Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24550 and RR42_RS24555 are separated by 818 nucleotides RR42_RS24555 and RR42_RS24560 are separated by 35 nucleotides
RR42_RS24550: RR42_RS24550 - hypothetical protein, at 839,922 to 840,104
_RS24550
RR42_RS24555: RR42_RS24555 - succinate-semialdehyde dehydrogenase, at 840,923 to 842,407
_RS24555
RR42_RS24560: RR42_RS24560 - alcohol dehydrogenase, at 842,443 to 843,612
_RS24560
Position (kb) 840
841
842
843 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 839.971 kb on - strand, within RR42_RS24550 at 840.064 kb on - strand, within RR42_RS24550 at 840.092 kb on + strand at 840.217 kb on - strand at 840.226 kb on - strand at 840.256 kb on + strand at 840.257 kb on - strand at 840.280 kb on + strand at 840.280 kb on + strand at 840.296 kb on - strand at 840.302 kb on + strand at 840.345 kb on + strand at 840.366 kb on + strand at 840.366 kb on + strand at 840.366 kb on + strand at 840.367 kb on - strand at 840.370 kb on - strand at 840.464 kb on + strand at 840.465 kb on - strand at 840.544 kb on - strand at 840.590 kb on + strand at 840.739 kb on + strand at 840.739 kb on + strand at 840.740 kb on - strand at 840.796 kb on + strand at 840.796 kb on + strand at 840.796 kb on + strand at 840.797 kb on - strand at 840.797 kb on - strand at 840.797 kb on - strand at 840.797 kb on - strand at 840.797 kb on - strand at 840.844 kb on - strand at 840.844 kb on - strand at 840.864 kb on + strand at 840.864 kb on + strand at 840.865 kb on - strand at 840.866 kb on + strand at 840.866 kb on + strand at 840.866 kb on + strand at 840.866 kb on + strand at 840.866 kb on + strand at 840.866 kb on + strand at 840.867 kb on - strand at 840.867 kb on - strand at 840.867 kb on - strand at 840.907 kb on + strand at 840.907 kb on + strand at 840.907 kb on + strand at 840.907 kb on + strand at 840.907 kb on + strand at 840.907 kb on + strand at 840.907 kb on + strand at 840.908 kb on - strand at 840.908 kb on - strand at 840.908 kb on - strand at 840.922 kb on - strand at 840.922 kb on - strand at 840.935 kb on - strand at 840.946 kb on + strand at 840.947 kb on - strand at 840.947 kb on - strand at 841.177 kb on + strand, within RR42_RS24555 at 841.177 kb on + strand, within RR42_RS24555 at 841.177 kb on + strand, within RR42_RS24555 at 841.178 kb on - strand, within RR42_RS24555 at 841.337 kb on + strand, within RR42_RS24555 at 841.337 kb on + strand, within RR42_RS24555 at 841.338 kb on - strand, within RR42_RS24555 at 841.338 kb on - strand, within RR42_RS24555 at 841.338 kb on - strand, within RR42_RS24555 at 841.490 kb on - strand, within RR42_RS24555 at 841.862 kb on + strand, within RR42_RS24555 at 841.862 kb on + strand, within RR42_RS24555 at 841.863 kb on - strand, within RR42_RS24555 at 842.130 kb on + strand, within RR42_RS24555 at 842.192 kb on + strand, within RR42_RS24555 at 842.192 kb on + strand, within RR42_RS24555 at 842.213 kb on + strand, within RR42_RS24555 at 842.213 kb on + strand, within RR42_RS24555 at 842.261 kb on + strand at 842.372 kb on + strand at 842.373 kb on - strand at 842.388 kb on - strand at 842.388 kb on - strand at 842.388 kb on - strand at 842.466 kb on - strand at 842.818 kb on + strand, within RR42_RS24560 at 842.818 kb on + strand, within RR42_RS24560 at 842.818 kb on + strand, within RR42_RS24560 at 842.818 kb on + strand, within RR42_RS24560 at 842.818 kb on + strand, within RR42_RS24560 at 842.819 kb on - strand, within RR42_RS24560 at 842.819 kb on - strand, within RR42_RS24560 at 842.902 kb on - strand, within RR42_RS24560 at 842.902 kb on - strand, within RR42_RS24560 at 842.960 kb on - strand, within RR42_RS24560 at 843.062 kb on - strand, within RR42_RS24560 at 843.062 kb on - strand, within RR42_RS24560 at 843.062 kb on - strand, within RR42_RS24560 at 843.125 kb on + strand, within RR42_RS24560 at 843.141 kb on - strand, within RR42_RS24560 at 843.257 kb on - strand, within RR42_RS24560
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 839,971 - RR42_RS24550 0.27 -1.3 840,064 - RR42_RS24550 0.78 -0.7 840,092 + -0.4 840,217 - -1.1 840,226 - +2.3 840,256 + +1.0 840,257 - -0.4 840,280 + -1.0 840,280 + +0.4 840,296 - +0.2 840,302 + -0.1 840,345 + -0.6 840,366 + +0.7 840,366 + +0.7 840,366 + -0.1 840,367 - -1.0 840,370 - -2.8 840,464 + -0.5 840,465 - -0.3 840,544 - -1.2 840,590 + -0.1 840,739 + -0.3 840,739 + -0.6 840,740 - +0.4 840,796 + +0.4 840,796 + -0.6 840,796 + +0.8 840,797 - -0.0 840,797 - +0.1 840,797 - -0.2 840,797 - -0.1 840,797 - +0.5 840,844 - -0.4 840,844 - +0.9 840,864 + +0.7 840,864 + -0.0 840,865 - -0.1 840,866 + -1.5 840,866 + -1.2 840,866 + +1.3 840,866 + -0.2 840,866 + -1.4 840,866 + +1.3 840,867 - -0.7 840,867 - +0.4 840,867 - -0.8 840,907 + +1.2 840,907 + -0.0 840,907 + -0.2 840,907 + +0.3 840,907 + -3.7 840,907 + -0.0 840,907 + -0.1 840,908 - -1.8 840,908 - +1.2 840,908 - +0.9 840,922 - +1.6 840,922 - -1.3 840,935 - -1.2 840,946 + -2.0 840,947 - -2.6 840,947 - +0.2 841,177 + RR42_RS24555 0.17 +0.8 841,177 + RR42_RS24555 0.17 -0.8 841,177 + RR42_RS24555 0.17 +1.3 841,178 - RR42_RS24555 0.17 +0.6 841,337 + RR42_RS24555 0.28 -0.1 841,337 + RR42_RS24555 0.28 -0.5 841,338 - RR42_RS24555 0.28 +0.0 841,338 - RR42_RS24555 0.28 +0.7 841,338 - RR42_RS24555 0.28 +0.4 841,490 - RR42_RS24555 0.38 +0.4 841,862 + RR42_RS24555 0.63 +0.5 841,862 + RR42_RS24555 0.63 +1.2 841,863 - RR42_RS24555 0.63 -0.8 842,130 + RR42_RS24555 0.81 -0.5 842,192 + RR42_RS24555 0.85 -0.5 842,192 + RR42_RS24555 0.85 -0.3 842,213 + RR42_RS24555 0.87 -1.6 842,213 + RR42_RS24555 0.87 +0.0 842,261 + +0.7 842,372 + -1.0 842,373 - -0.2 842,388 - +0.7 842,388 - +2.0 842,388 - +0.0 842,466 - -1.3 842,818 + RR42_RS24560 0.32 -0.2 842,818 + RR42_RS24560 0.32 -0.3 842,818 + RR42_RS24560 0.32 -0.1 842,818 + RR42_RS24560 0.32 -0.0 842,818 + RR42_RS24560 0.32 +1.5 842,819 - RR42_RS24560 0.32 -0.3 842,819 - RR42_RS24560 0.32 +0.1 842,902 - RR42_RS24560 0.39 -1.3 842,902 - RR42_RS24560 0.39 +0.4 842,960 - RR42_RS24560 0.44 -1.4 843,062 - RR42_RS24560 0.53 -1.0 843,062 - RR42_RS24560 0.53 -1.5 843,062 - RR42_RS24560 0.53 -0.3 843,125 + RR42_RS24560 0.58 -0.7 843,141 - RR42_RS24560 0.60 +0.2 843,257 - RR42_RS24560 0.70 +0.1
Or see this region's nucleotide sequence