Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32475 and RR42_RS32480 are separated by 24 nucleotides RR42_RS32480 and RR42_RS32485 are separated by 131 nucleotides
RR42_RS32475: RR42_RS32475 - permease, at 2,683,242 to 2,684,201
_RS32475
RR42_RS32480: RR42_RS32480 - 4-hydroxyphenylpyruvate dioxygenase, at 2,684,226 to 2,684,993
_RS32480
RR42_RS32485: RR42_RS32485 - 3-keto-5-aminohexanoate cleavage protein, at 2,685,125 to 2,687,008
_RS32485
Position (kb)
2684
2685 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2683.275 kb on + strand at 2683.276 kb on - strand at 2683.276 kb on - strand at 2683.384 kb on + strand, within RR42_RS32475 at 2683.446 kb on + strand, within RR42_RS32475 at 2683.447 kb on - strand, within RR42_RS32475 at 2683.447 kb on - strand, within RR42_RS32475 at 2683.482 kb on + strand, within RR42_RS32475 at 2683.533 kb on + strand at 2683.533 kb on + strand, within RR42_RS32475 at 2683.534 kb on - strand, within RR42_RS32475 at 2683.833 kb on - strand, within RR42_RS32475 at 2683.966 kb on + strand, within RR42_RS32475 at 2684.070 kb on + strand, within RR42_RS32475 at 2684.071 kb on - strand, within RR42_RS32475 at 2684.092 kb on - strand, within RR42_RS32475 at 2684.319 kb on - strand, within RR42_RS32480 at 2684.417 kb on - strand, within RR42_RS32480 at 2684.485 kb on + strand, within RR42_RS32480 at 2684.485 kb on + strand, within RR42_RS32480 at 2684.593 kb on - strand, within RR42_RS32480 at 2684.644 kb on - strand, within RR42_RS32480 at 2684.675 kb on - strand, within RR42_RS32480 at 2684.752 kb on + strand, within RR42_RS32480 at 2684.843 kb on - strand, within RR42_RS32480 at 2685.078 kb on + strand at 2685.078 kb on + strand at 2685.079 kb on - strand at 2685.090 kb on + strand at 2685.303 kb on + strand at 2685.304 kb on - strand at 2685.304 kb on - strand at 2685.304 kb on - strand at 2685.323 kb on + strand, within RR42_RS32485 at 2685.406 kb on - strand, within RR42_RS32485 at 2685.542 kb on + strand, within RR42_RS32485 at 2685.542 kb on + strand, within RR42_RS32485 at 2685.543 kb on - strand, within RR42_RS32485 at 2685.543 kb on - strand, within RR42_RS32485 at 2685.543 kb on - strand, within RR42_RS32485 at 2685.640 kb on + strand, within RR42_RS32485 at 2685.640 kb on + strand, within RR42_RS32485
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,683,275 + +1.4 2,683,276 - +1.2 2,683,276 - +0.9 2,683,384 + RR42_RS32475 0.15 -0.9 2,683,446 + RR42_RS32475 0.21 -0.9 2,683,447 - RR42_RS32475 0.21 -2.3 2,683,447 - RR42_RS32475 0.21 +0.9 2,683,482 + RR42_RS32475 0.25 +0.3 2,683,533 + -0.1 2,683,533 + RR42_RS32475 0.30 +0.6 2,683,534 - RR42_RS32475 0.30 -0.2 2,683,833 - RR42_RS32475 0.62 -0.1 2,683,966 + RR42_RS32475 0.75 -0.9 2,684,070 + RR42_RS32475 0.86 -0.7 2,684,071 - RR42_RS32475 0.86 -0.1 2,684,092 - RR42_RS32475 0.89 +1.2 2,684,319 - RR42_RS32480 0.12 +1.1 2,684,417 - RR42_RS32480 0.25 -0.1 2,684,485 + RR42_RS32480 0.34 -0.1 2,684,485 + RR42_RS32480 0.34 +0.0 2,684,593 - RR42_RS32480 0.48 +1.9 2,684,644 - RR42_RS32480 0.54 +0.7 2,684,675 - RR42_RS32480 0.58 +0.0 2,684,752 + RR42_RS32480 0.68 +1.0 2,684,843 - RR42_RS32480 0.80 +0.7 2,685,078 + +0.9 2,685,078 + +0.5 2,685,079 - -0.4 2,685,090 + -0.0 2,685,303 + +4.5 2,685,304 - +0.1 2,685,304 - +1.1 2,685,304 - +0.5 2,685,323 + RR42_RS32485 0.11 +0.2 2,685,406 - RR42_RS32485 0.15 -1.0 2,685,542 + RR42_RS32485 0.22 +0.5 2,685,542 + RR42_RS32485 0.22 +0.6 2,685,543 - RR42_RS32485 0.22 +0.5 2,685,543 - RR42_RS32485 0.22 -1.1 2,685,543 - RR42_RS32485 0.22 +1.6 2,685,640 + RR42_RS32485 0.27 +1.1 2,685,640 + RR42_RS32485 0.27 +0.5
Or see this region's nucleotide sequence