Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22730 and RR42_RS22735 are separated by 20 nucleotides RR42_RS22735 and RR42_RS22740 are separated by 121 nucleotides RR42_RS22740 and RR42_RS22745 are separated by 294 nucleotides
RR42_RS22730: RR42_RS22730 - pyridine nucleotide-disulfide oxidoreductase, at 433,813 to 435,018
_RS22730
RR42_RS22735: RR42_RS22735 - transcriptional regulator, at 435,039 to 435,578
_RS22735
RR42_RS22740: RR42_RS22740 - oxidoreductase, at 435,700 to 436,494
_RS22740
RR42_RS22745: RR42_RS22745 - phosphoribosylglycinamide formyltransferase, at 436,789 to 437,949
_RS22745
Position (kb) 435
436
437 Strain fitness (log2 ratio) -2
-1
0
1
2 at 434.719 kb on + strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.720 kb on - strand, within RR42_RS22730 at 434.720 kb on - strand, within RR42_RS22730 at 435.011 kb on + strand at 435.016 kb on + strand at 435.105 kb on + strand, within RR42_RS22735 at 435.105 kb on + strand, within RR42_RS22735 at 435.106 kb on - strand, within RR42_RS22735 at 435.106 kb on - strand, within RR42_RS22735 at 435.452 kb on - strand, within RR42_RS22735 at 435.495 kb on - strand, within RR42_RS22735 at 435.605 kb on - strand at 435.615 kb on - strand at 435.617 kb on - strand at 435.712 kb on - strand at 435.780 kb on - strand, within RR42_RS22740 at 435.780 kb on - strand, within RR42_RS22740 at 436.034 kb on + strand, within RR42_RS22740 at 436.035 kb on - strand, within RR42_RS22740 at 436.035 kb on - strand, within RR42_RS22740 at 436.057 kb on - strand, within RR42_RS22740 at 436.101 kb on + strand, within RR42_RS22740 at 436.124 kb on + strand, within RR42_RS22740 at 436.124 kb on + strand, within RR42_RS22740 at 436.124 kb on + strand, within RR42_RS22740 at 436.125 kb on - strand, within RR42_RS22740 at 436.234 kb on + strand, within RR42_RS22740 at 436.537 kb on + strand at 436.537 kb on + strand at 436.538 kb on - strand at 436.538 kb on - strand at 436.635 kb on + strand at 436.635 kb on + strand at 436.635 kb on + strand at 436.636 kb on - strand at 436.707 kb on + strand at 436.707 kb on + strand at 436.708 kb on - strand at 436.708 kb on - strand at 436.722 kb on + strand at 436.723 kb on - strand at 437.140 kb on + strand, within RR42_RS22745 at 437.287 kb on + strand, within RR42_RS22745 at 437.288 kb on - strand, within RR42_RS22745 at 437.353 kb on + strand, within RR42_RS22745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 434,719 + RR42_RS22730 0.75 +1.2 434,719 + RR42_RS22730 0.75 -0.6 434,719 + RR42_RS22730 0.75 +0.5 434,720 - RR42_RS22730 0.75 +0.8 434,720 - RR42_RS22730 0.75 +0.2 435,011 + -0.7 435,016 + +0.8 435,105 + RR42_RS22735 0.12 -2.2 435,105 + RR42_RS22735 0.12 +0.1 435,106 - RR42_RS22735 0.12 -0.9 435,106 - RR42_RS22735 0.12 -0.1 435,452 - RR42_RS22735 0.76 +0.0 435,495 - RR42_RS22735 0.84 -0.7 435,605 - +1.1 435,615 - -0.7 435,617 - -2.0 435,712 - +0.2 435,780 - RR42_RS22740 0.10 +0.3 435,780 - RR42_RS22740 0.10 +0.7 436,034 + RR42_RS22740 0.42 -0.4 436,035 - RR42_RS22740 0.42 +0.4 436,035 - RR42_RS22740 0.42 -1.4 436,057 - RR42_RS22740 0.45 +0.1 436,101 + RR42_RS22740 0.50 +0.3 436,124 + RR42_RS22740 0.53 +0.4 436,124 + RR42_RS22740 0.53 +0.3 436,124 + RR42_RS22740 0.53 +2.5 436,125 - RR42_RS22740 0.53 +0.7 436,234 + RR42_RS22740 0.67 +0.5 436,537 + +0.4 436,537 + -0.1 436,538 - -0.4 436,538 - -0.6 436,635 + +0.7 436,635 + -1.6 436,635 + -0.3 436,636 - +0.2 436,707 + -0.3 436,707 + +1.3 436,708 - -0.2 436,708 - +1.3 436,722 + -1.0 436,723 - +1.8 437,140 + RR42_RS22745 0.30 -0.6 437,287 + RR42_RS22745 0.43 +0.6 437,288 - RR42_RS22745 0.43 +0.3 437,353 + RR42_RS22745 0.49 +0.6
Or see this region's nucleotide sequence