Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33170 and RR42_RS33175 are separated by 173 nucleotides RR42_RS33175 and RR42_RS33180 are separated by 494 nucleotides
RR42_RS33170: RR42_RS33170 - hypothetical protein, at 2,846,459 to 2,847,580
_RS33170
RR42_RS33175: RR42_RS33175 - membrane protein, at 2,847,754 to 2,849,463
_RS33175
RR42_RS33180: RR42_RS33180 - hypothetical protein, at 2,849,958 to 2,850,740
_RS33180
Position (kb)
2847
2848
2849
2850 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2846.790 kb on + strand, within RR42_RS33170 at 2846.791 kb on - strand, within RR42_RS33170 at 2846.791 kb on - strand, within RR42_RS33170 at 2846.791 kb on - strand, within RR42_RS33170 at 2846.959 kb on + strand, within RR42_RS33170 at 2847.216 kb on + strand, within RR42_RS33170 at 2847.217 kb on - strand, within RR42_RS33170 at 2847.239 kb on - strand, within RR42_RS33170 at 2847.264 kb on + strand, within RR42_RS33170 at 2847.285 kb on + strand, within RR42_RS33170 at 2847.285 kb on + strand, within RR42_RS33170 at 2847.286 kb on - strand, within RR42_RS33170 at 2847.321 kb on + strand, within RR42_RS33170 at 2847.321 kb on + strand, within RR42_RS33170 at 2847.394 kb on + strand, within RR42_RS33170 at 2847.426 kb on + strand, within RR42_RS33170 at 2847.426 kb on + strand, within RR42_RS33170 at 2847.426 kb on + strand, within RR42_RS33170 at 2847.427 kb on - strand, within RR42_RS33170 at 2847.427 kb on - strand, within RR42_RS33170 at 2847.427 kb on - strand, within RR42_RS33170 at 2847.427 kb on - strand, within RR42_RS33170 at 2847.571 kb on - strand at 2847.571 kb on - strand at 2847.802 kb on - strand at 2848.205 kb on + strand, within RR42_RS33175 at 2848.205 kb on + strand, within RR42_RS33175 at 2848.205 kb on + strand, within RR42_RS33175 at 2848.206 kb on - strand, within RR42_RS33175 at 2848.206 kb on - strand, within RR42_RS33175 at 2848.206 kb on - strand, within RR42_RS33175 at 2848.206 kb on - strand, within RR42_RS33175 at 2848.212 kb on - strand, within RR42_RS33175 at 2848.212 kb on - strand, within RR42_RS33175 at 2848.212 kb on - strand, within RR42_RS33175 at 2849.138 kb on + strand, within RR42_RS33175 at 2849.138 kb on + strand, within RR42_RS33175 at 2849.139 kb on - strand, within RR42_RS33175 at 2849.318 kb on + strand at 2849.318 kb on + strand at 2849.318 kb on + strand at 2849.319 kb on - strand at 2849.319 kb on - strand at 2849.451 kb on + strand at 2849.492 kb on + strand at 2849.492 kb on + strand at 2849.493 kb on - strand at 2849.500 kb on + strand at 2849.501 kb on - strand at 2849.533 kb on - strand at 2849.540 kb on + strand at 2849.540 kb on + strand at 2849.541 kb on - strand at 2849.556 kb on + strand at 2849.557 kb on - strand at 2849.675 kb on + strand at 2849.676 kb on - strand at 2849.676 kb on - strand at 2849.712 kb on + strand at 2849.717 kb on - strand at 2849.742 kb on + strand at 2849.744 kb on + strand at 2849.747 kb on + strand at 2849.783 kb on + strand at 2849.783 kb on + strand at 2849.938 kb on + strand at 2849.952 kb on + strand at 2849.952 kb on + strand at 2849.953 kb on - strand at 2849.953 kb on - strand at 2849.953 kb on - strand at 2850.004 kb on + strand at 2850.004 kb on + strand at 2850.004 kb on + strand at 2850.005 kb on - strand at 2850.005 kb on - strand at 2850.005 kb on - strand at 2850.005 kb on - strand at 2850.252 kb on - strand, within RR42_RS33180 at 2850.410 kb on - strand, within RR42_RS33180 at 2850.427 kb on + strand, within RR42_RS33180 at 2850.428 kb on - strand, within RR42_RS33180 at 2850.428 kb on - strand, within RR42_RS33180 at 2850.428 kb on - strand, within RR42_RS33180 at 2850.428 kb on - strand, within RR42_RS33180
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,846,790 + RR42_RS33170 0.30 -1.0 2,846,791 - RR42_RS33170 0.30 +0.9 2,846,791 - RR42_RS33170 0.30 -2.0 2,846,791 - RR42_RS33170 0.30 -0.1 2,846,959 + RR42_RS33170 0.45 -0.5 2,847,216 + RR42_RS33170 0.67 +0.5 2,847,217 - RR42_RS33170 0.68 -0.3 2,847,239 - RR42_RS33170 0.70 +1.6 2,847,264 + RR42_RS33170 0.72 -1.1 2,847,285 + RR42_RS33170 0.74 -2.0 2,847,285 + RR42_RS33170 0.74 -0.7 2,847,286 - RR42_RS33170 0.74 -0.1 2,847,321 + RR42_RS33170 0.77 +0.8 2,847,321 + RR42_RS33170 0.77 +0.2 2,847,394 + RR42_RS33170 0.83 -0.8 2,847,426 + RR42_RS33170 0.86 -0.1 2,847,426 + RR42_RS33170 0.86 -0.2 2,847,426 + RR42_RS33170 0.86 +0.7 2,847,427 - RR42_RS33170 0.86 -0.1 2,847,427 - RR42_RS33170 0.86 -2.4 2,847,427 - RR42_RS33170 0.86 -3.3 2,847,427 - RR42_RS33170 0.86 +0.2 2,847,571 - -1.1 2,847,571 - -1.7 2,847,802 - -0.0 2,848,205 + RR42_RS33175 0.26 +0.5 2,848,205 + RR42_RS33175 0.26 -0.1 2,848,205 + RR42_RS33175 0.26 -2.1 2,848,206 - RR42_RS33175 0.26 +0.1 2,848,206 - RR42_RS33175 0.26 +0.4 2,848,206 - RR42_RS33175 0.26 +1.2 2,848,206 - RR42_RS33175 0.26 +1.1 2,848,212 - RR42_RS33175 0.27 +0.2 2,848,212 - RR42_RS33175 0.27 -1.9 2,848,212 - RR42_RS33175 0.27 -0.5 2,849,138 + RR42_RS33175 0.81 +0.4 2,849,138 + RR42_RS33175 0.81 +0.9 2,849,139 - RR42_RS33175 0.81 -0.5 2,849,318 + +0.2 2,849,318 + -1.2 2,849,318 + +1.9 2,849,319 - +0.9 2,849,319 - -1.1 2,849,451 + +1.2 2,849,492 + -0.1 2,849,492 + +0.5 2,849,493 - -0.0 2,849,500 + -1.6 2,849,501 - +0.3 2,849,533 - +1.4 2,849,540 + -1.7 2,849,540 + +0.9 2,849,541 - +0.7 2,849,556 + +0.1 2,849,557 - -1.4 2,849,675 + +0.2 2,849,676 - -0.6 2,849,676 - +0.1 2,849,712 + -0.8 2,849,717 - -0.4 2,849,742 + -2.9 2,849,744 + -0.2 2,849,747 + -0.0 2,849,783 + +0.7 2,849,783 + -0.5 2,849,938 + -0.1 2,849,952 + +0.7 2,849,952 + +0.8 2,849,953 - -0.2 2,849,953 - -0.4 2,849,953 - +0.5 2,850,004 + -3.0 2,850,004 + -2.7 2,850,004 + +0.4 2,850,005 - -2.8 2,850,005 - -2.2 2,850,005 - -1.9 2,850,005 - +0.9 2,850,252 - RR42_RS33180 0.38 -1.3 2,850,410 - RR42_RS33180 0.58 -2.5 2,850,427 + RR42_RS33180 0.60 -0.3 2,850,428 - RR42_RS33180 0.60 -1.2 2,850,428 - RR42_RS33180 0.60 -1.0 2,850,428 - RR42_RS33180 0.60 -1.3 2,850,428 - RR42_RS33180 0.60 -1.1
Or see this region's nucleotide sequence