Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32250 and RR42_RS32255 are separated by 29 nucleotides RR42_RS32255 and RR42_RS32260 are separated by 53 nucleotides RR42_RS32260 and RR42_RS32265 are separated by 93 nucleotides
RR42_RS32250: RR42_RS32250 - ABC transporter, at 2,623,493 to 2,624,296
_RS32250
RR42_RS32255: RR42_RS32255 - squalene-hopene cyclase, at 2,624,326 to 2,624,964
_RS32255
RR42_RS32260: RR42_RS32260 - squalene--hopene cyclase, at 2,625,018 to 2,627,033
_RS32260
RR42_RS32265: RR42_RS32265 - phytoene synthase, at 2,627,127 to 2,627,975
_RS32265
Position (kb)
2625
2626
2627
2628 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.094 kb on - strand, within RR42_RS32250 at 2624.114 kb on + strand, within RR42_RS32250 at 2624.115 kb on - strand, within RR42_RS32250 at 2624.132 kb on + strand, within RR42_RS32250 at 2624.132 kb on + strand, within RR42_RS32250 at 2624.133 kb on - strand, within RR42_RS32250 at 2625.121 kb on + strand at 2625.199 kb on + strand at 2625.200 kb on - strand at 2625.200 kb on - strand at 2625.301 kb on + strand, within RR42_RS32260 at 2625.301 kb on + strand, within RR42_RS32260 at 2625.302 kb on - strand, within RR42_RS32260 at 2625.322 kb on + strand, within RR42_RS32260 at 2625.457 kb on + strand, within RR42_RS32260 at 2625.457 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.464 kb on - strand, within RR42_RS32260 at 2625.464 kb on - strand, within RR42_RS32260 at 2625.464 kb on - strand, within RR42_RS32260 at 2625.464 kb on - strand, within RR42_RS32260 at 2625.484 kb on + strand, within RR42_RS32260 at 2625.484 kb on + strand, within RR42_RS32260 at 2625.650 kb on + strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.878 kb on - strand, within RR42_RS32260 at 2626.287 kb on + strand, within RR42_RS32260 at 2626.492 kb on + strand, within RR42_RS32260 at 2626.492 kb on + strand at 2626.492 kb on + strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.608 kb on - strand, within RR42_RS32260 at 2626.608 kb on - strand, within RR42_RS32260 at 2626.711 kb on + strand, within RR42_RS32260 at 2626.711 kb on + strand, within RR42_RS32260 at 2626.712 kb on - strand, within RR42_RS32260 at 2626.806 kb on - strand, within RR42_RS32260 at 2626.843 kb on + strand at 2626.844 kb on - strand at 2626.844 kb on - strand at 2626.861 kb on + strand at 2626.861 kb on + strand at 2627.016 kb on + strand at 2627.066 kb on + strand at 2627.067 kb on - strand at 2627.157 kb on + strand at 2627.240 kb on - strand, within RR42_RS32265 at 2627.314 kb on + strand, within RR42_RS32265 at 2627.315 kb on - strand, within RR42_RS32265 at 2627.317 kb on + strand, within RR42_RS32265 at 2627.317 kb on + strand, within RR42_RS32265 at 2627.318 kb on - strand, within RR42_RS32265 at 2627.434 kb on + strand, within RR42_RS32265 at 2627.435 kb on - strand, within RR42_RS32265 at 2627.435 kb on - strand, within RR42_RS32265 at 2627.521 kb on - strand, within RR42_RS32265 at 2627.593 kb on + strand, within RR42_RS32265 at 2627.593 kb on + strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.602 kb on - strand, within RR42_RS32265 at 2627.607 kb on - strand, within RR42_RS32265 at 2627.746 kb on + strand, within RR42_RS32265 at 2627.747 kb on - strand, within RR42_RS32265 at 2627.747 kb on - strand, within RR42_RS32265 at 2627.822 kb on + strand, within RR42_RS32265 at 2627.822 kb on + strand, within RR42_RS32265 at 2627.823 kb on - strand, within RR42_RS32265 at 2627.823 kb on - strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.900 kb on + strand at 2627.977 kb on + strand at 2627.977 kb on + strand at 2627.978 kb on - strand at 2627.978 kb on - strand at 2628.021 kb on - strand at 2628.021 kb on - strand at 2628.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,624,090 - RR42_RS32250 0.74 -2.3 2,624,090 - RR42_RS32250 0.74 -2.2 2,624,090 - RR42_RS32250 0.74 -4.0 2,624,090 - RR42_RS32250 0.74 -3.2 2,624,090 - RR42_RS32250 0.74 -4.4 2,624,093 + RR42_RS32250 0.75 -2.6 2,624,093 + RR42_RS32250 0.75 -3.8 2,624,093 + RR42_RS32250 0.75 -3.8 2,624,093 + RR42_RS32250 0.75 -4.4 2,624,093 + RR42_RS32250 0.75 -4.5 2,624,093 + RR42_RS32250 0.75 -4.1 2,624,093 + RR42_RS32250 0.75 -3.8 2,624,094 - RR42_RS32250 0.75 -4.9 2,624,114 + RR42_RS32250 0.77 -3.1 2,624,115 - RR42_RS32250 0.77 -4.7 2,624,132 + RR42_RS32250 0.79 -3.3 2,624,132 + RR42_RS32250 0.79 -3.5 2,624,133 - RR42_RS32250 0.80 -2.1 2,625,121 + +0.9 2,625,199 + -0.7 2,625,200 - -0.8 2,625,200 - -1.4 2,625,301 + RR42_RS32260 0.14 -3.1 2,625,301 + RR42_RS32260 0.14 -2.6 2,625,302 - RR42_RS32260 0.14 -3.3 2,625,322 + RR42_RS32260 0.15 -2.5 2,625,457 + RR42_RS32260 0.22 -0.1 2,625,457 + RR42_RS32260 0.22 -3.1 2,625,463 + RR42_RS32260 0.22 -2.7 2,625,463 + RR42_RS32260 0.22 -2.3 2,625,463 + RR42_RS32260 0.22 -2.3 2,625,463 + RR42_RS32260 0.22 -3.0 2,625,463 + RR42_RS32260 0.22 -3.8 2,625,463 + RR42_RS32260 0.22 -4.0 2,625,464 - RR42_RS32260 0.22 -2.1 2,625,464 - RR42_RS32260 0.22 +0.3 2,625,464 - RR42_RS32260 0.22 -4.5 2,625,464 - RR42_RS32260 0.22 -3.2 2,625,484 + RR42_RS32260 0.23 -1.1 2,625,484 + RR42_RS32260 0.23 -1.9 2,625,650 + RR42_RS32260 0.31 -2.8 2,625,662 - RR42_RS32260 0.32 -0.8 2,625,662 - RR42_RS32260 0.32 -2.7 2,625,662 - RR42_RS32260 0.32 -2.3 2,625,662 - RR42_RS32260 0.32 -1.4 2,625,662 - RR42_RS32260 0.32 -1.4 2,625,878 - RR42_RS32260 0.43 -3.8 2,626,287 + RR42_RS32260 0.63 -3.1 2,626,492 + RR42_RS32260 0.73 -2.7 2,626,492 + -0.7 2,626,492 + RR42_RS32260 0.73 -1.5 2,626,493 - RR42_RS32260 0.73 -1.3 2,626,493 - RR42_RS32260 0.73 -3.1 2,626,493 - RR42_RS32260 0.73 -4.6 2,626,493 - RR42_RS32260 0.73 -0.8 2,626,608 - RR42_RS32260 0.79 -1.5 2,626,608 - RR42_RS32260 0.79 -3.7 2,626,711 + RR42_RS32260 0.84 -2.8 2,626,711 + RR42_RS32260 0.84 -3.3 2,626,712 - RR42_RS32260 0.84 +0.5 2,626,806 - RR42_RS32260 0.89 -3.6 2,626,843 + -2.4 2,626,844 - -3.8 2,626,844 - -0.9 2,626,861 + -1.1 2,626,861 + -3.7 2,627,016 + +0.4 2,627,066 + -3.3 2,627,067 - +1.9 2,627,157 + -2.0 2,627,240 - RR42_RS32265 0.13 +2.6 2,627,314 + RR42_RS32265 0.22 +1.2 2,627,315 - RR42_RS32265 0.22 +2.1 2,627,317 + RR42_RS32265 0.22 +0.9 2,627,317 + RR42_RS32265 0.22 +1.4 2,627,318 - RR42_RS32265 0.22 +2.4 2,627,434 + RR42_RS32265 0.36 -2.0 2,627,435 - RR42_RS32265 0.36 +2.1 2,627,435 - RR42_RS32265 0.36 +2.8 2,627,521 - RR42_RS32265 0.46 +2.1 2,627,593 + RR42_RS32265 0.55 -0.5 2,627,593 + RR42_RS32265 0.55 -0.3 2,627,594 - RR42_RS32265 0.55 +2.8 2,627,594 - RR42_RS32265 0.55 +3.3 2,627,594 - RR42_RS32265 0.55 +2.0 2,627,601 + RR42_RS32265 0.56 -0.4 2,627,601 + RR42_RS32265 0.56 -2.0 2,627,601 + RR42_RS32265 0.56 +0.5 2,627,602 - RR42_RS32265 0.56 +2.1 2,627,607 - RR42_RS32265 0.57 +2.3 2,627,746 + RR42_RS32265 0.73 -0.3 2,627,747 - RR42_RS32265 0.73 +2.7 2,627,747 - RR42_RS32265 0.73 +1.0 2,627,822 + RR42_RS32265 0.82 -2.1 2,627,822 + RR42_RS32265 0.82 -2.5 2,627,823 - RR42_RS32265 0.82 +2.9 2,627,823 - RR42_RS32265 0.82 +2.6 2,627,848 + RR42_RS32265 0.85 -0.5 2,627,848 + RR42_RS32265 0.85 +0.0 2,627,848 + RR42_RS32265 0.85 -1.1 2,627,848 + RR42_RS32265 0.85 -0.4 2,627,849 - RR42_RS32265 0.85 +2.4 2,627,849 - RR42_RS32265 0.85 +3.3 2,627,849 - RR42_RS32265 0.85 +2.7 2,627,900 + -1.0 2,627,977 + -1.3 2,627,977 + -0.6 2,627,978 - +0.7 2,627,978 - +0.9 2,628,021 - +0.9 2,628,021 - +0.9 2,628,021 - +1.9
Or see this region's nucleotide sequence