 0.40 -2.4
1,524,302 - Pf1N1B4_1329 0.40 -0.6
1,524,302 - Pf1N1B4_1329 0.40 -2.3
1,524,302 - Pf1N1B4_1329 0.40 -2.1
1,524,302 - Pf1N1B4_1329 0.40 -2.4
1,524,758 + Pf1N1B4_1329 0.75 +0.3
1,524,758 + Pf1N1B4_1329 0.75 +0.5
1,524,759 - Pf1N1B4_1329 0.75 -0.1
1,524,759 - Pf1N1B4_1329 0.75 +1.5
1,524,759 - Pf1N1B4_1329 0.75 +2.1
1,524,759 - Pf1N1B4_1329 0.75 -0.3
1,525,065 + -0.3
1,525,066 - +0.5
1,525,145 - -0.9
1,525,201 - -0.1
1,525,201 - -0.1
1,525,208 - -0.2
1,525,236 - -0.3
1,525,377 - Pf1N1B4_1330 0.23 -0.5
1,525,377 - Pf1N1B4_1330 0.23 +0.6
1,525,377 - Pf1N1B4_1330 0.23 -1.1
1,525,383 + Pf1N1B4_1330 0.24 +0.7
1,525,384 - Pf1N1B4_1330 0.24 +0.5
1,525,448 + Pf1N1B4_1330 0.34 +0.5
1,525,464 + Pf1N1B4_1330 0.36 -0.8
1,525,729 - Pf1N1B4_1330 0.77 +1.1
1,525,731 - Pf1N1B4_1330 0.77 +0.0
1,525,731 - Pf1N1B4_1330 0.77 -1.3
1,525,759 + Pf1N1B4_1330 0.81 +2.2
1,525,825 - +0.5
1,525,825 - -0.2
1,525,825 - -0.3
1,525,882 - +0.7
1,525,975 + +0.3
1,526,016 + +1.1
1,526,016 + +0.9

Or see this region's nucleotide sequence