Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32265

Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS32260 and RR42_RS32265 are separated by 93 nucleotidesRR42_RS32265 and RR42_RS32270 are separated by 253 nucleotidesRR42_RS32270 and RR42_RS32275 are separated by 144 nucleotides RR42_RS32260: RR42_RS32260 - squalene--hopene cyclase, at 2,625,018 to 2,627,033 _RS32260 RR42_RS32265: RR42_RS32265 - phytoene synthase, at 2,627,127 to 2,627,975 _RS32265 RR42_RS32270: RR42_RS32270 - hypothetical protein, at 2,628,229 to 2,628,489 _RS32270 RR42_RS32275: RR42_RS32275 - gamma-glutamyltransferase, at 2,628,634 to 2,630,226 _RS32275 Position (kb) 2627 2628Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2626.287 kb on + strand, within RR42_RS32260at 2626.492 kb on + strand, within RR42_RS32260at 2626.492 kb on + strandat 2626.492 kb on + strand, within RR42_RS32260at 2626.493 kb on - strand, within RR42_RS32260at 2626.493 kb on - strand, within RR42_RS32260at 2626.493 kb on - strand, within RR42_RS32260at 2626.493 kb on - strand, within RR42_RS32260at 2626.608 kb on - strand, within RR42_RS32260at 2626.608 kb on - strand, within RR42_RS32260at 2626.711 kb on + strand, within RR42_RS32260at 2626.711 kb on + strand, within RR42_RS32260at 2626.712 kb on - strand, within RR42_RS32260at 2626.806 kb on - strand, within RR42_RS32260at 2626.843 kb on + strandat 2626.844 kb on - strandat 2626.844 kb on - strandat 2626.861 kb on + strandat 2626.861 kb on + strandat 2627.016 kb on + strandat 2627.066 kb on + strandat 2627.067 kb on - strandat 2627.157 kb on + strandat 2627.240 kb on - strand, within RR42_RS32265at 2627.314 kb on + strand, within RR42_RS32265at 2627.315 kb on - strand, within RR42_RS32265at 2627.317 kb on + strand, within RR42_RS32265at 2627.317 kb on + strand, within RR42_RS32265at 2627.318 kb on - strand, within RR42_RS32265at 2627.434 kb on + strand, within RR42_RS32265at 2627.435 kb on - strand, within RR42_RS32265at 2627.435 kb on - strand, within RR42_RS32265at 2627.521 kb on - strand, within RR42_RS32265at 2627.593 kb on + strand, within RR42_RS32265at 2627.593 kb on + strand, within RR42_RS32265at 2627.594 kb on - strand, within RR42_RS32265at 2627.594 kb on - strand, within RR42_RS32265at 2627.594 kb on - strand, within RR42_RS32265at 2627.601 kb on + strand, within RR42_RS32265at 2627.601 kb on + strand, within RR42_RS32265at 2627.601 kb on + strand, within RR42_RS32265at 2627.602 kb on - strand, within RR42_RS32265at 2627.607 kb on - strand, within RR42_RS32265at 2627.746 kb on + strand, within RR42_RS32265at 2627.747 kb on - strand, within RR42_RS32265at 2627.747 kb on - strand, within RR42_RS32265at 2627.822 kb on + strand, within RR42_RS32265at 2627.822 kb on + strand, within RR42_RS32265at 2627.823 kb on - strand, within RR42_RS32265at 2627.823 kb on - strand, within RR42_RS32265at 2627.848 kb on + strand, within RR42_RS32265at 2627.848 kb on + strand, within RR42_RS32265at 2627.848 kb on + strand, within RR42_RS32265at 2627.848 kb on + strand, within RR42_RS32265at 2627.849 kb on - strand, within RR42_RS32265at 2627.849 kb on - strand, within RR42_RS32265at 2627.849 kb on - strand, within RR42_RS32265at 2627.900 kb on + strandat 2627.977 kb on + strandat 2627.977 kb on + strandat 2627.978 kb on - strandat 2627.978 kb on - strandat 2628.021 kb on - strandat 2628.021 kb on - strandat 2628.021 kb on - strandat 2628.086 kb on + strandat 2628.086 kb on + strandat 2628.088 kb on + strandat 2628.088 kb on + strandat 2628.088 kb on + strandat 2628.088 kb on + strandat 2628.088 kb on + strandat 2628.088 kb on + strandat 2628.088 kb on + strandat 2628.089 kb on - strandat 2628.089 kb on - strandat 2628.089 kb on - strandat 2628.089 kb on - strandat 2628.230 kb on + strandat 2628.231 kb on - strandat 2628.234 kb on + strandat 2628.290 kb on + strand, within RR42_RS32270at 2628.308 kb on + strand, within RR42_RS32270at 2628.478 kb on + strandat 2628.478 kb on + strandat 2628.478 kb on + strandat 2628.479 kb on - strandat 2628.479 kb on - strandat 2628.521 kb on + strandat 2628.532 kb on + strandat 2628.532 kb on + strandat 2628.532 kb on + strandat 2628.532 kb on + strandat 2628.532 kb on + strandat 2628.542 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml
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2,626,287 + RR42_RS32260 0.63 -3.1
2,626,492 + RR42_RS32260 0.73 -2.7
2,626,492 + -0.7
2,626,492 + RR42_RS32260 0.73 -1.5
2,626,493 - RR42_RS32260 0.73 -1.3
2,626,493 - RR42_RS32260 0.73 -3.1
2,626,493 - RR42_RS32260 0.73 -4.6
2,626,493 - RR42_RS32260 0.73 -0.8
2,626,608 - RR42_RS32260 0.79 -1.5
2,626,608 - RR42_RS32260 0.79 -3.7
2,626,711 + RR42_RS32260 0.84 -2.8
2,626,711 + RR42_RS32260 0.84 -3.3
2,626,712 - RR42_RS32260 0.84 +0.5
2,626,806 - RR42_RS32260 0.89 -3.6
2,626,843 + -2.4
2,626,844 - -3.8
2,626,844 - -0.9
2,626,861 + -1.1
2,626,861 + -3.7
2,627,016 + +0.4
2,627,066 + -3.3
2,627,067 - +1.9
2,627,157 + -2.0
2,627,240 - RR42_RS32265 0.13 +2.6
2,627,314 + RR42_RS32265 0.22 +1.2
2,627,315 - RR42_RS32265 0.22 +2.1
2,627,317 + RR42_RS32265 0.22 +0.9
2,627,317 + RR42_RS32265 0.22 +1.4
2,627,318 - RR42_RS32265 0.22 +2.4
2,627,434 + RR42_RS32265 0.36 -2.0
2,627,435 - RR42_RS32265 0.36 +2.1
2,627,435 - RR42_RS32265 0.36 +2.8
2,627,521 - RR42_RS32265 0.46 +2.1
2,627,593 + RR42_RS32265 0.55 -0.5
2,627,593 + RR42_RS32265 0.55 -0.3
2,627,594 - RR42_RS32265 0.55 +2.8
2,627,594 - RR42_RS32265 0.55 +3.3
2,627,594 - RR42_RS32265 0.55 +2.0
2,627,601 + RR42_RS32265 0.56 -0.4
2,627,601 + RR42_RS32265 0.56 -2.0
2,627,601 + RR42_RS32265 0.56 +0.5
2,627,602 - RR42_RS32265 0.56 +2.1
2,627,607 - RR42_RS32265 0.57 +2.3
2,627,746 + RR42_RS32265 0.73 -0.3
2,627,747 - RR42_RS32265 0.73 +2.7
2,627,747 - RR42_RS32265 0.73 +1.0
2,627,822 + RR42_RS32265 0.82 -2.1
2,627,822 + RR42_RS32265 0.82 -2.5
2,627,823 - RR42_RS32265 0.82 +2.9
2,627,823 - RR42_RS32265 0.82 +2.6
2,627,848 + RR42_RS32265 0.85 -0.5
2,627,848 + RR42_RS32265 0.85 +0.0
2,627,848 + RR42_RS32265 0.85 -1.1
2,627,848 + RR42_RS32265 0.85 -0.4
2,627,849 - RR42_RS32265 0.85 +2.4
2,627,849 - RR42_RS32265 0.85 +3.3
2,627,849 - RR42_RS32265 0.85 +2.7
2,627,900 + -1.0
2,627,977 + -1.3
2,627,977 + -0.6
2,627,978 - +0.7
2,627,978 - +0.9
2,628,021 - +0.9
2,628,021 - +0.9
2,628,021 - +1.9
2,628,086 + +1.1
2,628,086 + -2.5
2,628,088 + -0.1
2,628,088 + -0.1
2,628,088 + -1.9
2,628,088 + -1.1
2,628,088 + -1.6
2,628,088 + +0.4
2,628,088 + +0.3
2,628,089 - -0.7
2,628,089 - -1.1
2,628,089 - -0.2
2,628,089 - +0.8
2,628,230 + -1.1
2,628,231 - +0.4
2,628,234 + -0.1
2,628,290 + RR42_RS32270 0.23 -0.1
2,628,308 + RR42_RS32270 0.30 -0.9
2,628,478 + -0.3
2,628,478 + -0.1
2,628,478 + +0.3
2,628,479 - +0.4
2,628,479 - +0.7
2,628,521 + +0.1
2,628,532 + -0.1
2,628,532 + +0.6
2,628,532 + -0.9
2,628,532 + +0.1
2,628,532 + -1.5
2,628,542 + -0.3

Or see this region's nucleotide sequence