Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25585 and RR42_RS25590 are separated by 499 nucleotides RR42_RS25590 and RR42_RS25595 are separated by 84 nucleotides
RR42_RS25585: RR42_RS25585 - membrane protein, at 1,084,229 to 1,085,392
_RS25585
RR42_RS25590: RR42_RS25590 - glucose 1-dehydrogenase, at 1,085,892 to 1,086,635
_RS25590
RR42_RS25595: RR42_RS25595 - alpha/beta hydrolase, at 1,086,720 to 1,087,583
_RS25595
Position (kb)
1085
1086
1087 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1084.920 kb on - strand, within RR42_RS25585 at 1084.920 kb on - strand, within RR42_RS25585 at 1084.989 kb on - strand, within RR42_RS25585 at 1084.989 kb on - strand, within RR42_RS25585 at 1084.995 kb on - strand, within RR42_RS25585 at 1085.075 kb on + strand, within RR42_RS25585 at 1085.076 kb on - strand, within RR42_RS25585 at 1085.078 kb on + strand, within RR42_RS25585 at 1085.097 kb on - strand, within RR42_RS25585 at 1085.133 kb on - strand, within RR42_RS25585 at 1085.138 kb on + strand, within RR42_RS25585 at 1085.139 kb on - strand, within RR42_RS25585 at 1085.210 kb on + strand, within RR42_RS25585 at 1085.227 kb on + strand, within RR42_RS25585 at 1085.237 kb on + strand, within RR42_RS25585 at 1085.238 kb on - strand, within RR42_RS25585 at 1085.328 kb on - strand at 1085.447 kb on - strand at 1085.447 kb on - strand at 1085.612 kb on - strand at 1085.683 kb on + strand at 1085.723 kb on - strand at 1085.969 kb on - strand, within RR42_RS25590 at 1085.989 kb on + strand, within RR42_RS25590 at 1085.990 kb on - strand, within RR42_RS25590 at 1085.990 kb on - strand, within RR42_RS25590 at 1086.281 kb on - strand, within RR42_RS25590 at 1086.281 kb on - strand, within RR42_RS25590 at 1086.547 kb on + strand, within RR42_RS25590 at 1086.655 kb on + strand at 1086.800 kb on - strand at 1086.842 kb on + strand, within RR42_RS25595 at 1087.075 kb on + strand, within RR42_RS25595 at 1087.076 kb on - strand, within RR42_RS25595 at 1087.079 kb on - strand, within RR42_RS25595 at 1087.079 kb on - strand, within RR42_RS25595 at 1087.316 kb on - strand, within RR42_RS25595 at 1087.525 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,084,920 - RR42_RS25585 0.59 -1.5 1,084,920 - RR42_RS25585 0.59 -1.2 1,084,989 - RR42_RS25585 0.65 -0.3 1,084,989 - RR42_RS25585 0.65 +0.4 1,084,995 - RR42_RS25585 0.66 -0.2 1,085,075 + RR42_RS25585 0.73 +0.6 1,085,076 - RR42_RS25585 0.73 +1.3 1,085,078 + RR42_RS25585 0.73 +0.9 1,085,097 - RR42_RS25585 0.75 -0.3 1,085,133 - RR42_RS25585 0.78 +0.0 1,085,138 + RR42_RS25585 0.78 +0.3 1,085,139 - RR42_RS25585 0.78 -2.8 1,085,210 + RR42_RS25585 0.84 +0.1 1,085,227 + RR42_RS25585 0.86 -0.6 1,085,237 + RR42_RS25585 0.87 -1.4 1,085,238 - RR42_RS25585 0.87 -1.6 1,085,328 - -0.7 1,085,447 - -0.3 1,085,447 - -0.9 1,085,612 - +0.3 1,085,683 + -0.2 1,085,723 - -0.3 1,085,969 - RR42_RS25590 0.10 +0.0 1,085,989 + RR42_RS25590 0.13 -0.7 1,085,990 - RR42_RS25590 0.13 -0.4 1,085,990 - RR42_RS25590 0.13 -2.8 1,086,281 - RR42_RS25590 0.52 -0.8 1,086,281 - RR42_RS25590 0.52 +0.3 1,086,547 + RR42_RS25590 0.88 -0.7 1,086,655 + -1.1 1,086,800 - -0.6 1,086,842 + RR42_RS25595 0.14 +0.1 1,087,075 + RR42_RS25595 0.41 +0.3 1,087,076 - RR42_RS25595 0.41 +0.0 1,087,079 - RR42_RS25595 0.42 -1.0 1,087,079 - RR42_RS25595 0.42 +1.1 1,087,316 - RR42_RS25595 0.69 +0.6 1,087,525 - +0.4
Or see this region's nucleotide sequence