Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26330 and RR42_RS26335 are separated by 48 nucleotides RR42_RS26335 and RR42_RS26340 are separated by 280 nucleotides RR42_RS26340 and RR42_RS26345 are separated by 137 nucleotides
RR42_RS26330: RR42_RS26330 - hypothetical protein, at 1,265,926 to 1,266,414
_RS26330
RR42_RS26335: RR42_RS26335 - hypothetical protein, at 1,266,463 to 1,266,927
_RS26335
RR42_RS26340: RR42_RS26340 - metallophosphoesterase, at 1,267,208 to 1,267,969
_RS26340
RR42_RS26345: RR42_RS26345 - hypothetical protein, at 1,268,107 to 1,268,913
_RS26345
Position (kb) 1267
1268 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1266.222 kb on - strand, within RR42_RS26330 at 1266.233 kb on + strand, within RR42_RS26330 at 1266.234 kb on - strand, within RR42_RS26330 at 1266.234 kb on - strand, within RR42_RS26330 at 1266.234 kb on - strand, within RR42_RS26330 at 1266.234 kb on - strand, within RR42_RS26330 at 1266.343 kb on + strand, within RR42_RS26330 at 1266.343 kb on + strand, within RR42_RS26330 at 1266.388 kb on - strand at 1266.438 kb on + strand at 1266.438 kb on + strand at 1266.438 kb on + strand at 1266.439 kb on - strand at 1266.439 kb on - strand at 1266.439 kb on - strand at 1266.628 kb on + strand, within RR42_RS26335 at 1266.629 kb on - strand, within RR42_RS26335 at 1266.677 kb on + strand, within RR42_RS26335 at 1266.755 kb on + strand, within RR42_RS26335 at 1266.756 kb on - strand, within RR42_RS26335 at 1266.756 kb on - strand, within RR42_RS26335 at 1266.918 kb on + strand at 1266.918 kb on + strand at 1266.919 kb on - strand at 1266.933 kb on + strand at 1266.933 kb on + strand at 1266.933 kb on + strand at 1266.934 kb on - strand at 1266.934 kb on - strand at 1267.063 kb on + strand at 1267.063 kb on + strand at 1267.064 kb on - strand at 1267.064 kb on - strand at 1267.064 kb on - strand at 1267.064 kb on - strand at 1267.064 kb on - strand at 1267.098 kb on + strand at 1267.099 kb on - strand at 1267.107 kb on - strand at 1267.269 kb on + strand at 1267.270 kb on - strand at 1267.733 kb on + strand, within RR42_RS26340 at 1267.733 kb on + strand, within RR42_RS26340 at 1267.733 kb on + strand, within RR42_RS26340 at 1267.734 kb on - strand, within RR42_RS26340 at 1267.818 kb on + strand, within RR42_RS26340 at 1267.819 kb on - strand, within RR42_RS26340 at 1267.819 kb on - strand, within RR42_RS26340 at 1267.929 kb on + strand at 1267.929 kb on + strand at 1267.930 kb on - strand at 1268.058 kb on + strand at 1268.058 kb on + strand at 1268.059 kb on - strand at 1268.059 kb on - strand at 1268.059 kb on - strand at 1268.063 kb on + strand at 1268.063 kb on + strand at 1268.063 kb on + strand at 1268.063 kb on + strand at 1268.063 kb on + strand at 1268.064 kb on - strand at 1268.064 kb on - strand at 1268.064 kb on - strand at 1268.070 kb on - strand at 1268.070 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,266,222 - RR42_RS26330 0.61 -0.8 1,266,233 + RR42_RS26330 0.63 +0.0 1,266,234 - RR42_RS26330 0.63 -0.3 1,266,234 - RR42_RS26330 0.63 -1.3 1,266,234 - RR42_RS26330 0.63 -0.1 1,266,234 - RR42_RS26330 0.63 +1.4 1,266,343 + RR42_RS26330 0.85 +1.0 1,266,343 + RR42_RS26330 0.85 +0.3 1,266,388 - -1.2 1,266,438 + +0.5 1,266,438 + +0.9 1,266,438 + -0.6 1,266,439 - +0.5 1,266,439 - +1.0 1,266,439 - +0.4 1,266,628 + RR42_RS26335 0.35 +0.3 1,266,629 - RR42_RS26335 0.36 -2.0 1,266,677 + RR42_RS26335 0.46 +0.3 1,266,755 + RR42_RS26335 0.63 -0.1 1,266,756 - RR42_RS26335 0.63 +0.4 1,266,756 - RR42_RS26335 0.63 -0.3 1,266,918 + +1.6 1,266,918 + +0.5 1,266,919 - +1.1 1,266,933 + -0.5 1,266,933 + -1.4 1,266,933 + -0.0 1,266,934 - +0.3 1,266,934 - -0.3 1,267,063 + -0.7 1,267,063 + -0.9 1,267,064 - +0.9 1,267,064 - -0.5 1,267,064 - -0.4 1,267,064 - -0.1 1,267,064 - +0.3 1,267,098 + +1.3 1,267,099 - +0.1 1,267,107 - -0.1 1,267,269 + +1.0 1,267,270 - -0.0 1,267,733 + RR42_RS26340 0.69 +0.1 1,267,733 + RR42_RS26340 0.69 -2.5 1,267,733 + RR42_RS26340 0.69 +0.6 1,267,734 - RR42_RS26340 0.69 +0.8 1,267,818 + RR42_RS26340 0.80 +0.8 1,267,819 - RR42_RS26340 0.80 +0.6 1,267,819 - RR42_RS26340 0.80 -1.2 1,267,929 + -1.0 1,267,929 + -0.0 1,267,930 - -0.1 1,268,058 + -0.1 1,268,058 + -0.0 1,268,059 - +0.6 1,268,059 - +0.3 1,268,059 - -0.1 1,268,063 + +0.3 1,268,063 + +0.6 1,268,063 + +0.7 1,268,063 + +0.1 1,268,063 + -1.0 1,268,064 - +0.4 1,268,064 - +1.8 1,268,064 - +0.1 1,268,070 - -0.0 1,268,070 - -2.6
Or see this region's nucleotide sequence