Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1002 and Pf1N1B4_1003 are separated by 373 nucleotides Pf1N1B4_1003 and Pf1N1B4_1004 are separated by 239 nucleotides
Pf1N1B4_1002 - LysR family transcriptional regulator QseA, at 1,137,186 to 1,138,154
_1002
Pf1N1B4_1003 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 1,138,528 to 1,138,734
_1003
Pf1N1B4_1004 - Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 1,138,974 to 1,139,948
_1004
Position (kb)
1138
1139 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1137.601 kb on - strand, within Pf1N1B4_1002 at 1137.672 kb on + strand, within Pf1N1B4_1002 at 1137.672 kb on + strand, within Pf1N1B4_1002 at 1137.672 kb on + strand, within Pf1N1B4_1002 at 1137.672 kb on + strand at 1137.673 kb on - strand, within Pf1N1B4_1002 at 1137.673 kb on - strand, within Pf1N1B4_1002 at 1137.673 kb on - strand, within Pf1N1B4_1002 at 1137.699 kb on + strand, within Pf1N1B4_1002 at 1137.699 kb on + strand, within Pf1N1B4_1002 at 1137.699 kb on + strand, within Pf1N1B4_1002 at 1137.700 kb on - strand, within Pf1N1B4_1002 at 1137.703 kb on + strand, within Pf1N1B4_1002 at 1137.703 kb on + strand, within Pf1N1B4_1002 at 1137.703 kb on + strand, within Pf1N1B4_1002 at 1137.703 kb on + strand, within Pf1N1B4_1002 at 1137.703 kb on + strand, within Pf1N1B4_1002 at 1137.704 kb on - strand, within Pf1N1B4_1002 at 1137.919 kb on - strand, within Pf1N1B4_1002 at 1137.973 kb on - strand, within Pf1N1B4_1002 at 1137.973 kb on - strand, within Pf1N1B4_1002 at 1137.973 kb on - strand, within Pf1N1B4_1002 at 1137.973 kb on - strand, within Pf1N1B4_1002 at 1137.997 kb on - strand, within Pf1N1B4_1002 at 1138.005 kb on + strand, within Pf1N1B4_1002 at 1138.005 kb on + strand, within Pf1N1B4_1002 at 1138.006 kb on - strand, within Pf1N1B4_1002 at 1138.021 kb on - strand, within Pf1N1B4_1002 at 1138.053 kb on + strand, within Pf1N1B4_1002 at 1138.053 kb on + strand, within Pf1N1B4_1002 at 1138.053 kb on + strand, within Pf1N1B4_1002 at 1138.054 kb on - strand, within Pf1N1B4_1002 at 1138.078 kb on + strand at 1138.078 kb on + strand at 1138.110 kb on + strand at 1138.157 kb on + strand at 1138.157 kb on + strand at 1138.170 kb on - strand at 1138.314 kb on + strand at 1138.314 kb on + strand at 1138.343 kb on + strand at 1138.486 kb on + strand at 1138.486 kb on + strand at 1138.486 kb on + strand at 1138.486 kb on + strand at 1138.486 kb on + strand at 1138.487 kb on - strand at 1138.487 kb on - strand at 1138.487 kb on - strand at 1138.487 kb on - strand at 1138.565 kb on + strand at 1138.565 kb on + strand, within Pf1N1B4_1003 at 1138.566 kb on - strand, within Pf1N1B4_1003 at 1138.573 kb on + strand, within Pf1N1B4_1003 at 1138.650 kb on + strand, within Pf1N1B4_1003 at 1138.650 kb on + strand, within Pf1N1B4_1003 at 1138.651 kb on - strand, within Pf1N1B4_1003 at 1138.651 kb on - strand at 1138.651 kb on - strand, within Pf1N1B4_1003 at 1138.651 kb on - strand, within Pf1N1B4_1003 at 1138.834 kb on + strand at 1138.834 kb on + strand at 1138.834 kb on + strand at 1138.834 kb on + strand at 1138.835 kb on - strand at 1138.835 kb on - strand at 1138.835 kb on - strand at 1138.835 kb on - strand at 1138.909 kb on - strand at 1139.211 kb on + strand, within Pf1N1B4_1004 at 1139.212 kb on - strand, within Pf1N1B4_1004 at 1139.212 kb on - strand, within Pf1N1B4_1004 at 1139.212 kb on - strand, within Pf1N1B4_1004 at 1139.224 kb on - strand, within Pf1N1B4_1004 at 1139.306 kb on + strand, within Pf1N1B4_1004 at 1139.306 kb on + strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.489 kb on + strand, within Pf1N1B4_1004 at 1139.490 kb on - strand, within Pf1N1B4_1004 at 1139.490 kb on - strand, within Pf1N1B4_1004 at 1139.493 kb on + strand, within Pf1N1B4_1004
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 1,137,601 - Pf1N1B4_1002 0.43 +1.1 1,137,672 + Pf1N1B4_1002 0.50 +2.4 1,137,672 + Pf1N1B4_1002 0.50 -1.1 1,137,672 + Pf1N1B4_1002 0.50 +0.5 1,137,672 + +1.4 1,137,673 - Pf1N1B4_1002 0.50 +0.1 1,137,673 - Pf1N1B4_1002 0.50 -0.2 1,137,673 - Pf1N1B4_1002 0.50 -2.2 1,137,699 + Pf1N1B4_1002 0.53 +1.2 1,137,699 + Pf1N1B4_1002 0.53 +1.7 1,137,699 + Pf1N1B4_1002 0.53 -0.4 1,137,700 - Pf1N1B4_1002 0.53 -0.0 1,137,703 + Pf1N1B4_1002 0.53 -1.2 1,137,703 + Pf1N1B4_1002 0.53 -0.6 1,137,703 + Pf1N1B4_1002 0.53 +0.5 1,137,703 + Pf1N1B4_1002 0.53 -0.5 1,137,703 + Pf1N1B4_1002 0.53 -0.6 1,137,704 - Pf1N1B4_1002 0.53 -0.6 1,137,919 - Pf1N1B4_1002 0.76 +0.4 1,137,973 - Pf1N1B4_1002 0.81 +0.1 1,137,973 - Pf1N1B4_1002 0.81 +0.8 1,137,973 - Pf1N1B4_1002 0.81 +0.1 1,137,973 - Pf1N1B4_1002 0.81 -1.0 1,137,997 - Pf1N1B4_1002 0.84 -0.8 1,138,005 + Pf1N1B4_1002 0.85 -0.0 1,138,005 + Pf1N1B4_1002 0.85 +0.8 1,138,006 - Pf1N1B4_1002 0.85 +0.9 1,138,021 - Pf1N1B4_1002 0.86 +0.3 1,138,053 + Pf1N1B4_1002 0.89 -0.1 1,138,053 + Pf1N1B4_1002 0.89 -0.6 1,138,053 + Pf1N1B4_1002 0.89 -0.2 1,138,054 - Pf1N1B4_1002 0.90 +0.4 1,138,078 + -0.3 1,138,078 + +0.2 1,138,110 + +1.2 1,138,157 + -0.9 1,138,157 + +0.4 1,138,170 - -1.3 1,138,314 + +1.4 1,138,314 + +0.3 1,138,343 + -1.4 1,138,486 + +0.1 1,138,486 + -0.2 1,138,486 + +0.4 1,138,486 + -0.2 1,138,486 + +1.4 1,138,487 - -0.3 1,138,487 - +0.3 1,138,487 - +0.2 1,138,487 - -1.2 1,138,565 + +2.4 1,138,565 + Pf1N1B4_1003 0.18 +1.7 1,138,566 - Pf1N1B4_1003 0.18 -1.6 1,138,573 + Pf1N1B4_1003 0.22 -1.4 1,138,650 + Pf1N1B4_1003 0.59 -0.4 1,138,650 + Pf1N1B4_1003 0.59 -1.8 1,138,651 - Pf1N1B4_1003 0.59 -0.7 1,138,651 - -2.1 1,138,651 - Pf1N1B4_1003 0.59 -1.8 1,138,651 - Pf1N1B4_1003 0.59 +1.8 1,138,834 + +0.4 1,138,834 + -1.0 1,138,834 + +0.7 1,138,834 + -2.9 1,138,835 - -0.8 1,138,835 - +0.1 1,138,835 - +1.4 1,138,835 - -0.8 1,138,909 - +2.4 1,139,211 + Pf1N1B4_1004 0.24 +0.5 1,139,212 - Pf1N1B4_1004 0.24 -0.6 1,139,212 - Pf1N1B4_1004 0.24 -0.6 1,139,212 - Pf1N1B4_1004 0.24 -0.4 1,139,224 - Pf1N1B4_1004 0.26 +0.2 1,139,306 + Pf1N1B4_1004 0.34 -0.7 1,139,306 + Pf1N1B4_1004 0.34 +0.1 1,139,307 - Pf1N1B4_1004 0.34 -0.6 1,139,307 - Pf1N1B4_1004 0.34 -0.6 1,139,307 - Pf1N1B4_1004 0.34 -0.8 1,139,489 + Pf1N1B4_1004 0.53 -0.9 1,139,490 - Pf1N1B4_1004 0.53 -0.6 1,139,490 - Pf1N1B4_1004 0.53 +0.1 1,139,493 + Pf1N1B4_1004 0.53 -0.4
Or see this region's nucleotide sequence