Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22820 and RR42_RS22825 are separated by 127 nucleotides RR42_RS22825 and RR42_RS22830 are separated by 379 nucleotides
RR42_RS22820: RR42_RS22820 - lysine transporter LysE, at 452,642 to 453,271
_RS22820
RR42_RS22825: RR42_RS22825 - AsnC family transcriptional regulator, at 453,399 to 453,860
_RS22825
RR42_RS22830: RR42_RS22830 - phosphorylase, at 454,240 to 455,196
_RS22830
Position (kb) 453
454 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 452.466 kb on + strand at 452.547 kb on + strand at 452.548 kb on - strand at 452.553 kb on + strand at 452.553 kb on + strand at 452.643 kb on + strand at 452.896 kb on + strand, within RR42_RS22820 at 452.897 kb on - strand, within RR42_RS22820 at 452.937 kb on - strand, within RR42_RS22820 at 453.015 kb on + strand, within RR42_RS22820 at 453.015 kb on + strand, within RR42_RS22820 at 453.016 kb on - strand, within RR42_RS22820 at 453.025 kb on - strand, within RR42_RS22820 at 453.054 kb on + strand, within RR42_RS22820 at 453.055 kb on - strand, within RR42_RS22820 at 453.219 kb on + strand at 453.219 kb on + strand at 453.219 kb on + strand at 453.219 kb on + strand at 453.219 kb on + strand at 453.220 kb on - strand at 453.220 kb on - strand at 453.260 kb on + strand at 453.260 kb on + strand at 453.260 kb on + strand at 453.260 kb on + strand at 453.260 kb on + strand at 453.260 kb on + strand at 453.260 kb on + strand at 453.261 kb on - strand at 453.261 kb on - strand at 453.261 kb on - strand at 453.261 kb on - strand at 453.261 kb on - strand at 453.261 kb on - strand at 453.261 kb on - strand at 453.342 kb on - strand at 453.344 kb on + strand at 453.345 kb on - strand at 453.351 kb on - strand at 453.540 kb on + strand, within RR42_RS22825 at 453.540 kb on + strand, within RR42_RS22825 at 453.540 kb on + strand, within RR42_RS22825 at 453.543 kb on - strand, within RR42_RS22825 at 453.586 kb on + strand, within RR42_RS22825 at 453.692 kb on + strand, within RR42_RS22825 at 453.714 kb on + strand, within RR42_RS22825 at 453.714 kb on + strand, within RR42_RS22825 at 453.715 kb on - strand, within RR42_RS22825 at 453.751 kb on - strand, within RR42_RS22825 at 453.948 kb on + strand at 453.949 kb on - strand at 453.949 kb on - strand at 453.949 kb on - strand at 453.949 kb on - strand at 453.949 kb on - strand at 454.085 kb on + strand at 454.086 kb on - strand at 454.086 kb on - strand at 454.086 kb on - strand at 454.109 kb on + strand at 454.148 kb on + strand at 454.149 kb on - strand at 454.149 kb on - strand at 454.192 kb on + strand at 454.192 kb on + strand at 454.217 kb on + strand at 454.218 kb on - strand at 454.218 kb on - strand at 454.218 kb on - strand at 454.218 kb on - strand at 454.678 kb on + strand, within RR42_RS22830 at 454.678 kb on + strand, within RR42_RS22830 at 454.679 kb on - strand, within RR42_RS22830 at 454.679 kb on - strand, within RR42_RS22830 at 454.680 kb on + strand, within RR42_RS22830 at 454.681 kb on - strand, within RR42_RS22830
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 452,466 + -0.4 452,547 + -2.8 452,548 - +1.5 452,553 + +1.2 452,553 + +0.1 452,643 + +1.2 452,896 + RR42_RS22820 0.40 -1.0 452,897 - RR42_RS22820 0.40 +0.6 452,937 - RR42_RS22820 0.47 +0.2 453,015 + RR42_RS22820 0.59 +1.3 453,015 + RR42_RS22820 0.59 -0.4 453,016 - RR42_RS22820 0.59 -0.3 453,025 - RR42_RS22820 0.61 -0.8 453,054 + RR42_RS22820 0.65 -0.4 453,055 - RR42_RS22820 0.66 +0.3 453,219 + +0.4 453,219 + -0.3 453,219 + -1.2 453,219 + +0.9 453,219 + -0.6 453,220 - -0.3 453,220 - -0.9 453,260 + -0.4 453,260 + -1.1 453,260 + +1.0 453,260 + -0.1 453,260 + -0.9 453,260 + -1.6 453,260 + -0.0 453,261 - -0.2 453,261 - +0.8 453,261 - +0.6 453,261 - +0.9 453,261 - -0.8 453,261 - -1.8 453,261 - +0.2 453,342 - +1.0 453,344 + -0.2 453,345 - +1.0 453,351 - +0.9 453,540 + RR42_RS22825 0.31 -2.3 453,540 + RR42_RS22825 0.31 -0.4 453,540 + RR42_RS22825 0.31 -1.3 453,543 - RR42_RS22825 0.31 -0.1 453,586 + RR42_RS22825 0.40 -0.4 453,692 + RR42_RS22825 0.63 +0.4 453,714 + RR42_RS22825 0.68 -0.8 453,714 + RR42_RS22825 0.68 +0.1 453,715 - RR42_RS22825 0.68 -0.3 453,751 - RR42_RS22825 0.76 -0.0 453,948 + -1.3 453,949 - -0.3 453,949 - +0.5 453,949 - +0.2 453,949 - +1.5 453,949 - +0.5 454,085 + -2.1 454,086 - -0.2 454,086 - +0.4 454,086 - -0.2 454,109 + +1.6 454,148 + +1.6 454,149 - -0.3 454,149 - +0.6 454,192 + -2.8 454,192 + +0.9 454,217 + +0.0 454,218 - +1.3 454,218 - -0.8 454,218 - -0.0 454,218 - -0.3 454,678 + RR42_RS22830 0.46 -0.4 454,678 + RR42_RS22830 0.46 +0.3 454,679 - RR42_RS22830 0.46 +1.8 454,679 - RR42_RS22830 0.46 -0.6 454,680 + RR42_RS22830 0.46 +0.8 454,681 - RR42_RS22830 0.46 -0.4
Or see this region's nucleotide sequence