Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25515 and RR42_RS25520 are separated by 50 nucleotides RR42_RS25520 and RR42_RS25525 overlap by 4 nucleotides RR42_RS25525 and RR42_RS25530 are separated by 158 nucleotides RR42_RS25530 and RR42_RS25535 are separated by 132 nucleotides
RR42_RS25515: RR42_RS25515 - AMP-dependent synthetase, at 1,069,867 to 1,071,327
_RS25515
RR42_RS25520: RR42_RS25520 - alpha/beta hydrolase, at 1,071,378 to 1,072,310
_RS25520
RR42_RS25525: RR42_RS25525 - enoyl-CoA hydratase, at 1,072,307 to 1,073,281
_RS25525
RR42_RS25530: RR42_RS25530 - TetR family transcriptional regulator, at 1,073,440 to 1,074,090
_RS25530
RR42_RS25535: RR42_RS25535 - TetR family transcriptional regulator, at 1,074,223 to 1,074,834
_RS25535
Position (kb)
1072
1073
1074 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1071.340 kb on + strand at 1071.340 kb on + strand at 1071.341 kb on - strand at 1071.344 kb on + strand at 1071.454 kb on + strand at 1071.455 kb on - strand at 1071.685 kb on + strand, within RR42_RS25520 at 1071.807 kb on - strand, within RR42_RS25520 at 1071.840 kb on - strand, within RR42_RS25520 at 1071.942 kb on - strand, within RR42_RS25520 at 1072.017 kb on + strand, within RR42_RS25520 at 1072.018 kb on - strand, within RR42_RS25520 at 1072.079 kb on - strand, within RR42_RS25520 at 1072.079 kb on - strand, within RR42_RS25520 at 1072.229 kb on - strand at 1072.365 kb on + strand at 1072.522 kb on - strand, within RR42_RS25525 at 1072.573 kb on - strand, within RR42_RS25525 at 1072.760 kb on + strand, within RR42_RS25525 at 1072.760 kb on + strand, within RR42_RS25525 at 1072.761 kb on - strand, within RR42_RS25525 at 1072.807 kb on + strand, within RR42_RS25525 at 1073.028 kb on - strand, within RR42_RS25525 at 1073.111 kb on + strand, within RR42_RS25525 at 1073.112 kb on - strand, within RR42_RS25525 at 1073.243 kb on + strand at 1073.243 kb on + strand at 1073.243 kb on + strand at 1073.244 kb on - strand at 1073.409 kb on + strand at 1073.439 kb on + strand at 1073.440 kb on - strand at 1073.440 kb on - strand at 1073.451 kb on + strand at 1073.578 kb on + strand, within RR42_RS25530 at 1073.578 kb on + strand, within RR42_RS25530 at 1073.578 kb on + strand, within RR42_RS25530 at 1073.579 kb on - strand, within RR42_RS25530 at 1073.693 kb on + strand, within RR42_RS25530 at 1073.693 kb on + strand, within RR42_RS25530 at 1073.746 kb on + strand, within RR42_RS25530 at 1073.782 kb on + strand, within RR42_RS25530 at 1073.782 kb on + strand, within RR42_RS25530 at 1073.783 kb on - strand, within RR42_RS25530 at 1073.783 kb on - strand, within RR42_RS25530 at 1073.783 kb on - strand, within RR42_RS25530 at 1073.783 kb on - strand, within RR42_RS25530 at 1073.784 kb on + strand, within RR42_RS25530 at 1073.854 kb on + strand, within RR42_RS25530 at 1073.855 kb on - strand, within RR42_RS25530 at 1073.933 kb on + strand, within RR42_RS25530 at 1073.933 kb on + strand, within RR42_RS25530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,071,340 + +0.5 1,071,340 + +1.1 1,071,341 - -0.2 1,071,344 + +0.1 1,071,454 + +0.2 1,071,455 - -0.3 1,071,685 + RR42_RS25520 0.33 -0.4 1,071,807 - RR42_RS25520 0.46 +0.0 1,071,840 - RR42_RS25520 0.50 +0.8 1,071,942 - RR42_RS25520 0.60 -0.1 1,072,017 + RR42_RS25520 0.68 +0.3 1,072,018 - RR42_RS25520 0.69 +0.9 1,072,079 - RR42_RS25520 0.75 +0.2 1,072,079 - RR42_RS25520 0.75 +0.7 1,072,229 - +0.9 1,072,365 + +1.6 1,072,522 - RR42_RS25525 0.22 +0.5 1,072,573 - RR42_RS25525 0.27 -0.2 1,072,760 + RR42_RS25525 0.46 -0.1 1,072,760 + RR42_RS25525 0.46 -0.1 1,072,761 - RR42_RS25525 0.47 +2.2 1,072,807 + RR42_RS25525 0.51 -0.4 1,073,028 - RR42_RS25525 0.74 -0.0 1,073,111 + RR42_RS25525 0.82 +0.2 1,073,112 - RR42_RS25525 0.83 +0.6 1,073,243 + -2.5 1,073,243 + -0.9 1,073,243 + +0.5 1,073,244 - +0.1 1,073,409 + -0.6 1,073,439 + -0.8 1,073,440 - -2.0 1,073,440 - +0.4 1,073,451 + +0.1 1,073,578 + RR42_RS25530 0.21 +1.9 1,073,578 + RR42_RS25530 0.21 -1.4 1,073,578 + RR42_RS25530 0.21 -1.8 1,073,579 - RR42_RS25530 0.21 -2.2 1,073,693 + RR42_RS25530 0.39 -0.1 1,073,693 + RR42_RS25530 0.39 -0.3 1,073,746 + RR42_RS25530 0.47 -0.3 1,073,782 + RR42_RS25530 0.53 -1.4 1,073,782 + RR42_RS25530 0.53 -0.8 1,073,783 - RR42_RS25530 0.53 -2.1 1,073,783 - RR42_RS25530 0.53 -1.4 1,073,783 - RR42_RS25530 0.53 -1.9 1,073,783 - RR42_RS25530 0.53 -1.8 1,073,784 + RR42_RS25530 0.53 +0.9 1,073,854 + RR42_RS25530 0.64 -1.2 1,073,855 - RR42_RS25530 0.64 -3.0 1,073,933 + RR42_RS25530 0.76 -2.0 1,073,933 + RR42_RS25530 0.76 -0.4
Or see this region's nucleotide sequence