Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33790 and RR42_RS33795 are separated by 124 nucleotides RR42_RS33795 and RR42_RS33800 overlap by 4 nucleotides RR42_RS33800 and RR42_RS33805 are separated by 136 nucleotides
RR42_RS33790: RR42_RS33790 - FAD-dependent oxidoreductase, at 3,010,316 to 3,011,755
_RS33790
RR42_RS33795: RR42_RS33795 - oxidase, at 3,011,880 to 3,013,136
_RS33795
RR42_RS33800: RR42_RS33800 - cytochrome C552, at 3,013,133 to 3,013,807
_RS33800
RR42_RS33805: RR42_RS33805 - 2-aminoadipate aminotransferase, at 3,013,944 to 3,015,458
_RS33805
Position (kb)
3011
3012
3013
3014 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3010.919 kb on + strand, within RR42_RS33790 at 3011.084 kb on + strand, within RR42_RS33790 at 3011.084 kb on + strand, within RR42_RS33790 at 3011.085 kb on - strand, within RR42_RS33790 at 3011.146 kb on + strand, within RR42_RS33790 at 3011.146 kb on + strand, within RR42_RS33790 at 3011.147 kb on - strand, within RR42_RS33790 at 3011.297 kb on + strand, within RR42_RS33790 at 3011.300 kb on + strand, within RR42_RS33790 at 3011.301 kb on - strand, within RR42_RS33790 at 3011.301 kb on - strand, within RR42_RS33790 at 3011.374 kb on + strand, within RR42_RS33790 at 3011.374 kb on + strand, within RR42_RS33790 at 3011.374 kb on + strand, within RR42_RS33790 at 3011.374 kb on + strand, within RR42_RS33790 at 3011.375 kb on - strand, within RR42_RS33790 at 3011.522 kb on + strand, within RR42_RS33790 at 3011.522 kb on + strand, within RR42_RS33790 at 3011.522 kb on + strand, within RR42_RS33790 at 3011.522 kb on + strand, within RR42_RS33790 at 3011.523 kb on - strand, within RR42_RS33790 at 3011.525 kb on + strand, within RR42_RS33790 at 3011.525 kb on + strand, within RR42_RS33790 at 3011.558 kb on + strand, within RR42_RS33790 at 3011.558 kb on + strand, within RR42_RS33790 at 3011.559 kb on - strand, within RR42_RS33790 at 3011.720 kb on + strand at 3011.729 kb on + strand at 3011.826 kb on + strand at 3012.174 kb on + strand, within RR42_RS33795 at 3012.217 kb on - strand, within RR42_RS33795 at 3012.229 kb on + strand, within RR42_RS33795 at 3012.633 kb on + strand, within RR42_RS33795 at 3012.634 kb on - strand, within RR42_RS33795 at 3012.634 kb on - strand, within RR42_RS33795 at 3012.643 kb on - strand, within RR42_RS33795 at 3012.714 kb on + strand, within RR42_RS33795 at 3012.715 kb on - strand, within RR42_RS33795 at 3013.092 kb on + strand at 3013.154 kb on + strand at 3013.202 kb on + strand, within RR42_RS33800 at 3013.202 kb on + strand, within RR42_RS33800 at 3013.337 kb on + strand, within RR42_RS33800 at 3013.337 kb on + strand, within RR42_RS33800 at 3013.337 kb on + strand, within RR42_RS33800 at 3013.338 kb on - strand, within RR42_RS33800 at 3013.338 kb on - strand, within RR42_RS33800 at 3013.338 kb on - strand, within RR42_RS33800 at 3013.338 kb on - strand, within RR42_RS33800 at 3013.469 kb on + strand, within RR42_RS33800 at 3013.470 kb on - strand, within RR42_RS33800 at 3013.470 kb on - strand, within RR42_RS33800 at 3013.470 kb on - strand, within RR42_RS33800 at 3013.491 kb on - strand, within RR42_RS33800
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 3,010,919 + RR42_RS33790 0.42 +0.3 3,011,084 + RR42_RS33790 0.53 +0.6 3,011,084 + RR42_RS33790 0.53 +2.5 3,011,085 - RR42_RS33790 0.53 +0.8 3,011,146 + RR42_RS33790 0.58 -0.2 3,011,146 + RR42_RS33790 0.58 +0.8 3,011,147 - RR42_RS33790 0.58 +0.2 3,011,297 + RR42_RS33790 0.68 -0.0 3,011,300 + RR42_RS33790 0.68 +0.3 3,011,301 - RR42_RS33790 0.68 +0.3 3,011,301 - RR42_RS33790 0.68 -0.1 3,011,374 + RR42_RS33790 0.73 -1.0 3,011,374 + RR42_RS33790 0.73 +1.5 3,011,374 + RR42_RS33790 0.73 +1.5 3,011,374 + RR42_RS33790 0.73 -0.4 3,011,375 - RR42_RS33790 0.74 +1.0 3,011,522 + RR42_RS33790 0.84 +0.8 3,011,522 + RR42_RS33790 0.84 +0.3 3,011,522 + RR42_RS33790 0.84 -1.6 3,011,522 + RR42_RS33790 0.84 +0.1 3,011,523 - RR42_RS33790 0.84 +0.7 3,011,525 + RR42_RS33790 0.84 -0.7 3,011,525 + RR42_RS33790 0.84 +0.1 3,011,558 + RR42_RS33790 0.86 -0.0 3,011,558 + RR42_RS33790 0.86 -1.4 3,011,559 - RR42_RS33790 0.86 +0.4 3,011,720 + -0.1 3,011,729 + -0.2 3,011,826 + -0.1 3,012,174 + RR42_RS33795 0.23 -1.0 3,012,217 - RR42_RS33795 0.27 +0.4 3,012,229 + RR42_RS33795 0.28 -1.6 3,012,633 + RR42_RS33795 0.60 -0.2 3,012,634 - RR42_RS33795 0.60 +0.5 3,012,634 - RR42_RS33795 0.60 -0.6 3,012,643 - RR42_RS33795 0.61 +0.1 3,012,714 + RR42_RS33795 0.66 +0.2 3,012,715 - RR42_RS33795 0.66 -0.4 3,013,092 + +0.0 3,013,154 + -0.9 3,013,202 + RR42_RS33800 0.10 -0.6 3,013,202 + RR42_RS33800 0.10 +0.4 3,013,337 + RR42_RS33800 0.30 -0.6 3,013,337 + RR42_RS33800 0.30 -1.8 3,013,337 + RR42_RS33800 0.30 +0.3 3,013,338 - RR42_RS33800 0.30 +1.5 3,013,338 - RR42_RS33800 0.30 -0.1 3,013,338 - RR42_RS33800 0.30 +1.5 3,013,338 - RR42_RS33800 0.30 -1.4 3,013,469 + RR42_RS33800 0.50 -1.3 3,013,470 - RR42_RS33800 0.50 +0.5 3,013,470 - RR42_RS33800 0.50 +0.4 3,013,470 - RR42_RS33800 0.50 +0.4 3,013,491 - RR42_RS33800 0.53 +1.7
Or see this region's nucleotide sequence