Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27650 and RR42_RS27655 are separated by 297 nucleotides RR42_RS27655 and RR42_RS27660 are separated by 121 nucleotides RR42_RS27660 and RR42_RS27665 are separated by 171 nucleotides
RR42_RS27650: RR42_RS27650 - phosphate-binding protein, at 1,566,196 to 1,567,179
_RS27650
RR42_RS27655: RR42_RS27655 - hypothetical protein, at 1,567,477 to 1,568,295
_RS27655
RR42_RS27660: RR42_RS27660 - transcriptional regulator, at 1,568,417 to 1,569,085
_RS27660
RR42_RS27665: RR42_RS27665 - endoribonuclease L-PSP, at 1,569,257 to 1,569,721
_RS27665
Position (kb)
1567
1568
1569 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1566.551 kb on + strand, within RR42_RS27650 at 1566.551 kb on + strand, within RR42_RS27650 at 1566.566 kb on + strand, within RR42_RS27650 at 1566.567 kb on - strand, within RR42_RS27650 at 1566.596 kb on + strand, within RR42_RS27650 at 1566.596 kb on + strand, within RR42_RS27650 at 1566.597 kb on - strand, within RR42_RS27650 at 1566.701 kb on + strand, within RR42_RS27650 at 1566.833 kb on + strand, within RR42_RS27650 at 1566.834 kb on - strand, within RR42_RS27650 at 1566.834 kb on - strand, within RR42_RS27650 at 1566.834 kb on - strand, within RR42_RS27650 at 1566.838 kb on - strand, within RR42_RS27650 at 1567.046 kb on + strand, within RR42_RS27650 at 1567.047 kb on - strand, within RR42_RS27650 at 1567.074 kb on - strand, within RR42_RS27650 at 1567.074 kb on - strand, within RR42_RS27650 at 1567.076 kb on + strand, within RR42_RS27650 at 1567.076 kb on + strand, within RR42_RS27650 at 1567.076 kb on + strand, within RR42_RS27650 at 1567.077 kb on - strand, within RR42_RS27650 at 1567.077 kb on - strand, within RR42_RS27650 at 1567.106 kb on + strand at 1567.106 kb on + strand at 1567.287 kb on - strand at 1567.304 kb on - strand at 1567.304 kb on - strand at 1567.304 kb on - strand at 1567.312 kb on + strand at 1567.312 kb on + strand at 1567.312 kb on + strand at 1567.353 kb on + strand at 1567.354 kb on - strand at 1567.359 kb on - strand at 1567.478 kb on + strand at 1567.652 kb on + strand, within RR42_RS27655 at 1568.072 kb on + strand, within RR42_RS27655 at 1568.073 kb on - strand, within RR42_RS27655 at 1568.073 kb on - strand, within RR42_RS27655 at 1568.142 kb on - strand, within RR42_RS27655 at 1568.315 kb on + strand at 1568.368 kb on - strand at 1568.368 kb on - strand at 1568.418 kb on + strand at 1568.715 kb on + strand, within RR42_RS27660 at 1569.096 kb on + strand at 1569.239 kb on + strand at 1569.239 kb on + strand at 1569.239 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,566,551 + RR42_RS27650 0.36 -0.3 1,566,551 + RR42_RS27650 0.36 -0.3 1,566,566 + RR42_RS27650 0.38 -0.2 1,566,567 - RR42_RS27650 0.38 +0.5 1,566,596 + RR42_RS27650 0.41 +1.9 1,566,596 + RR42_RS27650 0.41 +0.1 1,566,597 - RR42_RS27650 0.41 -0.8 1,566,701 + RR42_RS27650 0.51 +0.4 1,566,833 + RR42_RS27650 0.65 +1.9 1,566,834 - RR42_RS27650 0.65 +0.4 1,566,834 - RR42_RS27650 0.65 +0.3 1,566,834 - RR42_RS27650 0.65 +0.4 1,566,838 - RR42_RS27650 0.65 +0.7 1,567,046 + RR42_RS27650 0.86 -1.3 1,567,047 - RR42_RS27650 0.86 -0.2 1,567,074 - RR42_RS27650 0.89 -1.1 1,567,074 - RR42_RS27650 0.89 +0.7 1,567,076 + RR42_RS27650 0.89 -0.1 1,567,076 + RR42_RS27650 0.89 -0.4 1,567,076 + RR42_RS27650 0.89 -0.7 1,567,077 - RR42_RS27650 0.90 +0.0 1,567,077 - RR42_RS27650 0.90 -0.3 1,567,106 + +0.9 1,567,106 + +0.3 1,567,287 - +0.5 1,567,304 - -1.0 1,567,304 - +0.9 1,567,304 - -0.0 1,567,312 + -0.1 1,567,312 + +0.1 1,567,312 + +0.2 1,567,353 + +0.9 1,567,354 - -0.4 1,567,359 - -0.3 1,567,478 + +0.1 1,567,652 + RR42_RS27655 0.21 +0.0 1,568,072 + RR42_RS27655 0.73 -0.1 1,568,073 - RR42_RS27655 0.73 +1.3 1,568,073 - RR42_RS27655 0.73 -0.0 1,568,142 - RR42_RS27655 0.81 +0.3 1,568,315 + +0.5 1,568,368 - -1.3 1,568,368 - +3.0 1,568,418 + +0.1 1,568,715 + RR42_RS27660 0.45 +2.8 1,569,096 + +0.7 1,569,239 + +0.9 1,569,239 + -0.2 1,569,239 + +0.8
Or see this region's nucleotide sequence