Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35440 and RR42_RS35445 are separated by 84 nucleotides RR42_RS35445 and RR42_RS35450 are separated by 10 nucleotides RR42_RS35450 and RR42_RS35455 are separated by 1 nucleotides RR42_RS35455 and RR42_RS35460 are separated by 62 nucleotides
RR42_RS35440: RR42_RS35440 - MBL fold metallo-hydrolase, at 3,404,268 to 3,405,128
_RS35440
RR42_RS35445: RR42_RS35445 - ArsR family transcriptional regulator, at 3,405,213 to 3,405,536
_RS35445
RR42_RS35450: RR42_RS35450 - hypothetical protein, at 3,405,547 to 3,405,981
_RS35450
RR42_RS35455: RR42_RS35455 - membrane protein, at 3,405,983 to 3,406,414
_RS35455
RR42_RS35460: RR42_RS35460 - membrane protein, at 3,406,477 to 3,407,295
_RS35460
Position (kb)
3405
3406
3407 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3405.019 kb on + strand, within RR42_RS35440 at 3405.020 kb on - strand, within RR42_RS35440 at 3405.020 kb on - strand, within RR42_RS35440 at 3405.068 kb on - strand at 3405.076 kb on + strand at 3405.076 kb on + strand at 3405.076 kb on + strand at 3405.077 kb on - strand at 3405.077 kb on - strand at 3405.077 kb on - strand at 3405.156 kb on + strand at 3405.157 kb on - strand at 3405.328 kb on + strand, within RR42_RS35445 at 3405.328 kb on + strand, within RR42_RS35445 at 3405.462 kb on + strand, within RR42_RS35445 at 3405.463 kb on - strand, within RR42_RS35445 at 3405.463 kb on - strand, within RR42_RS35445 at 3405.534 kb on + strand at 3405.535 kb on - strand at 3405.535 kb on - strand at 3405.546 kb on + strand at 3405.546 kb on + strand at 3405.546 kb on + strand at 3405.547 kb on - strand at 3405.564 kb on + strand at 3405.783 kb on + strand, within RR42_RS35450 at 3405.875 kb on + strand, within RR42_RS35450 at 3405.957 kb on - strand at 3406.066 kb on - strand, within RR42_RS35455 at 3406.131 kb on + strand, within RR42_RS35455 at 3406.131 kb on + strand, within RR42_RS35455 at 3406.131 kb on + strand, within RR42_RS35455 at 3406.132 kb on - strand, within RR42_RS35455 at 3406.191 kb on + strand, within RR42_RS35455 at 3406.191 kb on + strand, within RR42_RS35455 at 3406.221 kb on + strand, within RR42_RS35455 at 3406.222 kb on - strand, within RR42_RS35455 at 3406.412 kb on + strand at 3406.412 kb on + strand at 3406.413 kb on - strand at 3406.413 kb on - strand at 3406.463 kb on + strand at 3406.464 kb on - strand at 3406.487 kb on + strand at 3406.488 kb on - strand at 3406.488 kb on - strand at 3406.574 kb on + strand, within RR42_RS35460 at 3406.575 kb on - strand, within RR42_RS35460 at 3406.584 kb on + strand, within RR42_RS35460 at 3406.745 kb on + strand, within RR42_RS35460 at 3406.787 kb on + strand, within RR42_RS35460 at 3406.787 kb on + strand, within RR42_RS35460 at 3406.788 kb on - strand, within RR42_RS35460 at 3406.800 kb on - strand, within RR42_RS35460 at 3407.032 kb on - strand, within RR42_RS35460 at 3407.052 kb on + strand, within RR42_RS35460 at 3407.052 kb on + strand, within RR42_RS35460 at 3407.053 kb on - strand, within RR42_RS35460 at 3407.053 kb on - strand, within RR42_RS35460 at 3407.053 kb on - strand, within RR42_RS35460 at 3407.079 kb on + strand, within RR42_RS35460 at 3407.079 kb on + strand, within RR42_RS35460 at 3407.080 kb on - strand, within RR42_RS35460 at 3407.207 kb on + strand, within RR42_RS35460 at 3407.298 kb on - strand at 3407.307 kb on + strand at 3407.307 kb on + strand at 3407.307 kb on + strand at 3407.314 kb on + strand at 3407.314 kb on + strand at 3407.314 kb on + strand at 3407.314 kb on + strand at 3407.315 kb on - strand at 3407.320 kb on + strand at 3407.372 kb on + strand at 3407.373 kb on - strand at 3407.373 kb on - strand at 3407.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,405,019 + RR42_RS35440 0.87 -1.2 3,405,020 - RR42_RS35440 0.87 -0.4 3,405,020 - RR42_RS35440 0.87 +0.3 3,405,068 - -1.4 3,405,076 + +0.8 3,405,076 + -0.3 3,405,076 + +2.0 3,405,077 - +1.9 3,405,077 - -0.7 3,405,077 - -0.6 3,405,156 + +0.1 3,405,157 - -0.8 3,405,328 + RR42_RS35445 0.35 +0.8 3,405,328 + RR42_RS35445 0.35 +2.9 3,405,462 + RR42_RS35445 0.77 -0.3 3,405,463 - RR42_RS35445 0.77 +0.8 3,405,463 - RR42_RS35445 0.77 +1.6 3,405,534 + -0.2 3,405,535 - +0.2 3,405,535 - +0.1 3,405,546 + +0.4 3,405,546 + -0.6 3,405,546 + -0.7 3,405,547 - +1.0 3,405,564 + -1.0 3,405,783 + RR42_RS35450 0.54 +1.2 3,405,875 + RR42_RS35450 0.75 -0.8 3,405,957 - -1.8 3,406,066 - RR42_RS35455 0.19 -0.9 3,406,131 + RR42_RS35455 0.34 -0.2 3,406,131 + RR42_RS35455 0.34 +0.9 3,406,131 + RR42_RS35455 0.34 +0.1 3,406,132 - RR42_RS35455 0.34 +0.9 3,406,191 + RR42_RS35455 0.48 -0.3 3,406,191 + RR42_RS35455 0.48 +0.3 3,406,221 + RR42_RS35455 0.55 +0.1 3,406,222 - RR42_RS35455 0.55 +0.2 3,406,412 + +1.1 3,406,412 + -0.0 3,406,413 - -0.2 3,406,413 - -2.1 3,406,463 + -0.6 3,406,464 - +0.1 3,406,487 + +0.3 3,406,488 - +0.3 3,406,488 - +0.4 3,406,574 + RR42_RS35460 0.12 -0.0 3,406,575 - RR42_RS35460 0.12 +1.2 3,406,584 + RR42_RS35460 0.13 +0.1 3,406,745 + RR42_RS35460 0.33 -0.7 3,406,787 + RR42_RS35460 0.38 +0.8 3,406,787 + RR42_RS35460 0.38 -2.9 3,406,788 - RR42_RS35460 0.38 +0.3 3,406,800 - RR42_RS35460 0.39 +0.8 3,407,032 - RR42_RS35460 0.68 +0.4 3,407,052 + RR42_RS35460 0.70 -0.3 3,407,052 + RR42_RS35460 0.70 +0.3 3,407,053 - RR42_RS35460 0.70 -1.0 3,407,053 - RR42_RS35460 0.70 +0.6 3,407,053 - RR42_RS35460 0.70 -0.8 3,407,079 + RR42_RS35460 0.74 +0.3 3,407,079 + RR42_RS35460 0.74 +0.1 3,407,080 - RR42_RS35460 0.74 -0.8 3,407,207 + RR42_RS35460 0.89 -0.3 3,407,298 - -1.1 3,407,307 + -0.7 3,407,307 + -0.8 3,407,307 + -0.3 3,407,314 + +0.2 3,407,314 + -2.9 3,407,314 + -0.3 3,407,314 + +0.9 3,407,315 - +0.4 3,407,320 + +0.6 3,407,372 + -1.1 3,407,373 - +1.2 3,407,373 - +0.4 3,407,396 - +0.5
Or see this region's nucleotide sequence