Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31170 and RR42_RS31175 overlap by 84 nucleotides RR42_RS31175 and RR42_RS31180 are separated by 90 nucleotides RR42_RS31180 and RR42_RS31185 are separated by 91 nucleotides RR42_RS31185 and RR42_RS31190 overlap by 4 nucleotides RR42_RS31190 and RR42_RS31195 overlap by 4 nucleotides
RR42_RS31170: RR42_RS31170 - ACP synthase, at 2,373,223 to 2,373,453
_RS31170
RR42_RS31175: RR42_RS31175 - hypothetical protein, at 2,373,370 to 2,373,651
_RS31175
RR42_RS31180: RR42_RS31180 - excinuclease ATPase subunit, at 2,373,742 to 2,374,194
_RS31180
RR42_RS31185: RR42_RS31185 - 3-oxoacyl-ACP synthase, at 2,374,286 to 2,375,512
_RS31185
RR42_RS31190: RR42_RS31190 - 3-ketoacyl-ACP reductase, at 2,375,509 to 2,376,240
_RS31190
RR42_RS31195: RR42_RS31195 - dehydratase, at 2,376,237 to 2,376,707
_RS31195
Position (kb)
2374
2375
2376 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2373.682 kb on + strand at 2373.683 kb on - strand at 2373.743 kb on + strand at 2373.764 kb on + strand at 2373.764 kb on + strand at 2373.764 kb on + strand at 2373.765 kb on - strand at 2373.765 kb on - strand at 2373.765 kb on - strand at 2373.816 kb on - strand, within RR42_RS31180 at 2373.848 kb on + strand, within RR42_RS31180 at 2373.848 kb on + strand, within RR42_RS31180 at 2373.848 kb on + strand, within RR42_RS31180 at 2373.849 kb on - strand, within RR42_RS31180 at 2373.849 kb on - strand, within RR42_RS31180 at 2373.849 kb on - strand, within RR42_RS31180 at 2373.851 kb on + strand, within RR42_RS31180 at 2373.852 kb on - strand, within RR42_RS31180 at 2373.852 kb on - strand, within RR42_RS31180 at 2373.852 kb on - strand, within RR42_RS31180 at 2374.109 kb on + strand, within RR42_RS31180 at 2374.109 kb on + strand, within RR42_RS31180 at 2374.109 kb on + strand, within RR42_RS31180 at 2374.110 kb on - strand, within RR42_RS31180 at 2374.110 kb on - strand, within RR42_RS31180 at 2374.247 kb on - strand at 2374.274 kb on - strand at 2374.389 kb on + strand at 2374.390 kb on - strand at 2374.507 kb on + strand, within RR42_RS31185 at 2374.507 kb on + strand, within RR42_RS31185 at 2374.507 kb on + strand, within RR42_RS31185 at 2374.508 kb on - strand, within RR42_RS31185 at 2374.508 kb on - strand, within RR42_RS31185 at 2374.508 kb on - strand, within RR42_RS31185 at 2374.508 kb on - strand, within RR42_RS31185 at 2374.516 kb on + strand, within RR42_RS31185 at 2374.517 kb on - strand, within RR42_RS31185 at 2374.518 kb on + strand, within RR42_RS31185 at 2374.518 kb on + strand, within RR42_RS31185 at 2374.518 kb on + strand, within RR42_RS31185 at 2374.518 kb on + strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.623 kb on + strand, within RR42_RS31185 at 2374.623 kb on + strand, within RR42_RS31185 at 2374.989 kb on + strand, within RR42_RS31185 at 2374.989 kb on + strand, within RR42_RS31185 at 2375.035 kb on + strand, within RR42_RS31185 at 2375.035 kb on + strand, within RR42_RS31185 at 2375.053 kb on + strand, within RR42_RS31185 at 2375.109 kb on + strand, within RR42_RS31185 at 2375.109 kb on + strand, within RR42_RS31185 at 2375.110 kb on - strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.145 kb on - strand, within RR42_RS31185 at 2375.199 kb on + strand, within RR42_RS31185 at 2375.200 kb on - strand, within RR42_RS31185 at 2375.328 kb on + strand, within RR42_RS31185 at 2375.328 kb on + strand, within RR42_RS31185 at 2375.329 kb on - strand, within RR42_RS31185 at 2375.329 kb on - strand, within RR42_RS31185 at 2375.553 kb on + strand at 2375.553 kb on + strand at 2375.554 kb on - strand at 2375.555 kb on + strand at 2375.556 kb on - strand at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.891 kb on + strand, within RR42_RS31190 at 2375.892 kb on - strand, within RR42_RS31190 at 2375.994 kb on - strand, within RR42_RS31190 at 2375.997 kb on - strand, within RR42_RS31190 at 2376.159 kb on - strand, within RR42_RS31190 at 2376.384 kb on + strand, within RR42_RS31195 at 2376.384 kb on + strand, within RR42_RS31195 at 2376.385 kb on - strand, within RR42_RS31195 at 2376.385 kb on - strand, within RR42_RS31195 at 2376.431 kb on - strand, within RR42_RS31195
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,373,682 + -0.1 2,373,683 - -0.3 2,373,743 + +0.3 2,373,764 + -0.1 2,373,764 + +0.7 2,373,764 + +1.1 2,373,765 - +0.5 2,373,765 - +0.2 2,373,765 - +0.9 2,373,816 - RR42_RS31180 0.16 -1.9 2,373,848 + RR42_RS31180 0.23 +0.1 2,373,848 + RR42_RS31180 0.23 +0.9 2,373,848 + RR42_RS31180 0.23 -0.2 2,373,849 - RR42_RS31180 0.24 +1.0 2,373,849 - RR42_RS31180 0.24 +0.7 2,373,849 - RR42_RS31180 0.24 -1.7 2,373,851 + RR42_RS31180 0.24 +0.9 2,373,852 - RR42_RS31180 0.24 -1.1 2,373,852 - RR42_RS31180 0.24 -0.8 2,373,852 - RR42_RS31180 0.24 +1.1 2,374,109 + RR42_RS31180 0.81 +2.1 2,374,109 + RR42_RS31180 0.81 +0.4 2,374,109 + RR42_RS31180 0.81 +0.5 2,374,110 - RR42_RS31180 0.81 +1.0 2,374,110 - RR42_RS31180 0.81 +0.1 2,374,247 - -0.2 2,374,274 - +0.8 2,374,389 + +1.0 2,374,390 - +1.6 2,374,507 + RR42_RS31185 0.18 +2.2 2,374,507 + RR42_RS31185 0.18 +2.7 2,374,507 + RR42_RS31185 0.18 +1.5 2,374,508 - RR42_RS31185 0.18 +1.4 2,374,508 - RR42_RS31185 0.18 +1.7 2,374,508 - RR42_RS31185 0.18 +1.3 2,374,508 - RR42_RS31185 0.18 +1.8 2,374,516 + RR42_RS31185 0.19 +1.2 2,374,517 - RR42_RS31185 0.19 +1.3 2,374,518 + RR42_RS31185 0.19 +1.6 2,374,518 + RR42_RS31185 0.19 +2.1 2,374,518 + RR42_RS31185 0.19 +2.1 2,374,518 + RR42_RS31185 0.19 +2.6 2,374,519 - RR42_RS31185 0.19 +1.6 2,374,519 - RR42_RS31185 0.19 +3.4 2,374,519 - RR42_RS31185 0.19 +1.9 2,374,519 - RR42_RS31185 0.19 +0.1 2,374,519 - RR42_RS31185 0.19 +1.9 2,374,519 - RR42_RS31185 0.19 +1.2 2,374,519 - RR42_RS31185 0.19 +1.1 2,374,623 + RR42_RS31185 0.27 +2.4 2,374,623 + RR42_RS31185 0.27 +2.2 2,374,989 + RR42_RS31185 0.57 +1.5 2,374,989 + RR42_RS31185 0.57 +2.5 2,375,035 + RR42_RS31185 0.61 +2.0 2,375,035 + RR42_RS31185 0.61 +2.1 2,375,053 + RR42_RS31185 0.63 +1.8 2,375,109 + RR42_RS31185 0.67 +2.1 2,375,109 + RR42_RS31185 0.67 +0.9 2,375,110 - RR42_RS31185 0.67 +2.8 2,375,144 + RR42_RS31185 0.70 +2.4 2,375,144 + RR42_RS31185 0.70 +1.9 2,375,144 + RR42_RS31185 0.70 +2.5 2,375,144 + RR42_RS31185 0.70 +1.9 2,375,144 + RR42_RS31185 0.70 +2.4 2,375,145 - RR42_RS31185 0.70 +0.7 2,375,199 + RR42_RS31185 0.74 +2.3 2,375,200 - RR42_RS31185 0.74 +2.5 2,375,328 + RR42_RS31185 0.85 +1.4 2,375,328 + RR42_RS31185 0.85 +2.0 2,375,329 - RR42_RS31185 0.85 +1.6 2,375,329 - RR42_RS31185 0.85 +2.3 2,375,553 + +0.4 2,375,553 + +1.7 2,375,554 - -1.0 2,375,555 + -0.3 2,375,556 - -0.1 2,375,780 + RR42_RS31190 0.37 -2.0 2,375,780 + RR42_RS31190 0.37 +1.0 2,375,780 + RR42_RS31190 0.37 +0.6 2,375,780 + RR42_RS31190 0.37 +0.5 2,375,780 + RR42_RS31190 0.37 +1.4 2,375,781 - RR42_RS31190 0.37 +1.8 2,375,781 - RR42_RS31190 0.37 +0.7 2,375,781 - RR42_RS31190 0.37 +1.7 2,375,781 - RR42_RS31190 0.37 -1.0 2,375,891 + RR42_RS31190 0.52 -0.3 2,375,892 - RR42_RS31190 0.52 +0.3 2,375,994 - RR42_RS31190 0.66 -1.1 2,375,997 - RR42_RS31190 0.67 -0.9 2,376,159 - RR42_RS31190 0.89 +0.4 2,376,384 + RR42_RS31195 0.31 -0.6 2,376,384 + RR42_RS31195 0.31 -0.2 2,376,385 - RR42_RS31195 0.31 +0.7 2,376,385 - RR42_RS31195 0.31 -0.0 2,376,431 - RR42_RS31195 0.41 -0.4
Or see this region's nucleotide sequence