Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32985

Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS32980 and RR42_RS32985 are separated by 58 nucleotidesRR42_RS32985 and RR42_RS32990 are separated by 153 nucleotides RR42_RS32980: RR42_RS32980 - pyruvate dehydrogenase, at 2,798,768 to 2,801,443 _RS32980 RR42_RS32985: RR42_RS32985 - ABC transporter substrate-binding protein, at 2,801,502 to 2,802,491 _RS32985 RR42_RS32990: RR42_RS32990 - 1-aminocyclopropane-1-carboxylate deaminase, at 2,802,645 to 2,803,661 _RS32990 Position (kb) 2801 2802 2803Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2800.661 kb on - strand, within RR42_RS32980at 2800.786 kb on - strand, within RR42_RS32980at 2800.786 kb on - strand, within RR42_RS32980at 2800.806 kb on + strand, within RR42_RS32980at 2800.807 kb on - strand, within RR42_RS32980at 2800.807 kb on - strand, within RR42_RS32980at 2800.873 kb on - strand, within RR42_RS32980at 2800.987 kb on - strand, within RR42_RS32980at 2801.100 kb on + strand, within RR42_RS32980at 2801.100 kb on + strand, within RR42_RS32980at 2801.101 kb on - strand, within RR42_RS32980at 2801.253 kb on + strandat 2801.261 kb on + strandat 2801.476 kb on + strandat 2801.477 kb on - strandat 2801.594 kb on - strandat 2801.594 kb on - strandat 2801.838 kb on - strand, within RR42_RS32985at 2801.924 kb on - strand, within RR42_RS32985at 2801.924 kb on - strand, within RR42_RS32985at 2801.924 kb on - strand, within RR42_RS32985at 2801.930 kb on + strand, within RR42_RS32985at 2801.930 kb on + strand, within RR42_RS32985at 2801.930 kb on + strand, within RR42_RS32985at 2801.930 kb on + strand, within RR42_RS32985at 2801.930 kb on + strand, within RR42_RS32985at 2801.930 kb on + strand, within RR42_RS32985at 2801.931 kb on - strand, within RR42_RS32985at 2801.931 kb on - strand, within RR42_RS32985at 2801.931 kb on - strand, within RR42_RS32985at 2801.931 kb on - strand, within RR42_RS32985at 2802.016 kb on + strand, within RR42_RS32985at 2802.017 kb on - strand, within RR42_RS32985at 2802.212 kb on + strand, within RR42_RS32985at 2802.212 kb on + strand, within RR42_RS32985at 2802.212 kb on + strand, within RR42_RS32985at 2802.213 kb on - strand, within RR42_RS32985at 2802.213 kb on - strand, within RR42_RS32985at 2802.213 kb on - strand, within RR42_RS32985at 2802.213 kb on - strand, within RR42_RS32985at 2802.213 kb on - strand, within RR42_RS32985at 2802.215 kb on - strand, within RR42_RS32985at 2802.215 kb on - strand, within RR42_RS32985at 2802.496 kb on + strandat 2802.668 kb on + strandat 2802.668 kb on + strandat 2802.668 kb on + strandat 2802.669 kb on - strandat 2802.670 kb on + strandat 2802.707 kb on - strandat 2802.707 kb on - strandat 2802.707 kb on - strandat 2802.875 kb on - strand, within RR42_RS32990at 2803.087 kb on + strand, within RR42_RS32990at 2803.088 kb on - strand, within RR42_RS32990at 2803.088 kb on - strand, within RR42_RS32990at 2803.333 kb on + strand, within RR42_RS32990at 2803.333 kb on + strand, within RR42_RS32990at 2803.333 kb on + strand, within RR42_RS32990at 2803.348 kb on + strand, within RR42_RS32990at 2803.348 kb on + strand, within RR42_RS32990

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml
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2,800,661 - RR42_RS32980 0.71 -1.6
2,800,786 - RR42_RS32980 0.75 -1.0
2,800,786 - RR42_RS32980 0.75 -0.2
2,800,806 + RR42_RS32980 0.76 -0.4
2,800,807 - RR42_RS32980 0.76 +0.9
2,800,807 - RR42_RS32980 0.76 +0.2
2,800,873 - RR42_RS32980 0.79 +0.2
2,800,987 - RR42_RS32980 0.83 -0.3
2,801,100 + RR42_RS32980 0.87 -1.9
2,801,100 + RR42_RS32980 0.87 +1.2
2,801,101 - RR42_RS32980 0.87 +0.5
2,801,253 + +0.0
2,801,261 + +0.3
2,801,476 + +0.4
2,801,477 - -1.4
2,801,594 - -2.1
2,801,594 - -0.7
2,801,838 - RR42_RS32985 0.34 -0.9
2,801,924 - RR42_RS32985 0.43 -0.4
2,801,924 - RR42_RS32985 0.43 -0.1
2,801,924 - RR42_RS32985 0.43 +0.8
2,801,930 + RR42_RS32985 0.43 -2.3
2,801,930 + RR42_RS32985 0.43 -1.5
2,801,930 + RR42_RS32985 0.43 +0.4
2,801,930 + RR42_RS32985 0.43 +0.5
2,801,930 + RR42_RS32985 0.43 -1.5
2,801,930 + RR42_RS32985 0.43 -0.0
2,801,931 - RR42_RS32985 0.43 -0.4
2,801,931 - RR42_RS32985 0.43 +0.7
2,801,931 - RR42_RS32985 0.43 +0.1
2,801,931 - RR42_RS32985 0.43 +0.2
2,802,016 + RR42_RS32985 0.52 +0.2
2,802,017 - RR42_RS32985 0.52 -0.1
2,802,212 + RR42_RS32985 0.72 +0.9
2,802,212 + RR42_RS32985 0.72 +0.9
2,802,212 + RR42_RS32985 0.72 +0.3
2,802,213 - RR42_RS32985 0.72 +0.2
2,802,213 - RR42_RS32985 0.72 +0.7
2,802,213 - RR42_RS32985 0.72 +0.0
2,802,213 - RR42_RS32985 0.72 -0.9
2,802,213 - RR42_RS32985 0.72 -0.1
2,802,215 - RR42_RS32985 0.72 -0.1
2,802,215 - RR42_RS32985 0.72 +0.2
2,802,496 + -1.7
2,802,668 + +0.2
2,802,668 + -0.9
2,802,668 + +0.9
2,802,669 - +1.2
2,802,670 + -1.7
2,802,707 - +0.9
2,802,707 - -0.6
2,802,707 - -0.7
2,802,875 - RR42_RS32990 0.23 +2.6
2,803,087 + RR42_RS32990 0.43 -3.8
2,803,088 - RR42_RS32990 0.44 -1.5
2,803,088 - RR42_RS32990 0.44 +0.4
2,803,333 + RR42_RS32990 0.68 -1.4
2,803,333 + RR42_RS32990 0.68 -2.1
2,803,333 + RR42_RS32990 0.68 -3.1
2,803,348 + RR42_RS32990 0.69 -3.6
2,803,348 + RR42_RS32990 0.69 -3.2

Or see this region's nucleotide sequence