Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36010 and RR42_RS36015 are separated by 9 nucleotides RR42_RS36015 and RR42_RS36020 are separated by 366 nucleotides RR42_RS36020 and RR42_RS36025 are separated by 694 nucleotides
RR42_RS36010: RR42_RS36010 - peptidase, at 3,526,711 to 3,527,214
_RS36010
RR42_RS36015: RR42_RS36015 - pilus assembly protein, at 3,527,224 to 3,527,412
_RS36015
RR42_RS36020: RR42_RS36020 - hemagglutinin, at 3,527,779 to 3,528,954
_RS36020
RR42_RS36025: RR42_RS36025 - hemagglutinin, at 3,529,649 to 3,531,103
_RS36025
Position (kb)
3527
3528
3529 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3527.019 kb on - strand, within RR42_RS36010 at 3527.226 kb on - strand at 3527.299 kb on + strand, within RR42_RS36015 at 3527.300 kb on - strand, within RR42_RS36015 at 3527.300 kb on - strand, within RR42_RS36015 at 3527.300 kb on - strand, within RR42_RS36015 at 3527.319 kb on + strand, within RR42_RS36015 at 3527.319 kb on + strand, within RR42_RS36015 at 3527.354 kb on + strand, within RR42_RS36015 at 3527.396 kb on - strand at 3527.435 kb on - strand at 3527.493 kb on + strand at 3527.497 kb on - strand at 3527.524 kb on + strand at 3527.527 kb on - strand at 3527.541 kb on + strand at 3527.541 kb on + strand at 3527.541 kb on + strand at 3527.542 kb on - strand at 3527.542 kb on - strand at 3527.542 kb on - strand at 3527.542 kb on - strand at 3527.551 kb on - strand at 3527.566 kb on + strand at 3527.566 kb on + strand at 3527.567 kb on - strand at 3527.569 kb on - strand at 3527.569 kb on - strand at 3527.583 kb on - strand at 3527.646 kb on + strand at 3527.688 kb on - strand at 3527.729 kb on + strand at 3527.730 kb on - strand at 3527.736 kb on + strand at 3527.736 kb on + strand at 3527.737 kb on - strand at 3527.755 kb on + strand at 3527.765 kb on - strand at 3527.765 kb on - strand at 3527.765 kb on - strand at 3528.288 kb on + strand, within RR42_RS36020 at 3528.455 kb on - strand, within RR42_RS36020 at 3528.555 kb on + strand, within RR42_RS36020 at 3528.556 kb on - strand, within RR42_RS36020 at 3528.556 kb on - strand, within RR42_RS36020 at 3528.625 kb on + strand, within RR42_RS36020 at 3528.625 kb on + strand, within RR42_RS36020 at 3528.626 kb on - strand, within RR42_RS36020 at 3528.626 kb on - strand, within RR42_RS36020 at 3528.753 kb on + strand, within RR42_RS36020 at 3528.754 kb on - strand, within RR42_RS36020 at 3528.754 kb on - strand, within RR42_RS36020 at 3529.052 kb on + strand at 3529.053 kb on - strand at 3529.059 kb on - strand at 3529.210 kb on + strand at 3529.210 kb on + strand at 3529.211 kb on - strand at 3529.269 kb on - strand at 3529.273 kb on + strand at 3529.274 kb on - strand at 3529.274 kb on - strand at 3529.274 kb on - strand at 3529.277 kb on + strand at 3529.410 kb on + strand at 3529.411 kb on - strand at 3529.414 kb on + strand at 3529.422 kb on - strand at 3529.422 kb on - strand at 3529.469 kb on + strand at 3529.516 kb on + strand at 3529.516 kb on + strand at 3529.517 kb on - strand at 3529.517 kb on - strand at 3529.655 kb on - strand at 3529.655 kb on - strand at 3529.742 kb on - strand at 3529.909 kb on + strand, within RR42_RS36025 at 3529.909 kb on + strand, within RR42_RS36025 at 3529.910 kb on - strand, within RR42_RS36025 at 3529.910 kb on - strand, within RR42_RS36025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,527,019 - RR42_RS36010 0.61 -0.0 3,527,226 - +0.7 3,527,299 + RR42_RS36015 0.40 +0.8 3,527,300 - RR42_RS36015 0.40 -0.5 3,527,300 - RR42_RS36015 0.40 +0.7 3,527,300 - RR42_RS36015 0.40 +1.3 3,527,319 + RR42_RS36015 0.50 +1.0 3,527,319 + RR42_RS36015 0.50 +0.4 3,527,354 + RR42_RS36015 0.69 +0.3 3,527,396 - +0.2 3,527,435 - -0.6 3,527,493 + +0.4 3,527,497 - +0.0 3,527,524 + -0.0 3,527,527 - +0.1 3,527,541 + +0.4 3,527,541 + -2.5 3,527,541 + -1.5 3,527,542 - -0.8 3,527,542 - +0.8 3,527,542 - -1.7 3,527,542 - -0.2 3,527,551 - -0.1 3,527,566 + -0.7 3,527,566 + +1.7 3,527,567 - +1.0 3,527,569 - -0.3 3,527,569 - +0.0 3,527,583 - -0.6 3,527,646 + +0.3 3,527,688 - -0.3 3,527,729 + -1.6 3,527,730 - -0.4 3,527,736 + +0.8 3,527,736 + +0.1 3,527,737 - +2.1 3,527,755 + -0.2 3,527,765 - +1.9 3,527,765 - -1.5 3,527,765 - +0.6 3,528,288 + RR42_RS36020 0.43 -0.1 3,528,455 - RR42_RS36020 0.57 -0.3 3,528,555 + RR42_RS36020 0.66 +0.4 3,528,556 - RR42_RS36020 0.66 +0.6 3,528,556 - RR42_RS36020 0.66 -1.4 3,528,625 + RR42_RS36020 0.72 +0.7 3,528,625 + RR42_RS36020 0.72 -0.1 3,528,626 - RR42_RS36020 0.72 +1.3 3,528,626 - RR42_RS36020 0.72 -0.7 3,528,753 + RR42_RS36020 0.83 -1.3 3,528,754 - RR42_RS36020 0.83 -1.9 3,528,754 - RR42_RS36020 0.83 +0.8 3,529,052 + -0.9 3,529,053 - -1.4 3,529,059 - +0.4 3,529,210 + -0.0 3,529,210 + +0.7 3,529,211 - -1.0 3,529,269 - +0.6 3,529,273 + +0.7 3,529,274 - -3.3 3,529,274 - +0.2 3,529,274 - -0.6 3,529,277 + -0.4 3,529,410 + -0.9 3,529,411 - -0.8 3,529,414 + -1.7 3,529,422 - -1.6 3,529,422 - +0.2 3,529,469 + +0.3 3,529,516 + +0.3 3,529,516 + -1.0 3,529,517 - -0.4 3,529,517 - +0.0 3,529,655 - -1.2 3,529,655 - -2.6 3,529,742 - -1.2 3,529,909 + RR42_RS36025 0.18 -0.7 3,529,909 + RR42_RS36025 0.18 +1.1 3,529,910 - RR42_RS36025 0.18 -1.3 3,529,910 - RR42_RS36025 0.18 -0.1
Or see this region's nucleotide sequence