Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30610 and RR42_RS30615 are separated by 57 nucleotides RR42_RS30615 and RR42_RS30620 overlap by 4 nucleotides RR42_RS30620 and RR42_RS30625 are separated by 133 nucleotides
RR42_RS30610: RR42_RS30610 - cystathionine beta-lyase, at 2,245,319 to 2,246,536
_RS30610
RR42_RS30615: RR42_RS30615 - plasmid stabilization protein, at 2,246,594 to 2,246,839
_RS30615
RR42_RS30620: RR42_RS30620 - plasmid stabilization protein, at 2,246,836 to 2,247,255
_RS30620
RR42_RS30625: RR42_RS30625 - cob(II)yrinic acid a,c-diamide reductase, at 2,247,389 to 2,248,054
_RS30625
Position (kb)
2246
2247 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2245.610 kb on + strand, within RR42_RS30610 at 2245.652 kb on + strand, within RR42_RS30610 at 2245.653 kb on - strand, within RR42_RS30610 at 2245.715 kb on + strand, within RR42_RS30610 at 2245.715 kb on + strand, within RR42_RS30610 at 2245.716 kb on - strand, within RR42_RS30610 at 2245.772 kb on + strand, within RR42_RS30610 at 2245.772 kb on + strand, within RR42_RS30610 at 2245.773 kb on - strand, within RR42_RS30610 at 2245.773 kb on - strand, within RR42_RS30610 at 2245.799 kb on + strand, within RR42_RS30610 at 2245.892 kb on + strand, within RR42_RS30610 at 2245.892 kb on + strand, within RR42_RS30610 at 2245.892 kb on + strand, within RR42_RS30610 at 2245.893 kb on - strand, within RR42_RS30610 at 2245.893 kb on - strand, within RR42_RS30610 at 2245.918 kb on + strand, within RR42_RS30610 at 2245.918 kb on + strand, within RR42_RS30610 at 2245.918 kb on + strand, within RR42_RS30610 at 2245.919 kb on - strand, within RR42_RS30610 at 2245.943 kb on + strand, within RR42_RS30610 at 2245.944 kb on + strand, within RR42_RS30610 at 2245.944 kb on - strand, within RR42_RS30610 at 2245.944 kb on - strand, within RR42_RS30610 at 2245.944 kb on - strand, within RR42_RS30610 at 2245.944 kb on - strand, within RR42_RS30610 at 2245.984 kb on + strand, within RR42_RS30610 at 2246.060 kb on + strand, within RR42_RS30610 at 2246.061 kb on - strand, within RR42_RS30610 at 2246.061 kb on - strand, within RR42_RS30610 at 2246.061 kb on - strand, within RR42_RS30610 at 2246.290 kb on + strand, within RR42_RS30610 at 2246.554 kb on + strand at 2246.555 kb on - strand at 2246.555 kb on - strand at 2246.582 kb on + strand at 2246.583 kb on - strand at 2246.843 kb on + strand at 2246.843 kb on + strand at 2246.843 kb on + strand at 2246.850 kb on + strand at 2246.851 kb on - strand at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.901 kb on - strand, within RR42_RS30620 at 2246.932 kb on + strand, within RR42_RS30620 at 2246.933 kb on - strand, within RR42_RS30620 at 2246.966 kb on + strand, within RR42_RS30620 at 2246.967 kb on - strand, within RR42_RS30620 at 2246.967 kb on - strand, within RR42_RS30620 at 2246.967 kb on - strand, within RR42_RS30620 at 2246.967 kb on - strand, within RR42_RS30620 at 2246.968 kb on + strand, within RR42_RS30620 at 2246.968 kb on + strand, within RR42_RS30620 at 2247.059 kb on + strand, within RR42_RS30620 at 2247.059 kb on + strand, within RR42_RS30620 at 2247.059 kb on + strand, within RR42_RS30620 at 2247.060 kb on - strand, within RR42_RS30620 at 2247.060 kb on - strand, within RR42_RS30620 at 2247.060 kb on - strand, within RR42_RS30620 at 2247.079 kb on - strand, within RR42_RS30620 at 2247.079 kb on - strand, within RR42_RS30620 at 2247.091 kb on + strand, within RR42_RS30620 at 2247.092 kb on - strand, within RR42_RS30620 at 2247.162 kb on + strand, within RR42_RS30620 at 2247.162 kb on + strand, within RR42_RS30620 at 2247.162 kb on + strand, within RR42_RS30620 at 2247.162 kb on + strand, within RR42_RS30620 at 2247.163 kb on - strand, within RR42_RS30620 at 2247.163 kb on - strand, within RR42_RS30620 at 2247.163 kb on - strand, within RR42_RS30620 at 2247.222 kb on - strand at 2247.222 kb on - strand at 2247.287 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.440 kb on + strand at 2247.440 kb on + strand at 2247.441 kb on - strand at 2247.441 kb on - strand at 2247.441 kb on - strand at 2247.574 kb on + strand, within RR42_RS30625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,245,610 + RR42_RS30610 0.24 -0.6 2,245,652 + RR42_RS30610 0.27 +0.7 2,245,653 - RR42_RS30610 0.27 +0.2 2,245,715 + RR42_RS30610 0.33 -0.5 2,245,715 + RR42_RS30610 0.33 -0.9 2,245,716 - RR42_RS30610 0.33 -0.1 2,245,772 + RR42_RS30610 0.37 +1.2 2,245,772 + RR42_RS30610 0.37 -2.9 2,245,773 - RR42_RS30610 0.37 -0.5 2,245,773 - RR42_RS30610 0.37 -0.5 2,245,799 + RR42_RS30610 0.39 -0.9 2,245,892 + RR42_RS30610 0.47 -0.5 2,245,892 + RR42_RS30610 0.47 -1.2 2,245,892 + RR42_RS30610 0.47 +1.0 2,245,893 - RR42_RS30610 0.47 +0.5 2,245,893 - RR42_RS30610 0.47 +0.0 2,245,918 + RR42_RS30610 0.49 +0.3 2,245,918 + RR42_RS30610 0.49 +0.8 2,245,918 + RR42_RS30610 0.49 +0.4 2,245,919 - RR42_RS30610 0.49 +0.5 2,245,943 + RR42_RS30610 0.51 +1.0 2,245,944 + RR42_RS30610 0.51 +0.4 2,245,944 - RR42_RS30610 0.51 +0.3 2,245,944 - RR42_RS30610 0.51 +1.2 2,245,944 - RR42_RS30610 0.51 -0.0 2,245,944 - RR42_RS30610 0.51 -0.6 2,245,984 + RR42_RS30610 0.55 -0.3 2,246,060 + RR42_RS30610 0.61 -0.0 2,246,061 - RR42_RS30610 0.61 +0.1 2,246,061 - RR42_RS30610 0.61 -1.2 2,246,061 - RR42_RS30610 0.61 -1.3 2,246,290 + RR42_RS30610 0.80 +0.2 2,246,554 + +0.3 2,246,555 - -0.2 2,246,555 - +0.2 2,246,582 + +0.7 2,246,583 - +0.9 2,246,843 + +0.6 2,246,843 + -1.0 2,246,843 + +1.2 2,246,850 + +0.3 2,246,851 - +1.7 2,246,900 + RR42_RS30620 0.15 -0.7 2,246,900 + RR42_RS30620 0.15 +0.1 2,246,900 + RR42_RS30620 0.15 -0.2 2,246,900 + RR42_RS30620 0.15 +0.3 2,246,900 + RR42_RS30620 0.15 +0.1 2,246,900 + RR42_RS30620 0.15 -0.5 2,246,901 - RR42_RS30620 0.15 -0.6 2,246,932 + RR42_RS30620 0.23 -0.2 2,246,933 - RR42_RS30620 0.23 -0.3 2,246,966 + RR42_RS30620 0.31 -0.4 2,246,967 - RR42_RS30620 0.31 +1.8 2,246,967 - RR42_RS30620 0.31 +0.0 2,246,967 - RR42_RS30620 0.31 -2.1 2,246,967 - RR42_RS30620 0.31 +0.2 2,246,968 + RR42_RS30620 0.31 +0.9 2,246,968 + RR42_RS30620 0.31 -0.9 2,247,059 + RR42_RS30620 0.53 +0.2 2,247,059 + RR42_RS30620 0.53 +1.0 2,247,059 + RR42_RS30620 0.53 +1.1 2,247,060 - RR42_RS30620 0.53 +1.1 2,247,060 - RR42_RS30620 0.53 +0.4 2,247,060 - RR42_RS30620 0.53 -0.8 2,247,079 - RR42_RS30620 0.58 +1.2 2,247,079 - RR42_RS30620 0.58 +0.2 2,247,091 + RR42_RS30620 0.61 +0.8 2,247,092 - RR42_RS30620 0.61 +1.7 2,247,162 + RR42_RS30620 0.78 +0.2 2,247,162 + RR42_RS30620 0.78 +0.1 2,247,162 + RR42_RS30620 0.78 -1.2 2,247,162 + RR42_RS30620 0.78 +0.7 2,247,163 - RR42_RS30620 0.78 +0.7 2,247,163 - RR42_RS30620 0.78 +1.7 2,247,163 - RR42_RS30620 0.78 +0.1 2,247,222 - -0.4 2,247,222 - -1.0 2,247,287 + -0.5 2,247,345 + -0.7 2,247,345 + +0.4 2,247,345 + +1.5 2,247,345 + -0.2 2,247,345 + -0.9 2,247,345 + +1.2 2,247,345 + +0.8 2,247,345 + +0.4 2,247,346 - +0.5 2,247,346 - +0.7 2,247,346 - +0.3 2,247,346 - +1.4 2,247,346 - +0.9 2,247,346 - +1.2 2,247,346 - +0.2 2,247,346 - -0.8 2,247,440 + +0.4 2,247,440 + -2.2 2,247,441 - -0.3 2,247,441 - +0.3 2,247,441 - +1.0 2,247,574 + RR42_RS30625 0.28 +0.9
Or see this region's nucleotide sequence