Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34985 and RR42_RS34990 overlap by 4 nucleotides RR42_RS34990 and RR42_RS34995 are separated by 55 nucleotides RR42_RS34995 and RR42_RS35000 are separated by 152 nucleotides
RR42_RS34985: RR42_RS34985 - mandelate racemase, at 3,300,466 to 3,301,572
_RS34985
RR42_RS34990: RR42_RS34990 - ABC transporter permease, at 3,301,569 to 3,302,972
_RS34990
RR42_RS34995: RR42_RS34995 - ABC transporter permease, at 3,303,028 to 3,303,582
_RS34995
RR42_RS35000: RR42_RS35000 - ABC transporter substrate-binding protein, at 3,303,735 to 3,304,682
_RS35000
Position (kb)
3301
3302
3303 Strain fitness (log2 ratio)
-2
-1
0
1 at 3300.611 kb on + strand, within RR42_RS34985 at 3300.930 kb on - strand, within RR42_RS34985 at 3301.420 kb on - strand, within RR42_RS34985 at 3301.421 kb on + strand, within RR42_RS34985 at 3301.421 kb on + strand, within RR42_RS34985 at 3301.421 kb on + strand, within RR42_RS34985 at 3301.421 kb on + strand, within RR42_RS34985 at 3301.421 kb on + strand, within RR42_RS34985 at 3301.422 kb on - strand, within RR42_RS34985 at 3301.422 kb on - strand, within RR42_RS34985 at 3301.422 kb on - strand, within RR42_RS34985 at 3301.428 kb on - strand, within RR42_RS34985 at 3301.619 kb on - strand at 3301.622 kb on + strand at 3301.623 kb on - strand at 3301.623 kb on - strand at 3301.865 kb on + strand, within RR42_RS34990 at 3302.221 kb on + strand, within RR42_RS34990 at 3302.221 kb on + strand, within RR42_RS34990 at 3302.503 kb on + strand, within RR42_RS34990 at 3302.504 kb on - strand, within RR42_RS34990 at 3302.587 kb on + strand, within RR42_RS34990 at 3302.742 kb on - strand, within RR42_RS34990 at 3302.744 kb on - strand, within RR42_RS34990 at 3302.886 kb on - strand at 3302.886 kb on - strand at 3302.886 kb on - strand at 3302.886 kb on - strand at 3302.887 kb on + strand at 3302.887 kb on + strand at 3302.887 kb on + strand at 3302.887 kb on + strand at 3302.888 kb on - strand at 3302.888 kb on - strand at 3302.888 kb on - strand at 3303.149 kb on + strand, within RR42_RS34995 at 3303.149 kb on + strand, within RR42_RS34995 at 3303.150 kb on - strand, within RR42_RS34995 at 3303.150 kb on - strand, within RR42_RS34995 at 3303.152 kb on + strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.212 kb on + strand, within RR42_RS34995 at 3303.212 kb on + strand, within RR42_RS34995 at 3303.213 kb on - strand, within RR42_RS34995 at 3303.213 kb on - strand, within RR42_RS34995 at 3303.213 kb on - strand, within RR42_RS34995 at 3303.237 kb on - strand, within RR42_RS34995 at 3303.311 kb on + strand, within RR42_RS34995 at 3303.312 kb on - strand, within RR42_RS34995 at 3303.312 kb on - strand, within RR42_RS34995 at 3303.390 kb on - strand, within RR42_RS34995 at 3303.556 kb on - strand at 3303.556 kb on - strand at 3303.556 kb on - strand at 3303.696 kb on + strand at 3303.696 kb on + strand at 3303.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,300,611 + RR42_RS34985 0.13 +0.0 3,300,930 - RR42_RS34985 0.42 +0.1 3,301,420 - RR42_RS34985 0.86 -1.5 3,301,421 + RR42_RS34985 0.86 -0.4 3,301,421 + RR42_RS34985 0.86 -0.3 3,301,421 + RR42_RS34985 0.86 -0.9 3,301,421 + RR42_RS34985 0.86 -0.4 3,301,421 + RR42_RS34985 0.86 -1.1 3,301,422 - RR42_RS34985 0.86 +0.1 3,301,422 - RR42_RS34985 0.86 -0.4 3,301,422 - RR42_RS34985 0.86 +1.3 3,301,428 - RR42_RS34985 0.87 -1.9 3,301,619 - -2.6 3,301,622 + +0.4 3,301,623 - +0.3 3,301,623 - +0.6 3,301,865 + RR42_RS34990 0.21 +1.4 3,302,221 + RR42_RS34990 0.46 +0.9 3,302,221 + RR42_RS34990 0.46 -0.5 3,302,503 + RR42_RS34990 0.67 +0.2 3,302,504 - RR42_RS34990 0.67 -0.4 3,302,587 + RR42_RS34990 0.73 +0.8 3,302,742 - RR42_RS34990 0.84 +1.1 3,302,744 - RR42_RS34990 0.84 -0.5 3,302,886 - -0.5 3,302,886 - -0.2 3,302,886 - +0.5 3,302,886 - -0.8 3,302,887 + +0.9 3,302,887 + -0.9 3,302,887 + -1.8 3,302,887 + +1.2 3,302,888 - -0.3 3,302,888 - +0.9 3,302,888 - +0.1 3,303,149 + RR42_RS34995 0.22 -0.8 3,303,149 + RR42_RS34995 0.22 -1.9 3,303,150 - RR42_RS34995 0.22 +1.5 3,303,150 - RR42_RS34995 0.22 +0.9 3,303,152 + RR42_RS34995 0.22 +0.5 3,303,153 - RR42_RS34995 0.23 -0.1 3,303,153 - RR42_RS34995 0.23 -0.9 3,303,153 - RR42_RS34995 0.23 +0.9 3,303,153 - RR42_RS34995 0.23 -1.9 3,303,153 - RR42_RS34995 0.23 -1.0 3,303,212 + RR42_RS34995 0.33 -0.7 3,303,212 + RR42_RS34995 0.33 -1.4 3,303,213 - RR42_RS34995 0.33 +0.3 3,303,213 - RR42_RS34995 0.33 +0.3 3,303,213 - RR42_RS34995 0.33 +1.5 3,303,237 - RR42_RS34995 0.38 -1.0 3,303,311 + RR42_RS34995 0.51 +0.5 3,303,312 - RR42_RS34995 0.51 -1.3 3,303,312 - RR42_RS34995 0.51 +0.1 3,303,390 - RR42_RS34995 0.65 -0.7 3,303,556 - -0.0 3,303,556 - -0.0 3,303,556 - +0.3 3,303,696 + +0.3 3,303,696 + +0.1 3,303,821 - -0.2
Or see this region's nucleotide sequence