Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35060 and RR42_RS35065 are separated by 399 nucleotides RR42_RS35065 and RR42_RS35070 are separated by 151 nucleotides
RR42_RS35060: RR42_RS35060 - LysR family transcriptional regulator, at 3,318,259 to 3,319,212
_RS35060
RR42_RS35065: RR42_RS35065 - LuxR family transcriptional regulator, at 3,319,612 to 3,320,313
_RS35065
RR42_RS35070: RR42_RS35070 - cobyrinic acid a,c-diamide synthase, at 3,320,465 to 3,321,742
_RS35070
Position (kb)
3319
3320
3321 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3318.836 kb on + strand, within RR42_RS35060 at 3318.836 kb on + strand, within RR42_RS35060 at 3318.836 kb on + strand, within RR42_RS35060 at 3318.837 kb on - strand, within RR42_RS35060 at 3318.837 kb on - strand, within RR42_RS35060 at 3319.201 kb on + strand at 3319.202 kb on - strand at 3319.202 kb on - strand at 3319.312 kb on - strand at 3319.312 kb on - strand at 3319.312 kb on - strand at 3319.342 kb on + strand at 3319.342 kb on + strand at 3319.342 kb on + strand at 3319.343 kb on - strand at 3319.343 kb on - strand at 3319.343 kb on - strand at 3319.565 kb on + strand at 3319.565 kb on + strand at 3319.565 kb on + strand at 3319.565 kb on + strand at 3319.566 kb on - strand at 3319.566 kb on - strand at 3319.566 kb on - strand at 3319.566 kb on - strand at 3319.566 kb on - strand at 3319.566 kb on - strand at 3319.653 kb on + strand at 3319.653 kb on + strand at 3319.654 kb on - strand at 3319.761 kb on + strand, within RR42_RS35065 at 3319.762 kb on - strand, within RR42_RS35065 at 3319.762 kb on - strand, within RR42_RS35065 at 3319.777 kb on + strand, within RR42_RS35065 at 3319.778 kb on - strand, within RR42_RS35065 at 3319.850 kb on + strand, within RR42_RS35065 at 3320.104 kb on + strand, within RR42_RS35065 at 3320.104 kb on + strand, within RR42_RS35065 at 3320.309 kb on + strand at 3320.310 kb on - strand at 3320.310 kb on - strand at 3320.310 kb on - strand at 3320.310 kb on - strand at 3320.311 kb on + strand at 3320.312 kb on - strand at 3320.312 kb on - strand at 3320.364 kb on - strand at 3320.364 kb on - strand at 3320.364 kb on - strand at 3320.364 kb on - strand at 3320.785 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.941 kb on + strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3320.942 kb on - strand, within RR42_RS35070 at 3321.214 kb on + strand, within RR42_RS35070 at 3321.215 kb on - strand, within RR42_RS35070 at 3321.215 kb on - strand, within RR42_RS35070 at 3321.268 kb on + strand, within RR42_RS35070 at 3321.269 kb on - strand, within RR42_RS35070
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,318,836 + RR42_RS35060 0.60 +1.3 3,318,836 + RR42_RS35060 0.60 +0.6 3,318,836 + RR42_RS35060 0.60 +0.1 3,318,837 - RR42_RS35060 0.61 +1.9 3,318,837 - RR42_RS35060 0.61 +1.2 3,319,201 + -0.5 3,319,202 - -1.1 3,319,202 - +1.5 3,319,312 - +0.1 3,319,312 - -1.3 3,319,312 - +0.1 3,319,342 + +0.3 3,319,342 + +1.3 3,319,342 + -0.4 3,319,343 - -1.8 3,319,343 - -0.0 3,319,343 - -0.8 3,319,565 + +1.4 3,319,565 + +0.1 3,319,565 + -0.9 3,319,565 + +1.1 3,319,566 - +0.6 3,319,566 - -1.3 3,319,566 - -0.4 3,319,566 - +0.7 3,319,566 - -3.2 3,319,566 - -1.3 3,319,653 + -1.1 3,319,653 + +0.2 3,319,654 - +0.6 3,319,761 + RR42_RS35065 0.21 +0.6 3,319,762 - RR42_RS35065 0.21 -0.4 3,319,762 - RR42_RS35065 0.21 -1.7 3,319,777 + RR42_RS35065 0.24 +1.1 3,319,778 - RR42_RS35065 0.24 +0.8 3,319,850 + RR42_RS35065 0.34 +0.1 3,320,104 + RR42_RS35065 0.70 -0.4 3,320,104 + RR42_RS35065 0.70 -0.1 3,320,309 + -0.3 3,320,310 - -1.9 3,320,310 - -0.9 3,320,310 - +0.4 3,320,310 - -1.6 3,320,311 + -0.9 3,320,312 - -0.9 3,320,312 - -0.6 3,320,364 - +1.1 3,320,364 - +0.2 3,320,364 - +1.9 3,320,364 - +0.8 3,320,785 + RR42_RS35070 0.25 +1.9 3,320,941 + RR42_RS35070 0.37 -0.3 3,320,941 + RR42_RS35070 0.37 -0.8 3,320,941 + RR42_RS35070 0.37 +1.2 3,320,941 + RR42_RS35070 0.37 -1.2 3,320,941 + RR42_RS35070 0.37 -0.2 3,320,942 - RR42_RS35070 0.37 +0.6 3,320,942 - RR42_RS35070 0.37 -1.2 3,320,942 - RR42_RS35070 0.37 -0.8 3,320,942 - RR42_RS35070 0.37 -0.1 3,320,942 - RR42_RS35070 0.37 +0.6 3,320,942 - RR42_RS35070 0.37 +0.2 3,321,214 + RR42_RS35070 0.59 -0.8 3,321,215 - RR42_RS35070 0.59 +1.9 3,321,215 - RR42_RS35070 0.59 -0.3 3,321,268 + RR42_RS35070 0.63 -0.2 3,321,269 - RR42_RS35070 0.63 -1.5
Or see this region's nucleotide sequence