Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35880 and RR42_RS35885 are separated by 316 nucleotides RR42_RS35885 and RR42_RS35890 are separated by 60 nucleotides RR42_RS35890 and RR42_RS35895 are separated by 129 nucleotides RR42_RS35895 and RR42_RS35900 are separated by 311 nucleotides
RR42_RS35880: RR42_RS35880 - integrase, at 3,498,037 to 3,499,017
_RS35880
RR42_RS35885: RR42_RS35885 - membrane protein, at 3,499,334 to 3,499,771
_RS35885
RR42_RS35890: RR42_RS35890 - 2-nitropropane dioxygenase, at 3,499,832 to 3,500,833
_RS35890
RR42_RS35895: RR42_RS35895 - PadR family transcriptional regulator, at 3,500,963 to 3,501,508
_RS35895
RR42_RS35900: RR42_RS35900 - hypothetical protein, at 3,501,820 to 3,502,041
_RS35900
Position (kb)
3499
3500
3501 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3498.878 kb on + strand, within RR42_RS35880 at 3499.012 kb on - strand at 3499.012 kb on - strand at 3499.051 kb on + strand at 3499.052 kb on - strand at 3499.052 kb on - strand at 3499.056 kb on + strand at 3499.131 kb on + strand at 3499.189 kb on + strand at 3499.189 kb on + strand at 3499.190 kb on - strand at 3499.232 kb on + strand at 3499.232 kb on + strand at 3499.233 kb on - strand at 3499.233 kb on - strand at 3499.233 kb on - strand at 3499.375 kb on - strand at 3499.642 kb on + strand, within RR42_RS35885 at 3499.790 kb on + strand at 3499.790 kb on + strand at 3499.790 kb on + strand at 3499.797 kb on - strand at 3499.810 kb on + strand at 3499.810 kb on + strand at 3499.810 kb on + strand at 3499.811 kb on - strand at 3499.811 kb on - strand at 3499.811 kb on - strand at 3499.811 kb on - strand at 3499.811 kb on - strand at 3500.132 kb on + strand, within RR42_RS35890 at 3500.132 kb on + strand, within RR42_RS35890 at 3500.132 kb on + strand, within RR42_RS35890 at 3500.133 kb on - strand, within RR42_RS35890 at 3500.133 kb on - strand, within RR42_RS35890 at 3500.144 kb on - strand, within RR42_RS35890 at 3500.199 kb on - strand, within RR42_RS35890 at 3500.199 kb on - strand, within RR42_RS35890 at 3500.199 kb on - strand, within RR42_RS35890 at 3500.438 kb on + strand, within RR42_RS35890 at 3500.438 kb on + strand, within RR42_RS35890 at 3500.438 kb on + strand, within RR42_RS35890 at 3500.438 kb on + strand, within RR42_RS35890 at 3500.439 kb on - strand, within RR42_RS35890 at 3500.531 kb on + strand, within RR42_RS35890 at 3500.531 kb on + strand, within RR42_RS35890 at 3500.531 kb on + strand, within RR42_RS35890 at 3500.532 kb on - strand, within RR42_RS35890 at 3500.546 kb on - strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.565 kb on - strand, within RR42_RS35890 at 3500.713 kb on - strand, within RR42_RS35890 at 3500.753 kb on + strand at 3500.754 kb on - strand at 3500.754 kb on - strand at 3500.798 kb on + strand at 3500.799 kb on - strand at 3500.895 kb on + strand at 3500.895 kb on + strand at 3500.983 kb on + strand at 3501.033 kb on + strand, within RR42_RS35895 at 3501.123 kb on + strand, within RR42_RS35895 at 3501.123 kb on + strand, within RR42_RS35895 at 3501.123 kb on + strand, within RR42_RS35895 at 3501.123 kb on + strand, within RR42_RS35895 at 3501.124 kb on - strand, within RR42_RS35895 at 3501.124 kb on - strand, within RR42_RS35895 at 3501.304 kb on - strand, within RR42_RS35895 at 3501.418 kb on - strand, within RR42_RS35895 at 3501.550 kb on + strand at 3501.590 kb on + strand at 3501.697 kb on - strand at 3501.697 kb on - strand at 3501.710 kb on + strand at 3501.711 kb on - strand at 3501.734 kb on - strand at 3501.734 kb on - strand at 3501.734 kb on - strand at 3501.734 kb on - strand at 3501.747 kb on + strand at 3501.747 kb on + strand at 3501.747 kb on + strand at 3501.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,498,878 + RR42_RS35880 0.86 +0.3 3,499,012 - +1.4 3,499,012 - -0.8 3,499,051 + -0.9 3,499,052 - -1.2 3,499,052 - -0.1 3,499,056 + -0.9 3,499,131 + -0.6 3,499,189 + -0.1 3,499,189 + +0.5 3,499,190 - +0.2 3,499,232 + +0.0 3,499,232 + +0.8 3,499,233 - -0.9 3,499,233 - -0.0 3,499,233 - -0.1 3,499,375 - -0.3 3,499,642 + RR42_RS35885 0.70 +2.3 3,499,790 + -1.8 3,499,790 + -0.4 3,499,790 + +1.3 3,499,797 - +0.1 3,499,810 + +1.4 3,499,810 + +0.4 3,499,810 + +0.3 3,499,811 - +0.2 3,499,811 - -1.7 3,499,811 - -0.2 3,499,811 - -0.8 3,499,811 - -0.1 3,500,132 + RR42_RS35890 0.30 -0.4 3,500,132 + RR42_RS35890 0.30 -1.1 3,500,132 + RR42_RS35890 0.30 -0.1 3,500,133 - RR42_RS35890 0.30 -0.0 3,500,133 - RR42_RS35890 0.30 -0.0 3,500,144 - RR42_RS35890 0.31 +1.3 3,500,199 - RR42_RS35890 0.37 +0.7 3,500,199 - RR42_RS35890 0.37 +0.6 3,500,199 - RR42_RS35890 0.37 -0.0 3,500,438 + RR42_RS35890 0.60 +0.6 3,500,438 + RR42_RS35890 0.60 -0.2 3,500,438 + RR42_RS35890 0.60 +0.7 3,500,438 + RR42_RS35890 0.60 +0.5 3,500,439 - RR42_RS35890 0.61 +1.0 3,500,531 + RR42_RS35890 0.70 -1.9 3,500,531 + RR42_RS35890 0.70 -0.2 3,500,531 + RR42_RS35890 0.70 +0.5 3,500,532 - RR42_RS35890 0.70 +0.1 3,500,546 - RR42_RS35890 0.71 +0.5 3,500,564 + RR42_RS35890 0.73 +0.4 3,500,564 + RR42_RS35890 0.73 +0.2 3,500,564 + RR42_RS35890 0.73 +0.3 3,500,564 + RR42_RS35890 0.73 +0.5 3,500,564 + RR42_RS35890 0.73 -0.0 3,500,564 + RR42_RS35890 0.73 -0.9 3,500,565 - RR42_RS35890 0.73 -2.0 3,500,713 - RR42_RS35890 0.88 +0.8 3,500,753 + +0.8 3,500,754 - -1.2 3,500,754 - -0.5 3,500,798 + -0.6 3,500,799 - -0.9 3,500,895 + -0.7 3,500,895 + +0.7 3,500,983 + +0.4 3,501,033 + RR42_RS35895 0.13 +0.8 3,501,123 + RR42_RS35895 0.29 -2.3 3,501,123 + RR42_RS35895 0.29 -0.8 3,501,123 + RR42_RS35895 0.29 -0.5 3,501,123 + RR42_RS35895 0.29 -2.0 3,501,124 - RR42_RS35895 0.29 +0.3 3,501,124 - RR42_RS35895 0.29 -1.3 3,501,304 - RR42_RS35895 0.62 +1.1 3,501,418 - RR42_RS35895 0.83 -2.0 3,501,550 + +0.6 3,501,590 + +1.7 3,501,697 - -1.0 3,501,697 - -1.3 3,501,710 + -0.1 3,501,711 - -3.5 3,501,734 - -2.5 3,501,734 - -1.9 3,501,734 - -3.5 3,501,734 - -3.5 3,501,747 + +0.1 3,501,747 + -2.0 3,501,747 + +0.1 3,501,748 - -2.5
Or see this region's nucleotide sequence