Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1313 and Pf1N1B4_1314 are separated by 38 nucleotides Pf1N1B4_1314 and Pf1N1B4_1315 are separated by 82 nucleotides Pf1N1B4_1315 and Pf1N1B4_1316 are separated by 151 nucleotides
Pf1N1B4_1313 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 1,496,027 to 1,497,037
_1313
Pf1N1B4_1314 - DNA-binding protein Fis, at 1,497,076 to 1,497,354
_1314
Pf1N1B4_1315 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 1,497,437 to 1,499,044
_1315
Pf1N1B4_1316 - Phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 1,499,196 to 1,500,491
_1316
Position (kb)
1497
1498
1499
1500 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.582 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.843 kb on + strand, within Pf1N1B4_1313 at 1496.843 kb on + strand, within Pf1N1B4_1313 at 1496.868 kb on + strand, within Pf1N1B4_1313 at 1496.868 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.996 kb on + strand at 1496.996 kb on + strand at 1496.996 kb on + strand at 1496.996 kb on + strand at 1497.056 kb on + strand at 1497.352 kb on + strand at 1497.615 kb on - strand, within Pf1N1B4_1315 at 1497.903 kb on - strand, within Pf1N1B4_1315 at 1497.944 kb on + strand, within Pf1N1B4_1315 at 1497.944 kb on + strand, within Pf1N1B4_1315 at 1497.944 kb on + strand, within Pf1N1B4_1315 at 1497.945 kb on - strand, within Pf1N1B4_1315 at 1497.945 kb on - strand, within Pf1N1B4_1315 at 1497.945 kb on - strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.361 kb on + strand, within Pf1N1B4_1315 at 1498.536 kb on + strand, within Pf1N1B4_1315 at 1498.536 kb on + strand, within Pf1N1B4_1315 at 1498.536 kb on + strand, within Pf1N1B4_1315 at 1498.589 kb on + strand, within Pf1N1B4_1315 at 1498.589 kb on + strand, within Pf1N1B4_1315 at 1498.589 kb on + strand, within Pf1N1B4_1315 at 1498.589 kb on + strand, within Pf1N1B4_1315 at 1498.590 kb on - strand, within Pf1N1B4_1315 at 1498.730 kb on + strand, within Pf1N1B4_1315 at 1498.730 kb on + strand, within Pf1N1B4_1315 at 1498.730 kb on + strand, within Pf1N1B4_1315 at 1498.730 kb on + strand, within Pf1N1B4_1315 at 1498.731 kb on - strand, within Pf1N1B4_1315 at 1498.731 kb on - strand, within Pf1N1B4_1315 at 1498.731 kb on - strand, within Pf1N1B4_1315 at 1498.735 kb on + strand, within Pf1N1B4_1315 at 1498.735 kb on + strand, within Pf1N1B4_1315 at 1498.735 kb on + strand, within Pf1N1B4_1315 at 1498.933 kb on - strand at 1499.173 kb on + strand at 1499.514 kb on - strand, within Pf1N1B4_1316 at 1499.592 kb on + strand, within Pf1N1B4_1316 at 1499.593 kb on - strand, within Pf1N1B4_1316 at 1499.616 kb on - strand, within Pf1N1B4_1316 at 1499.809 kb on + strand, within Pf1N1B4_1316
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 1,496,564 + Pf1N1B4_1313 0.53 -0.5 1,496,564 + Pf1N1B4_1313 0.53 -1.4 1,496,564 + Pf1N1B4_1313 0.53 -0.0 1,496,564 + Pf1N1B4_1313 0.53 -2.5 1,496,564 + Pf1N1B4_1313 0.53 +0.1 1,496,564 + Pf1N1B4_1313 0.53 +1.0 1,496,564 + Pf1N1B4_1313 0.53 -0.9 1,496,564 + Pf1N1B4_1313 0.53 +0.5 1,496,582 + Pf1N1B4_1313 0.55 -0.5 1,496,753 + Pf1N1B4_1313 0.72 +0.3 1,496,753 + Pf1N1B4_1313 0.72 +1.5 1,496,753 + Pf1N1B4_1313 0.72 -0.1 1,496,843 + Pf1N1B4_1313 0.81 -0.1 1,496,843 + Pf1N1B4_1313 0.81 +0.6 1,496,868 + Pf1N1B4_1313 0.83 +0.8 1,496,868 + Pf1N1B4_1313 0.83 -1.7 1,496,891 + Pf1N1B4_1313 0.85 +1.5 1,496,891 + Pf1N1B4_1313 0.85 -0.4 1,496,891 + Pf1N1B4_1313 0.85 +1.5 1,496,891 + Pf1N1B4_1313 0.85 -1.3 1,496,969 + +0.8 1,496,969 + -0.3 1,496,969 + -0.8 1,496,969 + +0.1 1,496,969 + -1.6 1,496,996 + -0.1 1,496,996 + +1.7 1,496,996 + +1.2 1,496,996 + +0.7 1,497,056 + -0.9 1,497,352 + +0.5 1,497,615 - Pf1N1B4_1315 0.11 -1.5 1,497,903 - Pf1N1B4_1315 0.29 -0.5 1,497,944 + Pf1N1B4_1315 0.32 -3.6 1,497,944 + Pf1N1B4_1315 0.32 -2.0 1,497,944 + Pf1N1B4_1315 0.32 -3.1 1,497,945 - Pf1N1B4_1315 0.32 -1.2 1,497,945 - Pf1N1B4_1315 0.32 -3.0 1,497,945 - Pf1N1B4_1315 0.32 -0.3 1,498,016 + Pf1N1B4_1315 0.36 -2.2 1,498,016 + Pf1N1B4_1315 0.36 -2.4 1,498,016 + Pf1N1B4_1315 0.36 -1.7 1,498,016 + Pf1N1B4_1315 0.36 -0.9 1,498,016 + Pf1N1B4_1315 0.36 -1.3 1,498,016 + Pf1N1B4_1315 0.36 -1.7 1,498,016 + Pf1N1B4_1315 0.36 -2.8 1,498,016 + Pf1N1B4_1315 0.36 -2.1 1,498,017 - Pf1N1B4_1315 0.36 -0.7 1,498,017 - Pf1N1B4_1315 0.36 -1.8 1,498,017 - Pf1N1B4_1315 0.36 -0.1 1,498,017 - Pf1N1B4_1315 0.36 -0.9 1,498,154 + Pf1N1B4_1315 0.45 -2.2 1,498,154 + Pf1N1B4_1315 0.45 -2.2 1,498,154 + Pf1N1B4_1315 0.45 -3.9 1,498,154 + Pf1N1B4_1315 0.45 -1.1 1,498,154 + Pf1N1B4_1315 0.45 -1.9 1,498,361 + Pf1N1B4_1315 0.57 -2.2 1,498,536 + Pf1N1B4_1315 0.68 +0.1 1,498,536 + Pf1N1B4_1315 0.68 -2.1 1,498,536 + Pf1N1B4_1315 0.68 -0.5 1,498,589 + Pf1N1B4_1315 0.72 +0.5 1,498,589 + Pf1N1B4_1315 0.72 -0.9 1,498,589 + Pf1N1B4_1315 0.72 -3.7 1,498,589 + Pf1N1B4_1315 0.72 -0.1 1,498,590 - Pf1N1B4_1315 0.72 +0.0 1,498,730 + Pf1N1B4_1315 0.80 -1.3 1,498,730 + Pf1N1B4_1315 0.80 -0.5 1,498,730 + Pf1N1B4_1315 0.80 -1.5 1,498,730 + Pf1N1B4_1315 0.80 -0.5 1,498,731 - Pf1N1B4_1315 0.80 -0.7 1,498,731 - Pf1N1B4_1315 0.80 -0.1 1,498,731 - Pf1N1B4_1315 0.80 -2.4 1,498,735 + Pf1N1B4_1315 0.81 -1.1 1,498,735 + Pf1N1B4_1315 0.81 -2.3 1,498,735 + Pf1N1B4_1315 0.81 -1.1 1,498,933 - +0.9 1,499,173 + +0.2 1,499,514 - Pf1N1B4_1316 0.25 -1.7 1,499,592 + Pf1N1B4_1316 0.31 -1.3 1,499,593 - Pf1N1B4_1316 0.31 -0.9 1,499,616 - Pf1N1B4_1316 0.32 -1.1 1,499,809 + Pf1N1B4_1316 0.47 -1.9
Or see this region's nucleotide sequence