Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31050 and RR42_RS31055 are separated by 203 nucleotides RR42_RS31055 and RR42_RS31060 are separated by 90 nucleotides
RR42_RS31050: RR42_RS31050 - 3-hydroxyacyl-CoA dehydrogenase, at 2,345,985 to 2,346,956
_RS31050
RR42_RS31055: RR42_RS31055 - 3-hydroxybutyryl-CoA dehydrogenase, at 2,347,160 to 2,348,086
_RS31055
RR42_RS31060: RR42_RS31060 - diguanylate cyclase, at 2,348,177 to 2,349,748
_RS31060
Position (kb)
2347
2348
2349 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2346.185 kb on - strand, within RR42_RS31050 at 2346.185 kb on - strand, within RR42_RS31050 at 2346.185 kb on - strand, within RR42_RS31050 at 2346.185 kb on - strand, within RR42_RS31050 at 2346.192 kb on - strand, within RR42_RS31050 at 2346.587 kb on + strand, within RR42_RS31050 at 2346.588 kb on - strand, within RR42_RS31050 at 2346.588 kb on - strand, within RR42_RS31050 at 2346.818 kb on + strand, within RR42_RS31050 at 2346.819 kb on - strand, within RR42_RS31050 at 2346.897 kb on + strand at 2346.898 kb on - strand at 2347.081 kb on + strand at 2347.081 kb on + strand at 2347.081 kb on + strand at 2347.082 kb on - strand at 2347.640 kb on + strand, within RR42_RS31055 at 2347.931 kb on - strand, within RR42_RS31055 at 2348.148 kb on + strand at 2348.253 kb on + strand at 2348.253 kb on + strand at 2348.253 kb on + strand at 2348.254 kb on - strand at 2348.254 kb on - strand at 2348.500 kb on + strand, within RR42_RS31060 at 2348.500 kb on + strand, within RR42_RS31060 at 2348.501 kb on - strand, within RR42_RS31060 at 2348.501 kb on - strand, within RR42_RS31060 at 2348.613 kb on + strand, within RR42_RS31060 at 2348.614 kb on - strand, within RR42_RS31060 at 2348.614 kb on - strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.761 kb on - strand, within RR42_RS31060 at 2348.761 kb on - strand, within RR42_RS31060 at 2348.872 kb on + strand, within RR42_RS31060 at 2348.872 kb on + strand, within RR42_RS31060 at 2348.872 kb on + strand, within RR42_RS31060 at 2348.873 kb on - strand, within RR42_RS31060 at 2348.873 kb on - strand, within RR42_RS31060 at 2349.006 kb on + strand at 2349.041 kb on + strand, within RR42_RS31060 at 2349.041 kb on + strand, within RR42_RS31060 at 2349.071 kb on + strand, within RR42_RS31060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,346,185 - RR42_RS31050 0.21 -1.5 2,346,185 - RR42_RS31050 0.21 -0.2 2,346,185 - RR42_RS31050 0.21 -0.4 2,346,185 - RR42_RS31050 0.21 +1.4 2,346,192 - RR42_RS31050 0.21 +0.3 2,346,587 + RR42_RS31050 0.62 -0.1 2,346,588 - RR42_RS31050 0.62 +1.8 2,346,588 - RR42_RS31050 0.62 +0.2 2,346,818 + RR42_RS31050 0.86 -0.8 2,346,819 - RR42_RS31050 0.86 +0.5 2,346,897 + +1.1 2,346,898 - -0.5 2,347,081 + -0.2 2,347,081 + -0.2 2,347,081 + -1.0 2,347,082 - +0.7 2,347,640 + RR42_RS31055 0.52 -2.2 2,347,931 - RR42_RS31055 0.83 +0.1 2,348,148 + +0.1 2,348,253 + +0.9 2,348,253 + -0.1 2,348,253 + +0.7 2,348,254 - -2.2 2,348,254 - +0.1 2,348,500 + RR42_RS31060 0.21 +0.1 2,348,500 + RR42_RS31060 0.21 -0.3 2,348,501 - RR42_RS31060 0.21 -1.6 2,348,501 - RR42_RS31060 0.21 +0.5 2,348,613 + RR42_RS31060 0.28 +0.5 2,348,614 - RR42_RS31060 0.28 +0.2 2,348,614 - RR42_RS31060 0.28 -1.0 2,348,760 + RR42_RS31060 0.37 -0.2 2,348,760 + RR42_RS31060 0.37 -0.4 2,348,760 + RR42_RS31060 0.37 -0.3 2,348,760 + RR42_RS31060 0.37 +0.3 2,348,761 - RR42_RS31060 0.37 -0.6 2,348,761 - RR42_RS31060 0.37 -0.1 2,348,872 + RR42_RS31060 0.44 +0.7 2,348,872 + RR42_RS31060 0.44 -0.4 2,348,872 + RR42_RS31060 0.44 -0.5 2,348,873 - RR42_RS31060 0.44 -0.4 2,348,873 - RR42_RS31060 0.44 -0.2 2,349,006 + +0.7 2,349,041 + RR42_RS31060 0.55 +0.8 2,349,041 + RR42_RS31060 0.55 +1.2 2,349,071 + RR42_RS31060 0.57 +0.3
Or see this region's nucleotide sequence