Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32245 and RR42_RS32250 are separated by 62 nucleotides RR42_RS32250 and RR42_RS32255 are separated by 29 nucleotides RR42_RS32255 and RR42_RS32260 are separated by 53 nucleotides
RR42_RS32245: RR42_RS32245 - RND transporter, at 2,620,761 to 2,623,430
_RS32245
RR42_RS32250: RR42_RS32250 - ABC transporter, at 2,623,493 to 2,624,296
_RS32250
RR42_RS32255: RR42_RS32255 - squalene-hopene cyclase, at 2,624,326 to 2,624,964
_RS32255
RR42_RS32260: RR42_RS32260 - squalene--hopene cyclase, at 2,625,018 to 2,627,033
_RS32260
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2622.875 kb on + strand, within RR42_RS32245 at 2622.876 kb on - strand, within RR42_RS32245 at 2622.876 kb on - strand, within RR42_RS32245 at 2622.876 kb on - strand, within RR42_RS32245 at 2623.173 kb on + strand at 2623.173 kb on + strand at 2623.173 kb on + strand at 2623.173 kb on + strand at 2623.174 kb on - strand at 2623.174 kb on - strand at 2623.174 kb on - strand at 2623.174 kb on - strand at 2623.174 kb on - strand at 2623.174 kb on - strand at 2623.174 kb on - strand at 2623.174 kb on - strand at 2623.176 kb on + strand at 2623.176 kb on + strand at 2623.176 kb on + strand at 2623.177 kb on - strand at 2623.177 kb on - strand at 2623.177 kb on - strand at 2623.177 kb on - strand at 2623.177 kb on - strand at 2623.177 kb on - strand at 2623.237 kb on + strand at 2623.238 kb on - strand at 2623.361 kb on - strand at 2623.444 kb on + strand at 2623.444 kb on + strand at 2623.445 kb on - strand at 2623.445 kb on - strand at 2623.445 kb on - strand at 2623.445 kb on - strand at 2623.616 kb on + strand, within RR42_RS32250 at 2623.616 kb on + strand, within RR42_RS32250 at 2623.617 kb on - strand, within RR42_RS32250 at 2623.617 kb on - strand, within RR42_RS32250 at 2623.692 kb on - strand, within RR42_RS32250 at 2623.718 kb on - strand, within RR42_RS32250 at 2623.718 kb on - strand, within RR42_RS32250 at 2623.898 kb on + strand, within RR42_RS32250 at 2623.952 kb on + strand, within RR42_RS32250 at 2623.952 kb on + strand, within RR42_RS32250 at 2623.952 kb on + strand, within RR42_RS32250 at 2623.952 kb on + strand, within RR42_RS32250 at 2623.952 kb on + strand, within RR42_RS32250 at 2623.952 kb on + strand, within RR42_RS32250 at 2623.953 kb on - strand, within RR42_RS32250 at 2623.953 kb on - strand, within RR42_RS32250 at 2623.953 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.094 kb on - strand, within RR42_RS32250 at 2624.114 kb on + strand, within RR42_RS32250 at 2624.115 kb on - strand, within RR42_RS32250 at 2624.132 kb on + strand, within RR42_RS32250 at 2624.132 kb on + strand, within RR42_RS32250 at 2624.133 kb on - strand, within RR42_RS32250 at 2625.121 kb on + strand at 2625.199 kb on + strand at 2625.200 kb on - strand at 2625.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,622,875 + RR42_RS32245 0.79 -1.5 2,622,876 - RR42_RS32245 0.79 -3.4 2,622,876 - RR42_RS32245 0.79 -3.3 2,622,876 - RR42_RS32245 0.79 -2.1 2,623,173 + -0.5 2,623,173 + -3.5 2,623,173 + -3.6 2,623,173 + -2.1 2,623,174 - -1.4 2,623,174 - -0.7 2,623,174 - -2.2 2,623,174 - -2.5 2,623,174 - -3.8 2,623,174 - -2.3 2,623,174 - -1.7 2,623,174 - -0.3 2,623,176 + -2.3 2,623,176 + -2.8 2,623,176 + +0.3 2,623,177 - -2.8 2,623,177 - -1.7 2,623,177 - -3.0 2,623,177 - -1.7 2,623,177 - -2.0 2,623,177 - -1.2 2,623,237 + -4.0 2,623,238 - +0.9 2,623,361 - -2.1 2,623,444 + +1.5 2,623,444 + +0.3 2,623,445 - -2.2 2,623,445 - -0.5 2,623,445 - -1.8 2,623,445 - -3.2 2,623,616 + RR42_RS32250 0.15 -2.0 2,623,616 + RR42_RS32250 0.15 -3.8 2,623,617 - RR42_RS32250 0.15 -3.1 2,623,617 - RR42_RS32250 0.15 -5.3 2,623,692 - RR42_RS32250 0.25 -2.3 2,623,718 - RR42_RS32250 0.28 -2.8 2,623,718 - RR42_RS32250 0.28 -3.3 2,623,898 + RR42_RS32250 0.50 -3.8 2,623,952 + RR42_RS32250 0.57 -3.7 2,623,952 + RR42_RS32250 0.57 -2.1 2,623,952 + RR42_RS32250 0.57 +0.9 2,623,952 + RR42_RS32250 0.57 -4.1 2,623,952 + RR42_RS32250 0.57 -2.5 2,623,952 + RR42_RS32250 0.57 -4.1 2,623,953 - RR42_RS32250 0.57 -3.2 2,623,953 - RR42_RS32250 0.57 -5.0 2,623,953 - RR42_RS32250 0.57 -0.7 2,624,090 - RR42_RS32250 0.74 -2.3 2,624,090 - RR42_RS32250 0.74 -2.2 2,624,090 - RR42_RS32250 0.74 -4.0 2,624,090 - RR42_RS32250 0.74 -3.2 2,624,090 - RR42_RS32250 0.74 -4.4 2,624,093 + RR42_RS32250 0.75 -2.6 2,624,093 + RR42_RS32250 0.75 -3.8 2,624,093 + RR42_RS32250 0.75 -3.8 2,624,093 + RR42_RS32250 0.75 -4.4 2,624,093 + RR42_RS32250 0.75 -4.5 2,624,093 + RR42_RS32250 0.75 -4.1 2,624,093 + RR42_RS32250 0.75 -3.8 2,624,094 - RR42_RS32250 0.75 -4.9 2,624,114 + RR42_RS32250 0.77 -3.1 2,624,115 - RR42_RS32250 0.77 -4.7 2,624,132 + RR42_RS32250 0.79 -3.3 2,624,132 + RR42_RS32250 0.79 -3.5 2,624,133 - RR42_RS32250 0.80 -2.1 2,625,121 + +0.9 2,625,199 + -0.7 2,625,200 - -0.8 2,625,200 - -1.4
Or see this region's nucleotide sequence