Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32060 and RR42_RS32065 are separated by 3 nucleotides RR42_RS32065 and RR42_RS32070 are separated by 95 nucleotides RR42_RS32070 and RR42_RS32075 are separated by 40 nucleotides
RR42_RS32060: RR42_RS32060 - protocatechuate 3,4-dioxygenase, at 2,570,217 to 2,570,795
_RS32060
RR42_RS32065: RR42_RS32065 - protocatechuate 3,4-dioxygenase, beta subunit (EC 1.13.11.3) (from data) , at 2,570,799 to 2,571,524
_RS32065
RR42_RS32070: RR42_RS32070 - LysR family transcriptional regulator, at 2,571,620 to 2,572,570
_RS32070
RR42_RS32075: RR42_RS32075 - EmrB/QacA subfamily drug resistance transporter, at 2,572,611 to 2,574,062
_RS32075
Position (kb)
2571
2572
2573 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2570.759 kb on - strand at 2570.789 kb on - strand at 2570.800 kb on + strand at 2570.800 kb on + strand at 2570.801 kb on - strand at 2570.837 kb on + strand at 2570.974 kb on + strand, within RR42_RS32065 at 2570.974 kb on + strand, within RR42_RS32065 at 2570.974 kb on + strand, within RR42_RS32065 at 2570.974 kb on + strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2571.001 kb on + strand, within RR42_RS32065 at 2571.002 kb on - strand, within RR42_RS32065 at 2571.029 kb on + strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.187 kb on + strand, within RR42_RS32065 at 2571.187 kb on + strand, within RR42_RS32065 at 2571.449 kb on - strand, within RR42_RS32065 at 2571.469 kb on + strand at 2571.527 kb on + strand at 2571.528 kb on - strand at 2571.528 kb on - strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.582 kb on + strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.583 kb on - strand at 2571.739 kb on - strand, within RR42_RS32070 at 2571.972 kb on + strand, within RR42_RS32070 at 2571.972 kb on + strand, within RR42_RS32070 at 2572.483 kb on - strand at 2572.483 kb on - strand at 2572.506 kb on + strand at 2572.568 kb on + strand at 2572.591 kb on - strand at 2572.601 kb on + strand at 2572.601 kb on + strand at 2572.613 kb on - strand at 2572.700 kb on + strand at 2572.700 kb on + strand at 2572.700 kb on + strand at 2572.701 kb on - strand at 2572.701 kb on - strand at 2572.718 kb on + strand at 2572.719 kb on - strand at 2572.719 kb on - strand at 2572.959 kb on + strand, within RR42_RS32075 at 2573.027 kb on + strand, within RR42_RS32075 at 2573.027 kb on + strand, within RR42_RS32075 at 2573.027 kb on + strand, within RR42_RS32075 at 2573.243 kb on + strand, within RR42_RS32075 at 2573.244 kb on - strand, within RR42_RS32075 at 2573.244 kb on - strand, within RR42_RS32075 at 2573.305 kb on + strand, within RR42_RS32075 at 2573.305 kb on + strand, within RR42_RS32075 at 2573.306 kb on - strand, within RR42_RS32075 at 2573.306 kb on - strand, within RR42_RS32075 at 2573.479 kb on + strand, within RR42_RS32075 at 2573.479 kb on + strand, within RR42_RS32075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,570,759 - -0.4 2,570,789 - +0.3 2,570,800 + -0.0 2,570,800 + +0.1 2,570,801 - -0.8 2,570,837 + -1.2 2,570,974 + RR42_RS32065 0.24 -0.8 2,570,974 + RR42_RS32065 0.24 -0.4 2,570,974 + RR42_RS32065 0.24 +1.2 2,570,974 + RR42_RS32065 0.24 -0.0 2,570,975 - RR42_RS32065 0.24 +0.4 2,570,975 - RR42_RS32065 0.24 -0.4 2,570,975 - RR42_RS32065 0.24 +1.8 2,570,975 - RR42_RS32065 0.24 -0.7 2,571,001 + RR42_RS32065 0.28 -0.2 2,571,002 - RR42_RS32065 0.28 -0.2 2,571,029 + RR42_RS32065 0.32 +0.6 2,571,030 - RR42_RS32065 0.32 -0.6 2,571,030 - RR42_RS32065 0.32 -1.5 2,571,030 - RR42_RS32065 0.32 -0.4 2,571,030 - RR42_RS32065 0.32 +0.6 2,571,030 - RR42_RS32065 0.32 -2.9 2,571,187 + RR42_RS32065 0.53 -0.4 2,571,187 + RR42_RS32065 0.53 -0.4 2,571,449 - RR42_RS32065 0.90 -2.5 2,571,469 + +0.3 2,571,527 + +1.0 2,571,528 - +0.6 2,571,528 - -1.8 2,571,582 + -0.1 2,571,582 + +0.3 2,571,582 + -1.2 2,571,582 + -0.5 2,571,582 + +1.8 2,571,582 + -0.1 2,571,582 + -1.1 2,571,582 + +0.0 2,571,582 + -1.3 2,571,582 + +0.8 2,571,582 + -0.8 2,571,583 - +2.0 2,571,583 - -1.0 2,571,583 - +0.2 2,571,583 - +0.3 2,571,583 - -0.8 2,571,583 - +0.9 2,571,583 - -0.4 2,571,583 - -0.0 2,571,583 - -0.2 2,571,583 - +0.8 2,571,583 - -0.3 2,571,583 - -2.0 2,571,583 - +0.0 2,571,583 - -1.2 2,571,583 - -1.2 2,571,583 - -0.3 2,571,583 - -0.7 2,571,583 - +0.3 2,571,583 - -0.6 2,571,583 - -0.2 2,571,583 - +1.3 2,571,739 - RR42_RS32070 0.13 -0.2 2,571,972 + RR42_RS32070 0.37 -2.8 2,571,972 + RR42_RS32070 0.37 -0.1 2,572,483 - +0.7 2,572,483 - +0.4 2,572,506 + +0.2 2,572,568 + +0.3 2,572,591 - +0.7 2,572,601 + -0.5 2,572,601 + +0.4 2,572,613 - +0.3 2,572,700 + -0.2 2,572,700 + -1.5 2,572,700 + -0.0 2,572,701 - +2.0 2,572,701 - -0.2 2,572,718 + +0.4 2,572,719 - +0.8 2,572,719 - -0.0 2,572,959 + RR42_RS32075 0.24 -0.1 2,573,027 + RR42_RS32075 0.29 -0.8 2,573,027 + RR42_RS32075 0.29 -1.2 2,573,027 + RR42_RS32075 0.29 +0.6 2,573,243 + RR42_RS32075 0.44 +1.4 2,573,244 - RR42_RS32075 0.44 -1.1 2,573,244 - RR42_RS32075 0.44 +0.7 2,573,305 + RR42_RS32075 0.48 -2.2 2,573,305 + RR42_RS32075 0.48 -0.1 2,573,306 - RR42_RS32075 0.48 -2.8 2,573,306 - RR42_RS32075 0.48 -0.3 2,573,479 + RR42_RS32075 0.60 -1.8 2,573,479 + RR42_RS32075 0.60 -0.1
Or see this region's nucleotide sequence