Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30655 and RR42_RS30660 are separated by 145 nucleotides RR42_RS30660 and RR42_RS30665 are separated by 280 nucleotides
RR42_RS30655: RR42_RS30655 - LysR family transcriptional regulator, at 2,252,026 to 2,252,949
_RS30655
RR42_RS30660: RR42_RS30660 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase, at 2,253,095 to 2,255,482
_RS30660
RR42_RS30665: RR42_RS30665 - TonB-dependent receptor, at 2,255,763 to 2,257,874
_RS30665
Position (kb)
2253
2254
2255
2256 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2252.108 kb on + strand at 2252.108 kb on + strand at 2252.109 kb on - strand at 2252.109 kb on - strand at 2252.145 kb on - strand, within RR42_RS30655 at 2252.192 kb on + strand, within RR42_RS30655 at 2252.193 kb on - strand, within RR42_RS30655 at 2252.207 kb on + strand, within RR42_RS30655 at 2252.207 kb on + strand, within RR42_RS30655 at 2252.208 kb on - strand, within RR42_RS30655 at 2252.208 kb on - strand, within RR42_RS30655 at 2252.646 kb on + strand, within RR42_RS30655 at 2252.647 kb on - strand, within RR42_RS30655 at 2252.974 kb on + strand at 2252.975 kb on - strand at 2253.082 kb on + strand at 2253.110 kb on - strand at 2253.290 kb on + strand at 2253.291 kb on - strand at 2253.291 kb on - strand at 2253.291 kb on - strand at 2253.377 kb on + strand, within RR42_RS30660 at 2253.446 kb on + strand, within RR42_RS30660 at 2253.578 kb on + strand, within RR42_RS30660 at 2253.578 kb on + strand, within RR42_RS30660 at 2253.650 kb on + strand, within RR42_RS30660 at 2253.650 kb on + strand, within RR42_RS30660 at 2253.651 kb on - strand, within RR42_RS30660 at 2253.752 kb on + strand, within RR42_RS30660 at 2253.764 kb on + strand, within RR42_RS30660 at 2253.805 kb on + strand, within RR42_RS30660 at 2253.809 kb on + strand, within RR42_RS30660 at 2253.809 kb on + strand, within RR42_RS30660 at 2254.574 kb on + strand, within RR42_RS30660 at 2254.599 kb on - strand, within RR42_RS30660 at 2254.631 kb on + strand, within RR42_RS30660 at 2254.631 kb on + strand, within RR42_RS30660 at 2254.676 kb on + strand, within RR42_RS30660 at 2254.713 kb on - strand, within RR42_RS30660 at 2255.027 kb on + strand, within RR42_RS30660 at 2255.168 kb on + strand, within RR42_RS30660 at 2255.467 kb on + strand at 2255.483 kb on + strand at 2255.522 kb on + strand at 2255.523 kb on - strand at 2255.523 kb on - strand at 2255.535 kb on - strand at 2255.697 kb on + strand at 2255.720 kb on - strand at 2255.720 kb on - strand at 2256.007 kb on - strand, within RR42_RS30665 at 2256.018 kb on + strand, within RR42_RS30665 at 2256.019 kb on - strand, within RR42_RS30665 at 2256.019 kb on - strand, within RR42_RS30665 at 2256.019 kb on - strand, within RR42_RS30665 at 2256.063 kb on + strand, within RR42_RS30665 at 2256.063 kb on + strand, within RR42_RS30665 at 2256.063 kb on + strand, within RR42_RS30665 at 2256.063 kb on + strand, within RR42_RS30665 at 2256.063 kb on + strand, within RR42_RS30665 at 2256.064 kb on - strand, within RR42_RS30665 at 2256.064 kb on - strand, within RR42_RS30665 at 2256.079 kb on - strand, within RR42_RS30665 at 2256.130 kb on - strand, within RR42_RS30665 at 2256.130 kb on - strand, within RR42_RS30665 at 2256.198 kb on + strand, within RR42_RS30665 at 2256.201 kb on + strand, within RR42_RS30665 at 2256.202 kb on - strand, within RR42_RS30665 at 2256.474 kb on + strand, within RR42_RS30665
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,252,108 + -0.4 2,252,108 + +0.4 2,252,109 - -0.4 2,252,109 - +0.3 2,252,145 - RR42_RS30655 0.13 +0.3 2,252,192 + RR42_RS30655 0.18 +0.1 2,252,193 - RR42_RS30655 0.18 -0.5 2,252,207 + RR42_RS30655 0.20 -1.4 2,252,207 + RR42_RS30655 0.20 +0.5 2,252,208 - RR42_RS30655 0.20 +0.2 2,252,208 - RR42_RS30655 0.20 -0.3 2,252,646 + RR42_RS30655 0.67 -1.9 2,252,647 - RR42_RS30655 0.67 -0.7 2,252,974 + -0.7 2,252,975 - -1.0 2,253,082 + +2.1 2,253,110 - +1.4 2,253,290 + -1.2 2,253,291 - -1.9 2,253,291 - +1.8 2,253,291 - -1.2 2,253,377 + RR42_RS30660 0.12 -0.6 2,253,446 + RR42_RS30660 0.15 -0.3 2,253,578 + RR42_RS30660 0.20 -0.5 2,253,578 + RR42_RS30660 0.20 -1.5 2,253,650 + RR42_RS30660 0.23 -0.7 2,253,650 + RR42_RS30660 0.23 -1.1 2,253,651 - RR42_RS30660 0.23 -0.9 2,253,752 + RR42_RS30660 0.28 -0.3 2,253,764 + RR42_RS30660 0.28 +1.0 2,253,805 + RR42_RS30660 0.30 -0.1 2,253,809 + RR42_RS30660 0.30 +0.0 2,253,809 + RR42_RS30660 0.30 -0.2 2,254,574 + RR42_RS30660 0.62 +1.5 2,254,599 - RR42_RS30660 0.63 +0.1 2,254,631 + RR42_RS30660 0.64 +1.3 2,254,631 + RR42_RS30660 0.64 -3.3 2,254,676 + RR42_RS30660 0.66 -0.9 2,254,713 - RR42_RS30660 0.68 -1.7 2,255,027 + RR42_RS30660 0.81 -0.6 2,255,168 + RR42_RS30660 0.87 -0.0 2,255,467 + -0.1 2,255,483 + -2.2 2,255,522 + +0.8 2,255,523 - +1.0 2,255,523 - +0.2 2,255,535 - -1.3 2,255,697 + +0.3 2,255,720 - +0.3 2,255,720 - +1.1 2,256,007 - RR42_RS30665 0.12 -0.6 2,256,018 + RR42_RS30665 0.12 +1.6 2,256,019 - RR42_RS30665 0.12 +0.8 2,256,019 - RR42_RS30665 0.12 +0.2 2,256,019 - RR42_RS30665 0.12 +1.1 2,256,063 + RR42_RS30665 0.14 +1.5 2,256,063 + RR42_RS30665 0.14 -0.2 2,256,063 + RR42_RS30665 0.14 -0.7 2,256,063 + RR42_RS30665 0.14 -0.3 2,256,063 + RR42_RS30665 0.14 +0.1 2,256,064 - RR42_RS30665 0.14 +0.7 2,256,064 - RR42_RS30665 0.14 -0.1 2,256,079 - RR42_RS30665 0.15 -0.2 2,256,130 - RR42_RS30665 0.17 +0.9 2,256,130 - RR42_RS30665 0.17 +1.1 2,256,198 + RR42_RS30665 0.21 +1.1 2,256,201 + RR42_RS30665 0.21 -1.7 2,256,202 - RR42_RS30665 0.21 +1.0 2,256,474 + RR42_RS30665 0.34 +0.1
Or see this region's nucleotide sequence