Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35435 and RR42_RS35440 are separated by 12 nucleotides RR42_RS35440 and RR42_RS35445 are separated by 84 nucleotides RR42_RS35445 and RR42_RS35450 are separated by 10 nucleotides RR42_RS35450 and RR42_RS35455 are separated by 1 nucleotides RR42_RS35455 and RR42_RS35460 are separated by 62 nucleotides
RR42_RS35435: RR42_RS35435 - NAD(FAD)-dependent dehydrogenase, at 3,402,588 to 3,404,255
_RS35435
RR42_RS35440: RR42_RS35440 - MBL fold metallo-hydrolase, at 3,404,268 to 3,405,128
_RS35440
RR42_RS35445: RR42_RS35445 - ArsR family transcriptional regulator, at 3,405,213 to 3,405,536
_RS35445
RR42_RS35450: RR42_RS35450 - hypothetical protein, at 3,405,547 to 3,405,981
_RS35450
RR42_RS35455: RR42_RS35455 - membrane protein, at 3,405,983 to 3,406,414
_RS35455
RR42_RS35460: RR42_RS35460 - membrane protein, at 3,406,477 to 3,407,295
_RS35460
Position (kb)
3405
3406 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3404.260 kb on + strand at 3404.260 kb on + strand at 3404.261 kb on - strand at 3404.261 kb on - strand at 3404.261 kb on - strand at 3404.472 kb on + strand, within RR42_RS35440 at 3404.575 kb on + strand, within RR42_RS35440 at 3404.576 kb on - strand, within RR42_RS35440 at 3404.576 kb on - strand, within RR42_RS35440 at 3404.675 kb on - strand, within RR42_RS35440 at 3404.692 kb on - strand, within RR42_RS35440 at 3404.693 kb on + strand, within RR42_RS35440 at 3404.693 kb on + strand, within RR42_RS35440 at 3404.693 kb on + strand, within RR42_RS35440 at 3404.694 kb on - strand, within RR42_RS35440 at 3404.888 kb on - strand, within RR42_RS35440 at 3404.888 kb on - strand, within RR42_RS35440 at 3405.019 kb on + strand, within RR42_RS35440 at 3405.020 kb on - strand, within RR42_RS35440 at 3405.020 kb on - strand, within RR42_RS35440 at 3405.068 kb on - strand at 3405.076 kb on + strand at 3405.076 kb on + strand at 3405.076 kb on + strand at 3405.077 kb on - strand at 3405.077 kb on - strand at 3405.077 kb on - strand at 3405.156 kb on + strand at 3405.157 kb on - strand at 3405.328 kb on + strand, within RR42_RS35445 at 3405.328 kb on + strand, within RR42_RS35445 at 3405.462 kb on + strand, within RR42_RS35445 at 3405.463 kb on - strand, within RR42_RS35445 at 3405.463 kb on - strand, within RR42_RS35445 at 3405.534 kb on + strand at 3405.535 kb on - strand at 3405.535 kb on - strand at 3405.546 kb on + strand at 3405.546 kb on + strand at 3405.546 kb on + strand at 3405.547 kb on - strand at 3405.564 kb on + strand at 3405.783 kb on + strand, within RR42_RS35450 at 3405.875 kb on + strand, within RR42_RS35450 at 3405.957 kb on - strand at 3406.066 kb on - strand, within RR42_RS35455 at 3406.131 kb on + strand, within RR42_RS35455 at 3406.131 kb on + strand, within RR42_RS35455 at 3406.131 kb on + strand, within RR42_RS35455 at 3406.132 kb on - strand, within RR42_RS35455 at 3406.191 kb on + strand, within RR42_RS35455 at 3406.191 kb on + strand, within RR42_RS35455 at 3406.221 kb on + strand, within RR42_RS35455 at 3406.222 kb on - strand, within RR42_RS35455 at 3406.412 kb on + strand at 3406.412 kb on + strand at 3406.413 kb on - strand at 3406.413 kb on - strand at 3406.463 kb on + strand at 3406.464 kb on - strand at 3406.487 kb on + strand at 3406.488 kb on - strand at 3406.488 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,404,260 + +1.1 3,404,260 + -0.4 3,404,261 - -0.5 3,404,261 - +0.6 3,404,261 - +0.4 3,404,472 + RR42_RS35440 0.24 -0.4 3,404,575 + RR42_RS35440 0.36 +0.6 3,404,576 - RR42_RS35440 0.36 -0.4 3,404,576 - RR42_RS35440 0.36 +2.7 3,404,675 - RR42_RS35440 0.47 +0.1 3,404,692 - RR42_RS35440 0.49 +1.1 3,404,693 + RR42_RS35440 0.49 -0.6 3,404,693 + RR42_RS35440 0.49 -0.2 3,404,693 + RR42_RS35440 0.49 -0.9 3,404,694 - RR42_RS35440 0.49 -1.1 3,404,888 - RR42_RS35440 0.72 +0.9 3,404,888 - RR42_RS35440 0.72 -0.9 3,405,019 + RR42_RS35440 0.87 -1.2 3,405,020 - RR42_RS35440 0.87 -0.4 3,405,020 - RR42_RS35440 0.87 +0.3 3,405,068 - -1.4 3,405,076 + +0.8 3,405,076 + -0.3 3,405,076 + +2.0 3,405,077 - +1.9 3,405,077 - -0.7 3,405,077 - -0.6 3,405,156 + +0.1 3,405,157 - -0.8 3,405,328 + RR42_RS35445 0.35 +0.8 3,405,328 + RR42_RS35445 0.35 +2.9 3,405,462 + RR42_RS35445 0.77 -0.3 3,405,463 - RR42_RS35445 0.77 +0.8 3,405,463 - RR42_RS35445 0.77 +1.6 3,405,534 + -0.2 3,405,535 - +0.2 3,405,535 - +0.1 3,405,546 + +0.4 3,405,546 + -0.6 3,405,546 + -0.7 3,405,547 - +1.0 3,405,564 + -1.0 3,405,783 + RR42_RS35450 0.54 +1.2 3,405,875 + RR42_RS35450 0.75 -0.8 3,405,957 - -1.8 3,406,066 - RR42_RS35455 0.19 -0.9 3,406,131 + RR42_RS35455 0.34 -0.2 3,406,131 + RR42_RS35455 0.34 +0.9 3,406,131 + RR42_RS35455 0.34 +0.1 3,406,132 - RR42_RS35455 0.34 +0.9 3,406,191 + RR42_RS35455 0.48 -0.3 3,406,191 + RR42_RS35455 0.48 +0.3 3,406,221 + RR42_RS35455 0.55 +0.1 3,406,222 - RR42_RS35455 0.55 +0.2 3,406,412 + +1.1 3,406,412 + -0.0 3,406,413 - -0.2 3,406,413 - -2.1 3,406,463 + -0.6 3,406,464 - +0.1 3,406,487 + +0.3 3,406,488 - +0.3 3,406,488 - +0.4
Or see this region's nucleotide sequence