Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS29500

Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS29495 and RR42_RS29500 are separated by 153 nucleotidesRR42_RS29500 and RR42_RS29505 are separated by 19 nucleotidesRR42_RS29505 and RR42_RS29510 are separated by 138 nucleotides RR42_RS29495: RR42_RS29495 - toxin HipA, at 1,974,112 to 1,975,452 _RS29495 RR42_RS29500: RR42_RS29500 - acyl-CoA dehydrogenase, at 1,975,606 to 1,976,781 _RS29500 RR42_RS29505: RR42_RS29505 - hypothetical protein, at 1,976,801 to 1,977,181 _RS29505 RR42_RS29510: RR42_RS29510 - hypothetical protein, at 1,977,320 to 1,978,909 _RS29510 Position (kb) 1975 1976 1977Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1974.723 kb on + strand, within RR42_RS29495at 1974.724 kb on - strand, within RR42_RS29495at 1974.724 kb on - strand, within RR42_RS29495at 1974.757 kb on - strand, within RR42_RS29495at 1974.769 kb on + strand, within RR42_RS29495at 1974.770 kb on - strand, within RR42_RS29495at 1974.790 kb on - strand, within RR42_RS29495at 1974.931 kb on + strand, within RR42_RS29495at 1974.932 kb on - strand, within RR42_RS29495at 1974.932 kb on - strand, within RR42_RS29495at 1975.123 kb on + strand, within RR42_RS29495at 1975.124 kb on - strand, within RR42_RS29495at 1975.124 kb on - strand, within RR42_RS29495at 1975.124 kb on - strand, within RR42_RS29495at 1975.226 kb on - strand, within RR42_RS29495at 1975.226 kb on - strand, within RR42_RS29495at 1975.451 kb on - strandat 1975.451 kb on - strandat 1975.458 kb on + strandat 1975.458 kb on + strandat 1975.459 kb on - strandat 1975.459 kb on - strandat 1975.459 kb on - strandat 1975.523 kb on + strandat 1975.523 kb on + strandat 1975.524 kb on - strandat 1975.639 kb on + strandat 1975.639 kb on + strandat 1975.640 kb on - strandat 1975.708 kb on + strandat 1975.708 kb on + strandat 1975.708 kb on + strandat 1975.708 kb on + strandat 1975.708 kb on + strandat 1975.709 kb on - strandat 1975.709 kb on - strandat 1975.753 kb on + strand, within RR42_RS29500at 1975.754 kb on - strand, within RR42_RS29500at 1975.831 kb on + strand, within RR42_RS29500at 1975.831 kb on + strand, within RR42_RS29500at 1975.831 kb on + strand, within RR42_RS29500at 1975.832 kb on - strand, within RR42_RS29500at 1975.832 kb on - strand, within RR42_RS29500at 1975.832 kb on - strand, within RR42_RS29500at 1976.071 kb on + strand, within RR42_RS29500at 1976.071 kb on + strand, within RR42_RS29500at 1976.071 kb on + strand, within RR42_RS29500at 1976.071 kb on + strand, within RR42_RS29500at 1976.072 kb on - strand, within RR42_RS29500at 1976.072 kb on - strand, within RR42_RS29500at 1976.380 kb on + strand, within RR42_RS29500at 1976.380 kb on + strand, within RR42_RS29500at 1976.381 kb on - strand, within RR42_RS29500at 1976.381 kb on - strand, within RR42_RS29500at 1976.386 kb on + strand, within RR42_RS29500at 1976.386 kb on + strand, within RR42_RS29500at 1976.387 kb on - strand, within RR42_RS29500at 1976.387 kb on - strand, within RR42_RS29500at 1976.527 kb on + strand, within RR42_RS29500at 1976.528 kb on - strand, within RR42_RS29500at 1976.539 kb on + strand, within RR42_RS29500at 1976.698 kb on + strandat 1976.699 kb on - strandat 1976.825 kb on + strandat 1976.826 kb on - strandat 1976.826 kb on - strandat 1976.826 kb on - strandat 1976.826 kb on - strandat 1976.826 kb on - strandat 1977.002 kb on + strand, within RR42_RS29505at 1977.002 kb on + strand, within RR42_RS29505at 1977.003 kb on - strand, within RR42_RS29505at 1977.151 kb on + strandat 1977.179 kb on + strandat 1977.179 kb on + strandat 1977.179 kb on + strandat 1977.179 kb on + strandat 1977.180 kb on - strandat 1977.180 kb on - strandat 1977.258 kb on + strandat 1977.258 kb on + strandat 1977.259 kb on - strandat 1977.260 kb on + strandat 1977.260 kb on + strandat 1977.261 kb on - strandat 1977.261 kb on - strandat 1977.329 kb on + strandat 1977.454 kb on - strandat 1977.527 kb on + strand, within RR42_RS29510at 1977.527 kb on + strand, within RR42_RS29510at 1977.527 kb on + strand, within RR42_RS29510at 1977.527 kb on + strand, within RR42_RS29510at 1977.527 kb on + strand, within RR42_RS29510at 1977.527 kb on + strand, within RR42_RS29510at 1977.528 kb on - strand, within RR42_RS29510at 1977.528 kb on - strand, within RR42_RS29510at 1977.528 kb on - strand, within RR42_RS29510at 1977.528 kb on - strand, within RR42_RS29510at 1977.528 kb on - strand, within RR42_RS29510at 1977.528 kb on - strand, within RR42_RS29510at 1977.615 kb on + strand, within RR42_RS29510at 1977.615 kb on + strand, within RR42_RS29510at 1977.616 kb on - strand, within RR42_RS29510at 1977.634 kb on - strand, within RR42_RS29510at 1977.637 kb on + strand, within RR42_RS29510at 1977.638 kb on - strand, within RR42_RS29510at 1977.752 kb on + strand, within RR42_RS29510at 1977.770 kb on + strand, within RR42_RS29510at 1977.770 kb on + strand, within RR42_RS29510at 1977.771 kb on - strand, within RR42_RS29510at 1977.776 kb on + strand, within RR42_RS29510at 1977.776 kb on + strand, within RR42_RS29510at 1977.776 kb on + strand, within RR42_RS29510at 1977.777 kb on - strand, within RR42_RS29510at 1977.777 kb on - strand, within RR42_RS29510

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml
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1,974,723 + RR42_RS29495 0.46 +0.2
1,974,724 - RR42_RS29495 0.46 +1.2
1,974,724 - RR42_RS29495 0.46 -0.0
1,974,757 - RR42_RS29495 0.48 +0.4
1,974,769 + RR42_RS29495 0.49 +0.2
1,974,770 - RR42_RS29495 0.49 +0.1
1,974,790 - RR42_RS29495 0.51 -0.1
1,974,931 + RR42_RS29495 0.61 +0.1
1,974,932 - RR42_RS29495 0.61 -0.1
1,974,932 - RR42_RS29495 0.61 -1.2
1,975,123 + RR42_RS29495 0.75 -1.7
1,975,124 - RR42_RS29495 0.75 -0.3
1,975,124 - RR42_RS29495 0.75 +0.4
1,975,124 - RR42_RS29495 0.75 +0.8
1,975,226 - RR42_RS29495 0.83 -1.0
1,975,226 - RR42_RS29495 0.83 +0.2
1,975,451 - +0.8
1,975,451 - +0.7
1,975,458 + -0.5
1,975,458 + -2.3
1,975,459 - -0.5
1,975,459 - -0.4
1,975,459 - -0.1
1,975,523 + -0.3
1,975,523 + -1.9
1,975,524 - -0.2
1,975,639 + +0.5
1,975,639 + +0.5
1,975,640 - +0.5
1,975,708 + -0.1
1,975,708 + +0.1
1,975,708 + +0.7
1,975,708 + +0.1
1,975,708 + +0.8
1,975,709 - -0.7
1,975,709 - -0.8
1,975,753 + RR42_RS29500 0.12 -0.1
1,975,754 - RR42_RS29500 0.13 +0.1
1,975,831 + RR42_RS29500 0.19 +0.2
1,975,831 + RR42_RS29500 0.19 -0.9
1,975,831 + RR42_RS29500 0.19 +1.0
1,975,832 - RR42_RS29500 0.19 +0.1
1,975,832 - RR42_RS29500 0.19 +0.3
1,975,832 - RR42_RS29500 0.19 +0.2
1,976,071 + RR42_RS29500 0.40 -0.4
1,976,071 + RR42_RS29500 0.40 -0.7
1,976,071 + RR42_RS29500 0.40 +0.4
1,976,071 + RR42_RS29500 0.40 +0.1
1,976,072 - RR42_RS29500 0.40 +1.8
1,976,072 - RR42_RS29500 0.40 -0.4
1,976,380 + RR42_RS29500 0.66 +0.3
1,976,380 + RR42_RS29500 0.66 -0.7
1,976,381 - RR42_RS29500 0.66 -0.6
1,976,381 - RR42_RS29500 0.66 -0.9
1,976,386 + RR42_RS29500 0.66 -1.3
1,976,386 + RR42_RS29500 0.66 +0.0
1,976,387 - RR42_RS29500 0.66 -0.8
1,976,387 - RR42_RS29500 0.66 -0.2
1,976,527 + RR42_RS29500 0.78 -3.0
1,976,528 - RR42_RS29500 0.78 +0.5
1,976,539 + RR42_RS29500 0.79 -0.6
1,976,698 + +0.5
1,976,699 - +0.1
1,976,825 + -0.4
1,976,826 - -0.9
1,976,826 - -0.7
1,976,826 - +0.0
1,976,826 - -1.8
1,976,826 - +0.6
1,977,002 + RR42_RS29505 0.53 -2.5
1,977,002 + RR42_RS29505 0.53 -0.6
1,977,003 - RR42_RS29505 0.53 +0.1
1,977,151 + +0.7
1,977,179 + +0.5
1,977,179 + +0.3
1,977,179 + +0.7
1,977,179 + -0.5
1,977,180 - +0.6
1,977,180 - +0.4
1,977,258 + +0.3
1,977,258 + -0.8
1,977,259 - +1.7
1,977,260 + -0.7
1,977,260 + -0.7
1,977,261 - +1.1
1,977,261 - +0.2
1,977,329 + +0.4
1,977,454 - -0.7
1,977,527 + RR42_RS29510 0.13 +0.1
1,977,527 + RR42_RS29510 0.13 -1.3
1,977,527 + RR42_RS29510 0.13 -0.3
1,977,527 + RR42_RS29510 0.13 -0.4
1,977,527 + RR42_RS29510 0.13 +1.4
1,977,527 + RR42_RS29510 0.13 +0.3
1,977,528 - RR42_RS29510 0.13 +0.6
1,977,528 - RR42_RS29510 0.13 -1.7
1,977,528 - RR42_RS29510 0.13 +0.5
1,977,528 - RR42_RS29510 0.13 -1.3
1,977,528 - RR42_RS29510 0.13 -0.3
1,977,528 - RR42_RS29510 0.13 +0.2
1,977,615 + RR42_RS29510 0.19 +0.6
1,977,615 + RR42_RS29510 0.19 -0.4
1,977,616 - RR42_RS29510 0.19 -0.6
1,977,634 - RR42_RS29510 0.20 +0.7
1,977,637 + RR42_RS29510 0.20 +0.9
1,977,638 - RR42_RS29510 0.20 -0.1
1,977,752 + RR42_RS29510 0.27 +1.4
1,977,770 + RR42_RS29510 0.28 +0.7
1,977,770 + RR42_RS29510 0.28 -0.3
1,977,771 - RR42_RS29510 0.28 +2.0
1,977,776 + RR42_RS29510 0.29 -0.7
1,977,776 + RR42_RS29510 0.29 +0.2
1,977,776 + RR42_RS29510 0.29 -1.9
1,977,777 - RR42_RS29510 0.29 -0.1
1,977,777 - RR42_RS29510 0.29 -0.9

Or see this region's nucleotide sequence