Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25550 and RR42_RS25555 are separated by 181 nucleotides RR42_RS25555 and RR42_RS25560 are separated by 137 nucleotides RR42_RS25560 and RR42_RS25565 are separated by 3 nucleotides
RR42_RS25550: RR42_RS25550 - Crp/Fnr family transcriptional regulator, at 1,076,976 to 1,077,665
_RS25550
RR42_RS25555: RR42_RS25555 - HD family phosphohydrolase, at 1,077,847 to 1,078,080
_RS25555
RR42_RS25560: RR42_RS25560 - MFS transporter, at 1,078,218 to 1,079,561
_RS25560
RR42_RS25565: RR42_RS25565 - alpha/beta hydrolase, at 1,079,565 to 1,080,719
_RS25565
Position (kb)
1078
1079
1080 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1077.609 kb on + strand at 1077.609 kb on + strand at 1077.610 kb on - strand at 1077.698 kb on + strand at 1077.699 kb on - strand at 1077.748 kb on - strand at 1077.784 kb on - strand at 1077.784 kb on - strand at 1077.784 kb on - strand at 1077.856 kb on + strand at 1077.857 kb on - strand at 1077.919 kb on + strand, within RR42_RS25555 at 1077.949 kb on + strand, within RR42_RS25555 at 1077.949 kb on + strand, within RR42_RS25555 at 1077.950 kb on - strand, within RR42_RS25555 at 1078.048 kb on + strand, within RR42_RS25555 at 1078.049 kb on - strand, within RR42_RS25555 at 1078.049 kb on - strand, within RR42_RS25555 at 1078.049 kb on - strand, within RR42_RS25555 at 1078.156 kb on + strand at 1078.156 kb on + strand at 1078.156 kb on + strand at 1078.157 kb on - strand at 1078.158 kb on + strand at 1078.159 kb on - strand at 1078.217 kb on + strand at 1078.217 kb on + strand at 1078.238 kb on - strand at 1078.238 kb on - strand at 1078.254 kb on + strand at 1078.299 kb on + strand at 1078.311 kb on + strand at 1078.311 kb on + strand at 1078.311 kb on + strand at 1078.312 kb on - strand at 1078.312 kb on - strand at 1078.313 kb on + strand at 1078.383 kb on + strand, within RR42_RS25560 at 1078.416 kb on + strand, within RR42_RS25560 at 1078.532 kb on + strand, within RR42_RS25560 at 1078.608 kb on + strand, within RR42_RS25560 at 1078.795 kb on + strand, within RR42_RS25560 at 1078.796 kb on - strand, within RR42_RS25560 at 1078.810 kb on - strand, within RR42_RS25560 at 1079.027 kb on + strand, within RR42_RS25560 at 1079.028 kb on - strand, within RR42_RS25560 at 1079.092 kb on - strand, within RR42_RS25560 at 1079.223 kb on + strand, within RR42_RS25560 at 1079.223 kb on + strand, within RR42_RS25560 at 1079.224 kb on - strand, within RR42_RS25560 at 1079.224 kb on - strand, within RR42_RS25560 at 1079.359 kb on + strand, within RR42_RS25560 at 1079.388 kb on + strand, within RR42_RS25560 at 1079.389 kb on - strand, within RR42_RS25560 at 1079.395 kb on + strand, within RR42_RS25560 at 1079.772 kb on + strand, within RR42_RS25565 at 1079.772 kb on + strand, within RR42_RS25565 at 1079.773 kb on - strand, within RR42_RS25565 at 1079.879 kb on - strand, within RR42_RS25565 at 1080.139 kb on - strand, within RR42_RS25565 at 1080.139 kb on - strand, within RR42_RS25565 at 1080.139 kb on - strand, within RR42_RS25565 at 1080.142 kb on - strand, within RR42_RS25565 at 1080.142 kb on - strand, within RR42_RS25565 at 1080.211 kb on - strand, within RR42_RS25565 at 1080.213 kb on + strand, within RR42_RS25565 at 1080.214 kb on - strand, within RR42_RS25565 at 1080.300 kb on + strand, within RR42_RS25565 at 1080.301 kb on - strand, within RR42_RS25565 at 1080.303 kb on + strand, within RR42_RS25565 at 1080.408 kb on + strand, within RR42_RS25565 at 1080.410 kb on + strand, within RR42_RS25565 at 1080.457 kb on - strand, within RR42_RS25565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,077,609 + +0.4 1,077,609 + -0.1 1,077,610 - +1.3 1,077,698 + +0.6 1,077,699 - +0.1 1,077,748 - +0.3 1,077,784 - -0.4 1,077,784 - -3.5 1,077,784 - -1.5 1,077,856 + +0.4 1,077,857 - -0.8 1,077,919 + RR42_RS25555 0.31 -0.3 1,077,949 + RR42_RS25555 0.44 -1.4 1,077,949 + RR42_RS25555 0.44 +0.7 1,077,950 - RR42_RS25555 0.44 -0.1 1,078,048 + RR42_RS25555 0.86 +0.0 1,078,049 - RR42_RS25555 0.86 -0.4 1,078,049 - RR42_RS25555 0.86 +0.3 1,078,049 - RR42_RS25555 0.86 +0.2 1,078,156 + +0.3 1,078,156 + +0.6 1,078,156 + -0.6 1,078,157 - +1.4 1,078,158 + -1.9 1,078,159 - +0.8 1,078,217 + +0.5 1,078,217 + +0.0 1,078,238 - -0.1 1,078,238 - -0.0 1,078,254 + -0.5 1,078,299 + +1.4 1,078,311 + +0.1 1,078,311 + -1.6 1,078,311 + -1.2 1,078,312 - +0.9 1,078,312 - +1.4 1,078,313 + +3.1 1,078,383 + RR42_RS25560 0.12 -0.3 1,078,416 + RR42_RS25560 0.15 -2.4 1,078,532 + RR42_RS25560 0.23 +1.2 1,078,608 + RR42_RS25560 0.29 -0.1 1,078,795 + RR42_RS25560 0.43 -0.1 1,078,796 - RR42_RS25560 0.43 -1.0 1,078,810 - RR42_RS25560 0.44 -1.4 1,079,027 + RR42_RS25560 0.60 +1.1 1,079,028 - RR42_RS25560 0.60 +0.4 1,079,092 - RR42_RS25560 0.65 -0.3 1,079,223 + RR42_RS25560 0.75 -0.5 1,079,223 + RR42_RS25560 0.75 +1.4 1,079,224 - RR42_RS25560 0.75 -2.4 1,079,224 - RR42_RS25560 0.75 +1.2 1,079,359 + RR42_RS25560 0.85 -0.7 1,079,388 + RR42_RS25560 0.87 +0.9 1,079,389 - RR42_RS25560 0.87 -0.0 1,079,395 + RR42_RS25560 0.88 +0.4 1,079,772 + RR42_RS25565 0.18 -1.1 1,079,772 + RR42_RS25565 0.18 -0.4 1,079,773 - RR42_RS25565 0.18 -0.1 1,079,879 - RR42_RS25565 0.27 -1.8 1,080,139 - RR42_RS25565 0.50 +0.2 1,080,139 - RR42_RS25565 0.50 +0.3 1,080,139 - RR42_RS25565 0.50 -0.5 1,080,142 - RR42_RS25565 0.50 -0.8 1,080,142 - RR42_RS25565 0.50 -0.3 1,080,211 - RR42_RS25565 0.56 +0.4 1,080,213 + RR42_RS25565 0.56 -0.1 1,080,214 - RR42_RS25565 0.56 +0.9 1,080,300 + RR42_RS25565 0.64 -2.1 1,080,301 - RR42_RS25565 0.64 -0.2 1,080,303 + RR42_RS25565 0.64 -1.0 1,080,408 + RR42_RS25565 0.73 -0.6 1,080,410 + RR42_RS25565 0.73 -0.2 1,080,457 - RR42_RS25565 0.77 -0.1
Or see this region's nucleotide sequence