Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22800 and RR42_RS22805 are separated by 248 nucleotides RR42_RS22805 and RR42_RS22810 are separated by 38 nucleotides
RR42_RS22800: RR42_RS22800 - DSBA oxidoreductase, at 447,473 to 449,029
_RS22800
RR42_RS22805: RR42_RS22805 - LysR family transcriptional regulator, at 449,278 to 450,153
_RS22805
RR42_RS22810: RR42_RS22810 - 3-oxoacyl-ACP reductase, at 450,192 to 450,992
_RS22810
Position (kb) 449
450
451 Strain fitness (log2 ratio) -2
-1
0
1
2 at 448.357 kb on + strand, within RR42_RS22800 at 448.357 kb on + strand, within RR42_RS22800 at 448.357 kb on + strand, within RR42_RS22800 at 448.358 kb on - strand, within RR42_RS22800 at 448.447 kb on + strand, within RR42_RS22800 at 448.447 kb on + strand, within RR42_RS22800 at 448.448 kb on - strand, within RR42_RS22800 at 448.481 kb on - strand, within RR42_RS22800 at 448.875 kb on + strand at 449.243 kb on - strand at 449.373 kb on + strand, within RR42_RS22805 at 449.566 kb on + strand, within RR42_RS22805 at 449.713 kb on + strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.714 kb on - strand, within RR42_RS22805 at 449.715 kb on + strand, within RR42_RS22805 at 449.715 kb on + strand, within RR42_RS22805 at 450.035 kb on - strand, within RR42_RS22805 at 450.067 kb on + strand at 450.068 kb on - strand at 450.068 kb on - strand at 450.119 kb on - strand at 450.121 kb on - strand at 450.213 kb on - strand at 450.245 kb on + strand at 450.245 kb on + strand at 450.245 kb on + strand at 450.248 kb on - strand at 450.248 kb on - strand at 450.460 kb on + strand, within RR42_RS22810 at 450.461 kb on - strand, within RR42_RS22810 at 450.560 kb on + strand, within RR42_RS22810 at 450.625 kb on + strand, within RR42_RS22810 at 450.626 kb on - strand, within RR42_RS22810 at 450.634 kb on + strand, within RR42_RS22810 at 450.635 kb on - strand, within RR42_RS22810 at 450.635 kb on - strand, within RR42_RS22810 at 450.821 kb on - strand, within RR42_RS22810 at 450.892 kb on - strand, within RR42_RS22810 at 451.112 kb on + strand at 451.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 448,357 + RR42_RS22800 0.57 +0.6 448,357 + RR42_RS22800 0.57 -0.7 448,357 + RR42_RS22800 0.57 -0.1 448,358 - RR42_RS22800 0.57 +0.6 448,447 + RR42_RS22800 0.63 +0.3 448,447 + RR42_RS22800 0.63 +0.4 448,448 - RR42_RS22800 0.63 -0.3 448,481 - RR42_RS22800 0.65 -1.3 448,875 + +0.6 449,243 - +0.6 449,373 + RR42_RS22805 0.11 +0.2 449,566 + RR42_RS22805 0.33 -0.2 449,713 + RR42_RS22805 0.50 +0.6 449,714 - RR42_RS22805 0.50 -0.1 449,714 - RR42_RS22805 0.50 +0.5 449,714 - RR42_RS22805 0.50 +0.3 449,714 - RR42_RS22805 0.50 -1.1 449,715 + RR42_RS22805 0.50 +0.4 449,715 + RR42_RS22805 0.50 +0.4 450,035 - RR42_RS22805 0.86 +0.5 450,067 + +0.5 450,068 - +0.1 450,068 - -0.9 450,119 - -0.7 450,121 - -0.9 450,213 - -0.8 450,245 + -0.5 450,245 + +0.7 450,245 + +0.5 450,248 - +2.4 450,248 - -0.0 450,460 + RR42_RS22810 0.33 -1.6 450,461 - RR42_RS22810 0.34 -2.7 450,560 + RR42_RS22810 0.46 -0.8 450,625 + RR42_RS22810 0.54 -2.2 450,626 - RR42_RS22810 0.54 +1.2 450,634 + RR42_RS22810 0.55 -0.0 450,635 - RR42_RS22810 0.55 +0.0 450,635 - RR42_RS22810 0.55 -0.2 450,821 - RR42_RS22810 0.79 +0.3 450,892 - RR42_RS22810 0.87 -0.0 451,112 + +1.1 451,112 + +0.6
Or see this region's nucleotide sequence