Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS33475

Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS33460 and RR42_RS33465 overlap by 4 nucleotidesRR42_RS33465 and RR42_RS33470 are separated by 75 nucleotidesRR42_RS33470 and RR42_RS33475 are separated by 78 nucleotidesRR42_RS33475 and RR42_RS33480 are separated by 334 nucleotides RR42_RS33460: RR42_RS33460 - glutathione S-transferase, at 2,924,848 to 2,925,933 _RS33460 RR42_RS33465: RR42_RS33465 - ArsR family transcriptional regulator, at 2,925,930 to 2,926,313 _RS33465 RR42_RS33470: RR42_RS33470 - hypothetical protein, at 2,926,389 to 2,926,715 _RS33470 RR42_RS33475: RR42_RS33475 - CopG family transcriptional regulator, at 2,926,794 to 2,926,997 _RS33475 RR42_RS33480: RR42_RS33480 - spermidine synthase, at 2,927,332 to 2,928,147 _RS33480 Position (kb) 2926 2927Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2925.918 kb on + strandat 2925.918 kb on + strandat 2925.919 kb on - strandat 2925.919 kb on - strandat 2925.919 kb on - strandat 2925.919 kb on - strandat 2926.391 kb on - strandat 2926.407 kb on + strandat 2926.407 kb on + strandat 2926.407 kb on + strandat 2926.408 kb on - strandat 2926.408 kb on - strandat 2926.434 kb on - strand, within RR42_RS33470at 2926.585 kb on + strand, within RR42_RS33470at 2926.586 kb on - strand, within RR42_RS33470at 2926.586 kb on - strand, within RR42_RS33470at 2926.586 kb on - strand, within RR42_RS33470at 2926.621 kb on + strand, within RR42_RS33470at 2926.622 kb on - strand, within RR42_RS33470at 2926.622 kb on - strand, within RR42_RS33470at 2926.625 kb on + strand, within RR42_RS33470at 2926.625 kb on + strand, within RR42_RS33470at 2926.648 kb on + strand, within RR42_RS33470at 2926.648 kb on + strand, within RR42_RS33470at 2926.648 kb on + strand, within RR42_RS33470at 2926.649 kb on - strand, within RR42_RS33470at 2926.649 kb on - strand, within RR42_RS33470at 2926.649 kb on - strand, within RR42_RS33470at 2926.649 kb on - strand, within RR42_RS33470at 2926.649 kb on - strand, within RR42_RS33470at 2926.652 kb on + strand, within RR42_RS33470at 2926.652 kb on + strand, within RR42_RS33470at 2926.653 kb on - strand, within RR42_RS33470at 2926.690 kb on + strandat 2926.690 kb on + strandat 2926.767 kb on + strandat 2926.768 kb on - strandat 2926.768 kb on - strandat 2926.849 kb on + strand, within RR42_RS33475at 2926.850 kb on - strand, within RR42_RS33475at 2926.850 kb on - strand, within RR42_RS33475at 2926.850 kb on - strand, within RR42_RS33475at 2926.850 kb on - strand, within RR42_RS33475at 2926.850 kb on - strand, within RR42_RS33475at 2926.850 kb on - strand, within RR42_RS33475at 2926.873 kb on + strand, within RR42_RS33475at 2927.013 kb on - strandat 2927.042 kb on - strandat 2927.134 kb on + strandat 2927.134 kb on + strandat 2927.135 kb on - strandat 2927.135 kb on - strandat 2927.135 kb on - strandat 2927.138 kb on + strandat 2927.146 kb on - strandat 2927.146 kb on - strandat 2927.146 kb on - strandat 2927.346 kb on + strandat 2927.346 kb on + strandat 2927.362 kb on + strandat 2927.363 kb on - strandat 2927.503 kb on + strand, within RR42_RS33480at 2927.593 kb on + strand, within RR42_RS33480at 2927.593 kb on + strand, within RR42_RS33480at 2927.593 kb on + strand, within RR42_RS33480at 2927.593 kb on + strand, within RR42_RS33480at 2927.594 kb on - strand, within RR42_RS33480at 2927.594 kb on - strand, within RR42_RS33480at 2927.594 kb on - strand, within RR42_RS33480at 2927.594 kb on - strand, within RR42_RS33480at 2927.594 kb on - strand, within RR42_RS33480at 2927.594 kb on - strand, within RR42_RS33480at 2927.594 kb on - strand, within RR42_RS33480at 2927.594 kb on - strand, within RR42_RS33480at 2927.594 kb on - strand, within RR42_RS33480at 2927.729 kb on - strand, within RR42_RS33480at 2927.729 kb on - strand, within RR42_RS33480at 2927.729 kb on - strand, within RR42_RS33480at 2927.729 kb on - strand, within RR42_RS33480at 2927.821 kb on + strand, within RR42_RS33480at 2927.858 kb on + strand, within RR42_RS33480at 2927.858 kb on + strand, within RR42_RS33480at 2927.859 kb on - strand, within RR42_RS33480at 2927.872 kb on + strand, within RR42_RS33480at 2927.872 kb on + strand, within RR42_RS33480at 2927.873 kb on - strand, within RR42_RS33480at 2927.892 kb on + strand, within RR42_RS33480at 2927.902 kb on + strand, within RR42_RS33480at 2927.903 kb on - strand, within RR42_RS33480at 2927.971 kb on + strand, within RR42_RS33480at 2927.971 kb on + strand, within RR42_RS33480at 2927.972 kb on - strand, within RR42_RS33480at 2927.972 kb on - strand, within RR42_RS33480

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml
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2,925,918 + +0.1
2,925,918 + -1.8
2,925,919 - -0.2
2,925,919 - -2.1
2,925,919 - -1.4
2,925,919 - -0.5
2,926,391 - +0.6
2,926,407 + +1.0
2,926,407 + +0.5
2,926,407 + +0.8
2,926,408 - +0.8
2,926,408 - +0.3
2,926,434 - RR42_RS33470 0.14 -0.0
2,926,585 + RR42_RS33470 0.60 +0.2
2,926,586 - RR42_RS33470 0.60 +0.6
2,926,586 - RR42_RS33470 0.60 +0.1
2,926,586 - RR42_RS33470 0.60 +2.5
2,926,621 + RR42_RS33470 0.71 +0.2
2,926,622 - RR42_RS33470 0.71 +0.4
2,926,622 - RR42_RS33470 0.71 +0.0
2,926,625 + RR42_RS33470 0.72 +1.6
2,926,625 + RR42_RS33470 0.72 -1.1
2,926,648 + RR42_RS33470 0.79 +1.9
2,926,648 + RR42_RS33470 0.79 +2.1
2,926,648 + RR42_RS33470 0.79 +1.6
2,926,649 - RR42_RS33470 0.80 -1.2
2,926,649 - RR42_RS33470 0.80 -1.2
2,926,649 - RR42_RS33470 0.80 -0.2
2,926,649 - RR42_RS33470 0.80 +0.7
2,926,649 - RR42_RS33470 0.80 -0.3
2,926,652 + RR42_RS33470 0.80 +1.4
2,926,652 + RR42_RS33470 0.80 -0.1
2,926,653 - RR42_RS33470 0.81 -1.2
2,926,690 + -0.3
2,926,690 + +0.5
2,926,767 + +1.0
2,926,768 - -1.5
2,926,768 - -2.3
2,926,849 + RR42_RS33475 0.27 +3.3
2,926,850 - RR42_RS33475 0.27 -1.7
2,926,850 - RR42_RS33475 0.27 -3.0
2,926,850 - RR42_RS33475 0.27 -3.8
2,926,850 - RR42_RS33475 0.27 -2.5
2,926,850 - RR42_RS33475 0.27 -3.6
2,926,850 - RR42_RS33475 0.27 -2.1
2,926,873 + RR42_RS33475 0.39 +1.3
2,927,013 - -0.1
2,927,042 - -4.0
2,927,134 + -0.6
2,927,134 + -0.3
2,927,135 - -1.5
2,927,135 - -2.1
2,927,135 - -2.4
2,927,138 + +1.7
2,927,146 - -3.1
2,927,146 - -3.2
2,927,146 - -4.1
2,927,346 + +0.5
2,927,346 + +0.2
2,927,362 + +0.7
2,927,363 - -0.7
2,927,503 + RR42_RS33480 0.21 +0.2
2,927,593 + RR42_RS33480 0.32 +1.3
2,927,593 + RR42_RS33480 0.32 +0.7
2,927,593 + RR42_RS33480 0.32 +0.4
2,927,593 + RR42_RS33480 0.32 +1.0
2,927,594 - RR42_RS33480 0.32 -3.7
2,927,594 - RR42_RS33480 0.32 -1.1
2,927,594 - RR42_RS33480 0.32 -1.7
2,927,594 - RR42_RS33480 0.32 -1.1
2,927,594 - RR42_RS33480 0.32 -0.7
2,927,594 - RR42_RS33480 0.32 -1.7
2,927,594 - RR42_RS33480 0.32 -4.0
2,927,594 - RR42_RS33480 0.32 -0.5
2,927,594 - RR42_RS33480 0.32 -1.1
2,927,729 - RR42_RS33480 0.49 -1.1
2,927,729 - RR42_RS33480 0.49 -2.5
2,927,729 - RR42_RS33480 0.49 -2.2
2,927,729 - RR42_RS33480 0.49 -2.3
2,927,821 + RR42_RS33480 0.60 +1.2
2,927,858 + RR42_RS33480 0.64 -0.6
2,927,858 + RR42_RS33480 0.64 -0.0
2,927,859 - RR42_RS33480 0.65 -1.0
2,927,872 + RR42_RS33480 0.66 +1.3
2,927,872 + RR42_RS33480 0.66 -1.0
2,927,873 - RR42_RS33480 0.66 -2.3
2,927,892 + RR42_RS33480 0.69 -1.1
2,927,902 + RR42_RS33480 0.70 +1.0
2,927,903 - RR42_RS33480 0.70 +1.4
2,927,971 + RR42_RS33480 0.78 -0.4
2,927,971 + RR42_RS33480 0.78 -0.0
2,927,972 - RR42_RS33480 0.78 +1.3
2,927,972 - RR42_RS33480 0.78 -2.2

Or see this region's nucleotide sequence