Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_12 and Pf1N1B4_13 are separated by 93 nucleotides Pf1N1B4_13 and Pf1N1B4_14 are separated by 152 nucleotides Pf1N1B4_14 and Pf1N1B4_15 are separated by 11 nucleotides
Pf1N1B4_12 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 13,273 to 14,355
_12
Pf1N1B4_13 - SAM-dependent methyltransferase YafE (UbiE paralog), at 14,449 to 15,216
_13
Pf1N1B4_14 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 15,369 to 16,013
_14
Pf1N1B4_15 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 16,025 to 17,443
_15
Position (kb) 14
15
16 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand at 13.527 kb on - strand, within Pf1N1B4_12 at 13.527 kb on - strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.935 kb on - strand, within Pf1N1B4_12 at 14.150 kb on - strand, within Pf1N1B4_12 at 14.261 kb on + strand at 14.261 kb on + strand at 14.262 kb on - strand at 14.422 kb on + strand at 14.481 kb on + strand at 14.513 kb on + strand at 14.513 kb on + strand at 14.606 kb on + strand, within Pf1N1B4_13 at 14.606 kb on + strand, within Pf1N1B4_13 at 14.691 kb on - strand, within Pf1N1B4_13 at 14.691 kb on - strand, within Pf1N1B4_13 at 14.738 kb on + strand, within Pf1N1B4_13 at 14.739 kb on - strand, within Pf1N1B4_13 at 14.972 kb on - strand, within Pf1N1B4_13 at 14.972 kb on - strand, within Pf1N1B4_13 at 15.152 kb on + strand at 15.152 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.185 kb on - strand at 15.187 kb on + strand at 15.679 kb on + strand, within Pf1N1B4_14 at 15.680 kb on - strand, within Pf1N1B4_14 at 15.680 kb on - strand, within Pf1N1B4_14 at 16.026 kb on + strand at 16.027 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 13,526 + Pf1N1B4_12 0.23 -2.4 13,526 + +0.0 13,526 + Pf1N1B4_12 0.23 -2.3 13,526 + Pf1N1B4_12 0.23 -2.6 13,526 + Pf1N1B4_12 0.23 -1.1 13,526 + Pf1N1B4_12 0.23 -3.5 13,526 + Pf1N1B4_12 0.23 -1.3 13,526 + -1.8 13,527 - Pf1N1B4_12 0.23 -1.3 13,527 - Pf1N1B4_12 0.23 -2.2 13,691 + Pf1N1B4_12 0.39 -2.4 13,691 + Pf1N1B4_12 0.39 -1.0 13,691 + Pf1N1B4_12 0.39 -2.7 13,691 + Pf1N1B4_12 0.39 -4.4 13,691 + +1.0 13,691 + Pf1N1B4_12 0.39 -2.3 13,691 + Pf1N1B4_12 0.39 -2.0 13,691 + Pf1N1B4_12 0.39 +0.4 13,691 + Pf1N1B4_12 0.39 -3.2 13,691 + Pf1N1B4_12 0.39 -1.4 13,692 - Pf1N1B4_12 0.39 -0.4 13,692 - Pf1N1B4_12 0.39 -1.6 13,692 - Pf1N1B4_12 0.39 -1.8 13,692 - Pf1N1B4_12 0.39 -1.0 13,692 - Pf1N1B4_12 0.39 -1.8 13,881 - Pf1N1B4_12 0.56 -0.6 13,881 - Pf1N1B4_12 0.56 +0.0 13,881 - Pf1N1B4_12 0.56 -0.6 13,934 + Pf1N1B4_12 0.61 -1.0 13,934 + Pf1N1B4_12 0.61 -1.2 13,934 + Pf1N1B4_12 0.61 -1.3 13,935 - Pf1N1B4_12 0.61 -1.0 14,150 - Pf1N1B4_12 0.81 -2.0 14,261 + -0.6 14,261 + -1.6 14,262 - -0.3 14,422 + -1.8 14,481 + -2.0 14,513 + -1.4 14,513 + -1.7 14,606 + Pf1N1B4_13 0.20 -0.6 14,606 + Pf1N1B4_13 0.20 -2.9 14,691 - Pf1N1B4_13 0.32 -0.3 14,691 - Pf1N1B4_13 0.32 -0.6 14,738 + Pf1N1B4_13 0.38 -1.3 14,739 - Pf1N1B4_13 0.38 -0.6 14,972 - Pf1N1B4_13 0.68 -0.1 14,972 - Pf1N1B4_13 0.68 +0.0 15,152 + -2.3 15,152 + -1.3 15,184 + +0.1 15,184 + -0.3 15,184 + +1.6 15,185 - -0.4 15,187 + -1.6 15,679 + Pf1N1B4_14 0.48 -1.3 15,680 - Pf1N1B4_14 0.48 -1.6 15,680 - Pf1N1B4_14 0.48 -2.3 16,026 + -5.0 16,027 - +1.8
Or see this region's nucleotide sequence