Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25280 and RR42_RS25285 overlap by 4 nucleotides RR42_RS25285 and RR42_RS25290 are separated by 41 nucleotides
RR42_RS25280: RR42_RS25280 - 3-hydroxy-3-methylglutaryl-CoA lyase, at 1,011,681 to 1,012,652
_RS25280
RR42_RS25285: RR42_RS25285 - CoA-transferase, at 1,012,649 to 1,013,800
_RS25285
RR42_RS25290: RR42_RS25290 - ABC transporter substrate-binding protein, at 1,013,842 to 1,014,852
_RS25290
Position (kb)
1012
1013
1014 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1011.671 kb on + strand at 1011.672 kb on - strand at 1011.690 kb on - strand at 1011.690 kb on - strand at 1011.690 kb on - strand at 1011.883 kb on + strand, within RR42_RS25280 at 1011.884 kb on - strand, within RR42_RS25280 at 1011.928 kb on + strand, within RR42_RS25280 at 1012.048 kb on + strand, within RR42_RS25280 at 1012.462 kb on - strand, within RR42_RS25280 at 1012.643 kb on + strand at 1012.643 kb on + strand at 1012.977 kb on - strand, within RR42_RS25285 at 1013.012 kb on + strand, within RR42_RS25285 at 1013.012 kb on + strand, within RR42_RS25285 at 1013.013 kb on - strand, within RR42_RS25285 at 1013.013 kb on - strand, within RR42_RS25285 at 1013.045 kb on + strand, within RR42_RS25285 at 1013.046 kb on - strand, within RR42_RS25285 at 1013.046 kb on - strand, within RR42_RS25285 at 1013.099 kb on + strand, within RR42_RS25285 at 1013.100 kb on - strand, within RR42_RS25285 at 1013.213 kb on - strand, within RR42_RS25285 at 1013.289 kb on - strand, within RR42_RS25285 at 1013.346 kb on + strand, within RR42_RS25285 at 1013.346 kb on + strand, within RR42_RS25285 at 1013.349 kb on - strand, within RR42_RS25285 at 1013.349 kb on - strand, within RR42_RS25285 at 1013.349 kb on - strand, within RR42_RS25285 at 1013.465 kb on + strand, within RR42_RS25285 at 1013.465 kb on + strand, within RR42_RS25285 at 1013.466 kb on - strand, within RR42_RS25285 at 1013.585 kb on + strand, within RR42_RS25285 at 1013.585 kb on + strand, within RR42_RS25285 at 1013.586 kb on - strand, within RR42_RS25285 at 1013.784 kb on + strand at 1013.784 kb on + strand at 1013.818 kb on - strand at 1014.177 kb on + strand, within RR42_RS25290 at 1014.177 kb on + strand, within RR42_RS25290 at 1014.190 kb on + strand, within RR42_RS25290 at 1014.190 kb on + strand, within RR42_RS25290 at 1014.191 kb on - strand, within RR42_RS25290 at 1014.430 kb on + strand, within RR42_RS25290 at 1014.430 kb on + strand, within RR42_RS25290 at 1014.431 kb on - strand, within RR42_RS25290 at 1014.431 kb on - strand, within RR42_RS25290 at 1014.488 kb on + strand, within RR42_RS25290 at 1014.488 kb on + strand, within RR42_RS25290 at 1014.489 kb on - strand, within RR42_RS25290 at 1014.489 kb on - strand, within RR42_RS25290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 1,011,671 + -0.5 1,011,672 - -0.2 1,011,690 - -0.4 1,011,690 - +0.1 1,011,690 - +1.2 1,011,883 + RR42_RS25280 0.21 -2.0 1,011,884 - RR42_RS25280 0.21 +0.0 1,011,928 + RR42_RS25280 0.25 +0.4 1,012,048 + RR42_RS25280 0.38 +1.8 1,012,462 - RR42_RS25280 0.80 -0.5 1,012,643 + +0.3 1,012,643 + +0.6 1,012,977 - RR42_RS25285 0.28 -1.0 1,013,012 + RR42_RS25285 0.32 -0.1 1,013,012 + RR42_RS25285 0.32 -1.4 1,013,013 - RR42_RS25285 0.32 +0.0 1,013,013 - RR42_RS25285 0.32 +1.7 1,013,045 + RR42_RS25285 0.34 -2.1 1,013,046 - RR42_RS25285 0.34 +1.2 1,013,046 - RR42_RS25285 0.34 -2.4 1,013,099 + RR42_RS25285 0.39 -0.3 1,013,100 - RR42_RS25285 0.39 +0.1 1,013,213 - RR42_RS25285 0.49 +0.1 1,013,289 - RR42_RS25285 0.56 -0.4 1,013,346 + RR42_RS25285 0.61 -1.0 1,013,346 + RR42_RS25285 0.61 +0.6 1,013,349 - RR42_RS25285 0.61 -0.4 1,013,349 - RR42_RS25285 0.61 -3.4 1,013,349 - RR42_RS25285 0.61 +1.1 1,013,465 + RR42_RS25285 0.71 -0.2 1,013,465 + RR42_RS25285 0.71 -0.2 1,013,466 - RR42_RS25285 0.71 -0.5 1,013,585 + RR42_RS25285 0.81 -0.8 1,013,585 + RR42_RS25285 0.81 -0.2 1,013,586 - RR42_RS25285 0.81 -1.9 1,013,784 + +1.1 1,013,784 + -1.2 1,013,818 - +0.9 1,014,177 + RR42_RS25290 0.33 -2.2 1,014,177 + RR42_RS25290 0.33 +1.5 1,014,190 + RR42_RS25290 0.34 -0.5 1,014,190 + RR42_RS25290 0.34 +0.6 1,014,191 - RR42_RS25290 0.35 +0.1 1,014,430 + RR42_RS25290 0.58 -0.1 1,014,430 + RR42_RS25290 0.58 +0.7 1,014,431 - RR42_RS25290 0.58 -0.1 1,014,431 - RR42_RS25290 0.58 -0.4 1,014,488 + RR42_RS25290 0.64 +0.3 1,014,488 + RR42_RS25290 0.64 -1.0 1,014,489 - RR42_RS25290 0.64 -1.4 1,014,489 - RR42_RS25290 0.64 -0.7
Or see this region's nucleotide sequence