Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37470 and RR42_RS37475 are separated by 54 nucleotides RR42_RS37475 and RR42_RS37480 are separated by 135 nucleotides
RR42_RS37470: RR42_RS37470 - permease, at 3,885,469 to 3,886,239
_RS37470
RR42_RS37475: RR42_RS37475 - peptide chain release factor 3, at 3,886,294 to 3,887,868
_RS37475
RR42_RS37480: RR42_RS37480 - membrane protein, at 3,888,004 to 3,888,969
_RS37480
Position (kb)
3886
3887
3888 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3885.413 kb on + strand at 3885.414 kb on - strand at 3885.414 kb on - strand at 3885.439 kb on + strand at 3885.439 kb on + strand at 3885.439 kb on + strand at 3885.439 kb on + strand at 3885.517 kb on + strand at 3885.550 kb on + strand, within RR42_RS37470 at 3885.551 kb on - strand, within RR42_RS37470 at 3885.551 kb on - strand, within RR42_RS37470 at 3885.611 kb on - strand, within RR42_RS37470 at 3885.641 kb on + strand, within RR42_RS37470 at 3885.813 kb on - strand, within RR42_RS37470 at 3885.813 kb on - strand, within RR42_RS37470 at 3885.944 kb on - strand, within RR42_RS37470 at 3885.944 kb on - strand, within RR42_RS37470 at 3885.944 kb on - strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.952 kb on + strand, within RR42_RS37470 at 3885.953 kb on - strand, within RR42_RS37470 at 3885.953 kb on - strand, within RR42_RS37470 at 3886.017 kb on + strand, within RR42_RS37470 at 3886.037 kb on + strand, within RR42_RS37470 at 3886.037 kb on + strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.038 kb on - strand, within RR42_RS37470 at 3886.057 kb on + strand, within RR42_RS37470 at 3886.058 kb on - strand, within RR42_RS37470 at 3886.219 kb on + strand at 3886.219 kb on + strand at 3886.219 kb on + strand at 3886.220 kb on - strand at 3886.238 kb on - strand at 3886.238 kb on - strand at 3886.379 kb on + strand at 3886.379 kb on + strand at 3886.379 kb on + strand at 3886.408 kb on + strand at 3886.409 kb on - strand at 3886.409 kb on - strand at 3886.514 kb on + strand, within RR42_RS37475 at 3886.514 kb on + strand, within RR42_RS37475 at 3886.514 kb on + strand, within RR42_RS37475 at 3886.515 kb on - strand, within RR42_RS37475 at 3886.573 kb on + strand, within RR42_RS37475 at 3886.806 kb on - strand, within RR42_RS37475 at 3886.822 kb on - strand, within RR42_RS37475 at 3886.855 kb on - strand, within RR42_RS37475 at 3887.120 kb on + strand, within RR42_RS37475 at 3887.121 kb on - strand, within RR42_RS37475 at 3887.327 kb on + strand, within RR42_RS37475 at 3887.327 kb on + strand, within RR42_RS37475 at 3887.327 kb on + strand, within RR42_RS37475 at 3887.327 kb on + strand, within RR42_RS37475 at 3887.328 kb on - strand, within RR42_RS37475 at 3887.454 kb on + strand, within RR42_RS37475 at 3887.454 kb on + strand, within RR42_RS37475 at 3887.455 kb on - strand, within RR42_RS37475 at 3887.576 kb on + strand, within RR42_RS37475 at 3887.576 kb on + strand, within RR42_RS37475 at 3887.576 kb on + strand, within RR42_RS37475 at 3887.576 kb on + strand, within RR42_RS37475 at 3887.577 kb on - strand, within RR42_RS37475 at 3887.577 kb on - strand, within RR42_RS37475 at 3887.577 kb on - strand, within RR42_RS37475 at 3887.800 kb on - strand at 3887.855 kb on - strand at 3888.086 kb on + strand at 3888.195 kb on + strand, within RR42_RS37480 at 3888.195 kb on + strand, within RR42_RS37480 at 3888.196 kb on - strand, within RR42_RS37480 at 3888.206 kb on + strand, within RR42_RS37480 at 3888.813 kb on + strand, within RR42_RS37480 at 3888.813 kb on + strand, within RR42_RS37480 at 3888.814 kb on - strand, within RR42_RS37480 at 3888.814 kb on - strand, within RR42_RS37480 at 3888.814 kb on - strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,885,413 + -1.0 3,885,414 - +1.1 3,885,414 - +0.4 3,885,439 + -2.1 3,885,439 + -1.6 3,885,439 + +0.4 3,885,439 + +0.4 3,885,517 + -0.4 3,885,550 + RR42_RS37470 0.11 -0.2 3,885,551 - RR42_RS37470 0.11 -0.7 3,885,551 - RR42_RS37470 0.11 +0.2 3,885,611 - RR42_RS37470 0.18 -0.0 3,885,641 + RR42_RS37470 0.22 -0.2 3,885,813 - RR42_RS37470 0.45 +0.4 3,885,813 - RR42_RS37470 0.45 -0.2 3,885,944 - RR42_RS37470 0.62 +0.1 3,885,944 - RR42_RS37470 0.62 -0.6 3,885,944 - RR42_RS37470 0.62 +0.3 3,885,952 + RR42_RS37470 0.63 +0.8 3,885,952 + RR42_RS37470 0.63 -0.3 3,885,952 + RR42_RS37470 0.63 -0.1 3,885,952 + RR42_RS37470 0.63 +0.4 3,885,952 + RR42_RS37470 0.63 -0.4 3,885,953 - RR42_RS37470 0.63 +0.7 3,885,953 - RR42_RS37470 0.63 +0.3 3,886,017 + RR42_RS37470 0.71 -0.5 3,886,037 + RR42_RS37470 0.74 +0.3 3,886,037 + RR42_RS37470 0.74 -0.4 3,886,038 - RR42_RS37470 0.74 +1.5 3,886,038 - RR42_RS37470 0.74 -0.9 3,886,038 - RR42_RS37470 0.74 -0.3 3,886,038 - RR42_RS37470 0.74 +0.6 3,886,038 - RR42_RS37470 0.74 -1.2 3,886,057 + RR42_RS37470 0.76 +0.4 3,886,058 - RR42_RS37470 0.76 -0.6 3,886,219 + -0.3 3,886,219 + -1.3 3,886,219 + +0.4 3,886,220 - -0.4 3,886,238 - +0.9 3,886,238 - +1.2 3,886,379 + -0.8 3,886,379 + +1.9 3,886,379 + +0.7 3,886,408 + +1.7 3,886,409 - +0.2 3,886,409 - -1.1 3,886,514 + RR42_RS37475 0.14 +0.9 3,886,514 + RR42_RS37475 0.14 +0.9 3,886,514 + RR42_RS37475 0.14 +0.5 3,886,515 - RR42_RS37475 0.14 -1.7 3,886,573 + RR42_RS37475 0.18 -0.8 3,886,806 - RR42_RS37475 0.33 +1.7 3,886,822 - RR42_RS37475 0.34 +1.4 3,886,855 - RR42_RS37475 0.36 +2.2 3,887,120 + RR42_RS37475 0.52 +0.2 3,887,121 - RR42_RS37475 0.53 +0.9 3,887,327 + RR42_RS37475 0.66 -1.9 3,887,327 + RR42_RS37475 0.66 +0.6 3,887,327 + RR42_RS37475 0.66 +0.5 3,887,327 + RR42_RS37475 0.66 +0.4 3,887,328 - RR42_RS37475 0.66 +0.9 3,887,454 + RR42_RS37475 0.74 -0.6 3,887,454 + RR42_RS37475 0.74 +0.3 3,887,455 - RR42_RS37475 0.74 +1.1 3,887,576 + RR42_RS37475 0.81 +0.7 3,887,576 + RR42_RS37475 0.81 +0.6 3,887,576 + RR42_RS37475 0.81 -0.5 3,887,576 + RR42_RS37475 0.81 +0.1 3,887,577 - RR42_RS37475 0.81 +0.4 3,887,577 - RR42_RS37475 0.81 +1.4 3,887,577 - RR42_RS37475 0.81 +0.7 3,887,800 - +1.1 3,887,855 - +0.6 3,888,086 + -0.6 3,888,195 + RR42_RS37480 0.20 -2.0 3,888,195 + RR42_RS37480 0.20 -0.6 3,888,196 - RR42_RS37480 0.20 -0.7 3,888,206 + RR42_RS37480 0.21 -0.4 3,888,813 + RR42_RS37480 0.84 -0.8 3,888,813 + RR42_RS37480 0.84 -0.4 3,888,814 - RR42_RS37480 0.84 -0.0 3,888,814 - RR42_RS37480 0.84 +0.5 3,888,814 - RR42_RS37480 0.84 -1.1 3,888,815 + RR42_RS37480 0.84 -0.6 3,888,815 + RR42_RS37480 0.84 -1.1 3,888,815 + RR42_RS37480 0.84 -0.2 3,888,815 + RR42_RS37480 0.84 -0.6 3,888,815 + RR42_RS37480 0.84 -2.8 3,888,815 + RR42_RS37480 0.84 -0.1 3,888,815 + RR42_RS37480 0.84 -0.1 3,888,815 + RR42_RS37480 0.84 +0.9 3,888,816 - RR42_RS37480 0.84 +0.0 3,888,816 - RR42_RS37480 0.84 -1.3 3,888,816 - RR42_RS37480 0.84 +0.6 3,888,816 - RR42_RS37480 0.84 +2.2 3,888,816 - RR42_RS37480 0.84 +0.6 3,888,816 - RR42_RS37480 0.84 -0.3 3,888,816 - RR42_RS37480 0.84 -0.9 3,888,816 - RR42_RS37480 0.84 +0.4 3,888,816 - RR42_RS37480 0.84 +1.6
Or see this region's nucleotide sequence