Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1066 and Pf1N1B4_1067 are separated by 126 nucleotides Pf1N1B4_1067 and Pf1N1B4_1068 are separated by 128 nucleotides
Pf1N1B4_1066 - Histidinol dehydrogenase (EC 1.1.1.23), at 1,201,450 to 1,202,775
_1066
Pf1N1B4_1067 - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 1,202,902 to 1,203,537
_1067
Pf1N1B4_1068 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 1,203,666 to 1,204,931
_1068
Position (kb)
1202
1203
1204 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1202.790 kb on - strand at 1202.790 kb on - strand at 1202.790 kb on - strand at 1202.790 kb on - strand at 1202.821 kb on - strand at 1202.821 kb on - strand at 1202.821 kb on - strand at 1203.128 kb on + strand, within Pf1N1B4_1067 at 1203.129 kb on - strand, within Pf1N1B4_1067 at 1203.167 kb on + strand at 1203.171 kb on - strand, within Pf1N1B4_1067 at 1203.290 kb on + strand, within Pf1N1B4_1067 at 1203.290 kb on + strand, within Pf1N1B4_1067 at 1203.290 kb on + strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.309 kb on - strand, within Pf1N1B4_1067 at 1203.365 kb on + strand, within Pf1N1B4_1067 at 1203.365 kb on + strand at 1203.365 kb on + strand, within Pf1N1B4_1067 at 1203.366 kb on - strand, within Pf1N1B4_1067 at 1203.366 kb on - strand, within Pf1N1B4_1067 at 1203.562 kb on + strand at 1203.594 kb on + strand at 1203.594 kb on + strand at 1203.594 kb on + strand at 1203.594 kb on + strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.667 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 1,202,790 - -0.6 1,202,790 - +0.4 1,202,790 - -1.4 1,202,790 - -0.1 1,202,821 - -1.6 1,202,821 - -0.2 1,202,821 - -1.2 1,203,128 + Pf1N1B4_1067 0.36 -0.2 1,203,129 - Pf1N1B4_1067 0.36 -1.9 1,203,167 + -0.6 1,203,171 - Pf1N1B4_1067 0.42 -1.9 1,203,290 + Pf1N1B4_1067 0.61 -0.9 1,203,290 + Pf1N1B4_1067 0.61 -3.1 1,203,290 + Pf1N1B4_1067 0.61 -3.6 1,203,291 - Pf1N1B4_1067 0.61 -0.2 1,203,291 - Pf1N1B4_1067 0.61 -4.7 1,203,291 - Pf1N1B4_1067 0.61 -0.2 1,203,291 - Pf1N1B4_1067 0.61 -2.6 1,203,291 - Pf1N1B4_1067 0.61 -2.2 1,203,291 - Pf1N1B4_1067 0.61 -2.7 1,203,309 - Pf1N1B4_1067 0.64 -2.2 1,203,365 + Pf1N1B4_1067 0.73 -2.9 1,203,365 + -1.8 1,203,365 + Pf1N1B4_1067 0.73 -2.9 1,203,366 - Pf1N1B4_1067 0.73 +1.5 1,203,366 - Pf1N1B4_1067 0.73 -2.8 1,203,562 + -1.8 1,203,594 + -1.4 1,203,594 + -0.9 1,203,594 + +0.1 1,203,594 + -0.3 1,203,595 - -0.9 1,203,595 - +1.4 1,203,595 - +0.2 1,203,595 - +0.4 1,203,595 - +0.4 1,203,595 - +1.4 1,203,667 + -3.4
Or see this region's nucleotide sequence