Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31190 and RR42_RS31195 overlap by 4 nucleotides RR42_RS31195 and RR42_RS31200 are separated by 2 nucleotides RR42_RS31200 and RR42_RS31205 overlap by 4 nucleotides RR42_RS31205 and RR42_RS31210 are separated by 38 nucleotides
RR42_RS31190: RR42_RS31190 - 3-ketoacyl-ACP reductase, at 2,375,509 to 2,376,240
_RS31190
RR42_RS31195: RR42_RS31195 - dehydratase, at 2,376,237 to 2,376,707
_RS31195
RR42_RS31200: RR42_RS31200 - 3-oxoacyl-ACP synthase, at 2,376,710 to 2,377,891
_RS31200
RR42_RS31205: RR42_RS31205 - phospho-N-acetylmuramoyl-pentapeptide transferase, at 2,377,888 to 2,378,496
_RS31205
RR42_RS31210: RR42_RS31210 - FAD-dependent oxidoreductase, at 2,378,535 to 2,379,764
_RS31210
Position (kb)
2376
2377
2378 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.891 kb on + strand, within RR42_RS31190 at 2375.892 kb on - strand, within RR42_RS31190 at 2375.994 kb on - strand, within RR42_RS31190 at 2375.997 kb on - strand, within RR42_RS31190 at 2376.159 kb on - strand, within RR42_RS31190 at 2376.384 kb on + strand, within RR42_RS31195 at 2376.384 kb on + strand, within RR42_RS31195 at 2376.385 kb on - strand, within RR42_RS31195 at 2376.385 kb on - strand, within RR42_RS31195 at 2376.431 kb on - strand, within RR42_RS31195 at 2376.523 kb on + strand, within RR42_RS31195 at 2376.523 kb on + strand, within RR42_RS31195 at 2376.524 kb on - strand, within RR42_RS31195 at 2376.524 kb on - strand, within RR42_RS31195 at 2376.725 kb on + strand at 2376.726 kb on - strand at 2376.726 kb on - strand at 2377.056 kb on + strand, within RR42_RS31200 at 2377.057 kb on - strand, within RR42_RS31200 at 2377.437 kb on + strand, within RR42_RS31200 at 2377.438 kb on - strand, within RR42_RS31200 at 2377.582 kb on + strand, within RR42_RS31200 at 2377.671 kb on + strand, within RR42_RS31200 at 2377.773 kb on + strand at 2377.774 kb on - strand at 2377.879 kb on + strand at 2377.941 kb on - strand at 2378.121 kb on + strand, within RR42_RS31205 at 2378.122 kb on - strand, within RR42_RS31205 at 2378.300 kb on + strand, within RR42_RS31205 at 2378.300 kb on + strand, within RR42_RS31205 at 2378.301 kb on - strand, within RR42_RS31205 at 2378.301 kb on - strand, within RR42_RS31205 at 2378.614 kb on + strand at 2378.614 kb on + strand at 2378.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,375,780 + RR42_RS31190 0.37 -2.0 2,375,780 + RR42_RS31190 0.37 +1.0 2,375,780 + RR42_RS31190 0.37 +0.6 2,375,780 + RR42_RS31190 0.37 +0.5 2,375,780 + RR42_RS31190 0.37 +1.4 2,375,781 - RR42_RS31190 0.37 +1.8 2,375,781 - RR42_RS31190 0.37 +0.7 2,375,781 - RR42_RS31190 0.37 +1.7 2,375,781 - RR42_RS31190 0.37 -1.0 2,375,891 + RR42_RS31190 0.52 -0.3 2,375,892 - RR42_RS31190 0.52 +0.3 2,375,994 - RR42_RS31190 0.66 -1.1 2,375,997 - RR42_RS31190 0.67 -0.9 2,376,159 - RR42_RS31190 0.89 +0.4 2,376,384 + RR42_RS31195 0.31 -0.6 2,376,384 + RR42_RS31195 0.31 -0.2 2,376,385 - RR42_RS31195 0.31 +0.7 2,376,385 - RR42_RS31195 0.31 -0.0 2,376,431 - RR42_RS31195 0.41 -0.4 2,376,523 + RR42_RS31195 0.61 -0.8 2,376,523 + RR42_RS31195 0.61 +0.8 2,376,524 - RR42_RS31195 0.61 -0.9 2,376,524 - RR42_RS31195 0.61 -0.4 2,376,725 + -1.6 2,376,726 - -0.8 2,376,726 - +0.0 2,377,056 + RR42_RS31200 0.29 -1.1 2,377,057 - RR42_RS31200 0.29 +0.6 2,377,437 + RR42_RS31200 0.62 -0.2 2,377,438 - RR42_RS31200 0.62 +1.0 2,377,582 + RR42_RS31200 0.74 +0.0 2,377,671 + RR42_RS31200 0.81 +1.2 2,377,773 + +0.7 2,377,774 - +0.5 2,377,879 + +1.1 2,377,941 - -0.1 2,378,121 + RR42_RS31205 0.38 +0.3 2,378,122 - RR42_RS31205 0.38 -0.9 2,378,300 + RR42_RS31205 0.68 +1.0 2,378,300 + RR42_RS31205 0.68 +1.3 2,378,301 - RR42_RS31205 0.68 -3.2 2,378,301 - RR42_RS31205 0.68 -0.3 2,378,614 + +0.3 2,378,614 + +0.5 2,378,615 - -0.4
Or see this region's nucleotide sequence