Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31890 and RR42_RS31895 are separated by 19 nucleotides RR42_RS31895 and RR42_RS31900 are separated by 305 nucleotides RR42_RS31900 and RR42_RS31905 are separated by 26 nucleotides
RR42_RS31890: RR42_RS31890 - acetyl-CoA acetyltransferase, at 2,532,238 to 2,533,440
_RS31890
RR42_RS31895: RR42_RS31895 - dehydratase, at 2,533,460 to 2,533,915
_RS31895
RR42_RS31900: RR42_RS31900 - short-chain dehydrogenase, at 2,534,221 to 2,535,018
_RS31900
RR42_RS31905: RR42_RS31905 - acyl-CoA dehydrogenase, at 2,535,045 to 2,536,271
_RS31905
Position (kb)
2534
2535
2536 Strain fitness (log2 ratio)
-2
-1
0
1 at 2533.904 kb on + strand at 2533.905 kb on - strand at 2534.097 kb on - strand at 2534.097 kb on - strand at 2534.264 kb on + strand at 2534.264 kb on + strand at 2534.265 kb on - strand at 2534.501 kb on + strand, within RR42_RS31900 at 2534.502 kb on - strand, within RR42_RS31900 at 2534.580 kb on + strand, within RR42_RS31900 at 2534.581 kb on - strand, within RR42_RS31900 at 2534.669 kb on - strand, within RR42_RS31900 at 2534.987 kb on + strand at 2534.988 kb on - strand at 2534.988 kb on - strand at 2535.098 kb on + strand at 2535.098 kb on + strand at 2535.099 kb on - strand at 2535.127 kb on + strand at 2535.265 kb on + strand, within RR42_RS31905 at 2535.268 kb on + strand, within RR42_RS31905 at 2535.268 kb on + strand, within RR42_RS31905 at 2535.269 kb on - strand, within RR42_RS31905 at 2535.274 kb on + strand, within RR42_RS31905 at 2535.275 kb on - strand, within RR42_RS31905 at 2535.367 kb on + strand, within RR42_RS31905 at 2535.368 kb on - strand, within RR42_RS31905 at 2535.981 kb on - strand, within RR42_RS31905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,533,904 + +0.2 2,533,905 - -0.2 2,534,097 - -0.8 2,534,097 - -1.0 2,534,264 + +0.1 2,534,264 + -0.1 2,534,265 - +0.3 2,534,501 + RR42_RS31900 0.35 -1.7 2,534,502 - RR42_RS31900 0.35 -1.8 2,534,580 + RR42_RS31900 0.45 +0.8 2,534,581 - RR42_RS31900 0.45 -1.0 2,534,669 - RR42_RS31900 0.56 -0.6 2,534,987 + +1.1 2,534,988 - -1.8 2,534,988 - +0.4 2,535,098 + +0.8 2,535,098 + +0.2 2,535,099 - -0.2 2,535,127 + -0.8 2,535,265 + RR42_RS31905 0.18 +0.5 2,535,268 + RR42_RS31905 0.18 -0.1 2,535,268 + RR42_RS31905 0.18 -0.8 2,535,269 - RR42_RS31905 0.18 +0.2 2,535,274 + RR42_RS31905 0.19 -0.9 2,535,275 - RR42_RS31905 0.19 -0.0 2,535,367 + RR42_RS31905 0.26 -1.0 2,535,368 - RR42_RS31905 0.26 -0.3 2,535,981 - RR42_RS31905 0.76 -2.0
Or see this region's nucleotide sequence