Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23170 and RR42_RS23175 are separated by 192 nucleotides RR42_RS23175 and RR42_RS23180 are separated by 219 nucleotides
RR42_RS23170: RR42_RS23170 - LysR family transcriptional regulator, at 530,650 to 531,585
_RS23170
RR42_RS23175: RR42_RS23175 - mandelate racemase, at 531,778 to 532,947
_RS23175
RR42_RS23180: RR42_RS23180 - ligand-gated channel, at 533,167 to 535,353
_RS23180
Position (kb)
531
532
533 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 530.837 kb on + strand, within RR42_RS23170 at 530.837 kb on + strand, within RR42_RS23170 at 530.837 kb on + strand, within RR42_RS23170 at 530.935 kb on + strand, within RR42_RS23170 at 530.935 kb on + strand, within RR42_RS23170 at 530.936 kb on - strand, within RR42_RS23170 at 530.936 kb on - strand, within RR42_RS23170 at 530.936 kb on - strand, within RR42_RS23170 at 530.936 kb on - strand, within RR42_RS23170 at 530.977 kb on + strand, within RR42_RS23170 at 530.977 kb on + strand, within RR42_RS23170 at 530.978 kb on - strand, within RR42_RS23170 at 530.978 kb on - strand, within RR42_RS23170 at 530.978 kb on - strand, within RR42_RS23170 at 531.068 kb on + strand, within RR42_RS23170 at 531.386 kb on + strand, within RR42_RS23170 at 531.386 kb on + strand, within RR42_RS23170 at 531.386 kb on + strand, within RR42_RS23170 at 531.386 kb on + strand, within RR42_RS23170 at 531.386 kb on + strand, within RR42_RS23170 at 531.386 kb on + strand, within RR42_RS23170 at 531.387 kb on - strand, within RR42_RS23170 at 531.387 kb on - strand, within RR42_RS23170 at 531.387 kb on - strand, within RR42_RS23170 at 531.387 kb on - strand, within RR42_RS23170 at 531.533 kb on + strand at 531.621 kb on + strand at 531.622 kb on - strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.630 kb on + strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.631 kb on - strand at 531.655 kb on - strand at 531.732 kb on - strand at 531.838 kb on + strand at 531.838 kb on + strand at 531.838 kb on + strand at 531.838 kb on + strand at 531.838 kb on + strand at 531.839 kb on - strand at 531.841 kb on - strand at 531.841 kb on - strand at 532.225 kb on + strand, within RR42_RS23175 at 532.240 kb on + strand, within RR42_RS23175 at 532.240 kb on + strand, within RR42_RS23175 at 532.244 kb on - strand, within RR42_RS23175 at 532.244 kb on - strand, within RR42_RS23175 at 532.244 kb on - strand, within RR42_RS23175 at 532.247 kb on - strand, within RR42_RS23175 at 532.291 kb on + strand, within RR42_RS23175 at 532.291 kb on + strand, within RR42_RS23175 at 532.292 kb on - strand, within RR42_RS23175 at 532.292 kb on - strand, within RR42_RS23175 at 532.308 kb on + strand, within RR42_RS23175 at 532.308 kb on + strand, within RR42_RS23175 at 532.309 kb on - strand, within RR42_RS23175 at 532.309 kb on - strand, within RR42_RS23175 at 532.309 kb on - strand, within RR42_RS23175 at 532.309 kb on - strand, within RR42_RS23175 at 532.486 kb on + strand, within RR42_RS23175 at 532.540 kb on + strand, within RR42_RS23175 at 532.543 kb on + strand, within RR42_RS23175 at 532.543 kb on + strand, within RR42_RS23175 at 532.543 kb on + strand, within RR42_RS23175 at 532.544 kb on - strand, within RR42_RS23175 at 532.610 kb on - strand, within RR42_RS23175 at 532.664 kb on - strand, within RR42_RS23175 at 532.678 kb on + strand, within RR42_RS23175 at 532.679 kb on - strand, within RR42_RS23175 at 532.679 kb on - strand, within RR42_RS23175 at 532.801 kb on + strand, within RR42_RS23175 at 532.802 kb on - strand, within RR42_RS23175 at 532.861 kb on + strand at 532.861 kb on + strand at 532.861 kb on + strand at 532.862 kb on - strand at 532.862 kb on - strand at 532.862 kb on - strand at 532.915 kb on + strand at 532.993 kb on + strand at 532.993 kb on + strand at 532.994 kb on - strand at 532.994 kb on - strand at 532.994 kb on - strand at 533.246 kb on + strand at 533.246 kb on + strand at 533.247 kb on - strand at 533.509 kb on + strand, within RR42_RS23180 at 533.510 kb on - strand, within RR42_RS23180 at 533.561 kb on - strand, within RR42_RS23180 at 533.561 kb on - strand, within RR42_RS23180 at 533.595 kb on + strand, within RR42_RS23180 at 533.669 kb on + strand, within RR42_RS23180 at 533.702 kb on - strand, within RR42_RS23180 at 533.702 kb on - strand, within RR42_RS23180 at 533.734 kb on + strand, within RR42_RS23180 at 533.734 kb on + strand, within RR42_RS23180 at 533.734 kb on + strand, within RR42_RS23180 at 533.734 kb on + strand, within RR42_RS23180 at 533.734 kb on + strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.735 kb on - strand, within RR42_RS23180 at 533.928 kb on - strand, within RR42_RS23180
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 530,837 + RR42_RS23170 0.20 +0.2 530,837 + RR42_RS23170 0.20 -0.1 530,837 + RR42_RS23170 0.20 +1.7 530,935 + RR42_RS23170 0.30 +0.2 530,935 + RR42_RS23170 0.30 +0.3 530,936 - RR42_RS23170 0.31 +0.7 530,936 - RR42_RS23170 0.31 +0.6 530,936 - RR42_RS23170 0.31 +0.2 530,936 - RR42_RS23170 0.31 +0.6 530,977 + RR42_RS23170 0.35 +0.4 530,977 + RR42_RS23170 0.35 -1.1 530,978 - RR42_RS23170 0.35 +0.5 530,978 - RR42_RS23170 0.35 +0.1 530,978 - RR42_RS23170 0.35 +0.7 531,068 + RR42_RS23170 0.45 +1.4 531,386 + RR42_RS23170 0.79 -0.9 531,386 + RR42_RS23170 0.79 +0.0 531,386 + RR42_RS23170 0.79 +0.7 531,386 + RR42_RS23170 0.79 +0.2 531,386 + RR42_RS23170 0.79 -0.0 531,386 + RR42_RS23170 0.79 -0.8 531,387 - RR42_RS23170 0.79 -0.6 531,387 - RR42_RS23170 0.79 +1.2 531,387 - RR42_RS23170 0.79 +0.1 531,387 - RR42_RS23170 0.79 +0.5 531,533 + +0.8 531,621 + +0.9 531,622 - +1.4 531,630 + +0.1 531,630 + +1.0 531,630 + -0.1 531,630 + +0.7 531,630 + -0.7 531,630 + +0.4 531,630 + +0.5 531,630 + -0.6 531,630 + +0.8 531,630 + +0.3 531,630 + +1.1 531,630 + +0.2 531,630 + +0.5 531,631 - +1.3 531,631 - -0.7 531,631 - -0.7 531,631 - +3.0 531,631 - +1.1 531,631 - -1.1 531,631 - -0.6 531,631 - -0.4 531,631 - -0.9 531,631 - +1.3 531,631 - -0.6 531,631 - +0.0 531,631 - -0.2 531,655 - -0.3 531,732 - +0.6 531,838 + -0.6 531,838 + +0.3 531,838 + +0.3 531,838 + +0.9 531,838 + -0.2 531,839 - +0.7 531,841 - +0.1 531,841 - +1.5 532,225 + RR42_RS23175 0.38 -0.6 532,240 + RR42_RS23175 0.39 -0.0 532,240 + RR42_RS23175 0.39 +0.5 532,244 - RR42_RS23175 0.40 +0.7 532,244 - RR42_RS23175 0.40 -0.9 532,244 - RR42_RS23175 0.40 -1.8 532,247 - RR42_RS23175 0.40 -0.6 532,291 + RR42_RS23175 0.44 -0.8 532,291 + RR42_RS23175 0.44 +1.1 532,292 - RR42_RS23175 0.44 -0.3 532,292 - RR42_RS23175 0.44 +0.5 532,308 + RR42_RS23175 0.45 +2.1 532,308 + RR42_RS23175 0.45 -0.5 532,309 - RR42_RS23175 0.45 +0.2 532,309 - RR42_RS23175 0.45 -0.5 532,309 - RR42_RS23175 0.45 +0.5 532,309 - RR42_RS23175 0.45 -0.5 532,486 + RR42_RS23175 0.61 +0.4 532,540 + RR42_RS23175 0.65 -1.5 532,543 + RR42_RS23175 0.65 +0.3 532,543 + RR42_RS23175 0.65 +0.1 532,543 + RR42_RS23175 0.65 -1.3 532,544 - RR42_RS23175 0.65 +2.3 532,610 - RR42_RS23175 0.71 -0.2 532,664 - RR42_RS23175 0.76 -0.5 532,678 + RR42_RS23175 0.77 -0.4 532,679 - RR42_RS23175 0.77 -0.5 532,679 - RR42_RS23175 0.77 +0.3 532,801 + RR42_RS23175 0.87 -0.9 532,802 - RR42_RS23175 0.88 -0.9 532,861 + +1.0 532,861 + -1.0 532,861 + +0.2 532,862 - +1.3 532,862 - -0.0 532,862 - -0.7 532,915 + -1.2 532,993 + +0.4 532,993 + -0.2 532,994 - -0.8 532,994 - -0.1 532,994 - -0.5 533,246 + +0.7 533,246 + +0.4 533,247 - +1.4 533,509 + RR42_RS23180 0.16 -2.2 533,510 - RR42_RS23180 0.16 +0.3 533,561 - RR42_RS23180 0.18 +0.0 533,561 - RR42_RS23180 0.18 -1.9 533,595 + RR42_RS23180 0.20 +0.4 533,669 + RR42_RS23180 0.23 +0.2 533,702 - RR42_RS23180 0.24 +0.1 533,702 - RR42_RS23180 0.24 -0.5 533,734 + RR42_RS23180 0.26 +0.3 533,734 + RR42_RS23180 0.26 +0.1 533,734 + RR42_RS23180 0.26 +0.4 533,734 + RR42_RS23180 0.26 -0.0 533,734 + RR42_RS23180 0.26 +0.5 533,735 - RR42_RS23180 0.26 +0.1 533,735 - RR42_RS23180 0.26 +0.1 533,735 - RR42_RS23180 0.26 +1.0 533,735 - RR42_RS23180 0.26 +0.1 533,735 - RR42_RS23180 0.26 +2.1 533,735 - RR42_RS23180 0.26 -0.1 533,735 - RR42_RS23180 0.26 +1.1 533,735 - RR42_RS23180 0.26 +0.1 533,735 - RR42_RS23180 0.26 +0.4 533,735 - RR42_RS23180 0.26 +0.6 533,928 - RR42_RS23180 0.35 -0.6
Or see this region's nucleotide sequence