Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22455 and RR42_RS22460 are separated by 146 nucleotides RR42_RS22460 and RR42_RS22465 are separated by 67 nucleotides
RR42_RS22455: RR42_RS22455 - 2-aminoethylphosphonate aminotransferase, at 373,963 to 375,027
_RS22455
RR42_RS22460: RR42_RS22460 - ABC transporter, at 375,174 to 375,872
_RS22460
RR42_RS22465: RR42_RS22465 - membrane protein, at 375,940 to 377,994
_RS22465
Position (kb) 375
376 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 374.257 kb on + strand, within RR42_RS22455 at 374.438 kb on + strand, within RR42_RS22455 at 374.438 kb on + strand, within RR42_RS22455 at 374.438 kb on + strand, within RR42_RS22455 at 374.439 kb on - strand, within RR42_RS22455 at 374.439 kb on - strand, within RR42_RS22455 at 374.439 kb on - strand, within RR42_RS22455 at 374.605 kb on + strand, within RR42_RS22455 at 374.605 kb on + strand, within RR42_RS22455 at 374.606 kb on - strand, within RR42_RS22455 at 374.608 kb on + strand, within RR42_RS22455 at 374.608 kb on + strand, within RR42_RS22455 at 374.652 kb on + strand, within RR42_RS22455 at 374.652 kb on + strand, within RR42_RS22455 at 374.661 kb on + strand, within RR42_RS22455 at 374.672 kb on + strand, within RR42_RS22455 at 374.672 kb on + strand, within RR42_RS22455 at 374.672 kb on + strand, within RR42_RS22455 at 374.672 kb on + strand, within RR42_RS22455 at 374.672 kb on + strand, within RR42_RS22455 at 374.672 kb on + strand, within RR42_RS22455 at 374.673 kb on - strand, within RR42_RS22455 at 374.680 kb on + strand, within RR42_RS22455 at 374.680 kb on + strand, within RR42_RS22455 at 374.681 kb on - strand, within RR42_RS22455 at 374.681 kb on - strand, within RR42_RS22455 at 374.681 kb on - strand, within RR42_RS22455 at 374.683 kb on + strand, within RR42_RS22455 at 374.870 kb on - strand, within RR42_RS22455 at 374.914 kb on + strand, within RR42_RS22455 at 374.915 kb on - strand, within RR42_RS22455 at 374.915 kb on - strand, within RR42_RS22455 at 374.915 kb on - strand, within RR42_RS22455 at 374.915 kb on - strand, within RR42_RS22455 at 374.933 kb on + strand at 374.970 kb on + strand at 375.026 kb on - strand at 375.050 kb on + strand at 375.101 kb on + strand at 375.101 kb on + strand at 375.102 kb on - strand at 375.102 kb on - strand at 375.102 kb on - strand at 375.102 kb on - strand at 375.102 kb on - strand at 375.107 kb on + strand at 375.107 kb on + strand at 375.108 kb on - strand at 375.108 kb on - strand at 375.260 kb on + strand, within RR42_RS22460 at 375.471 kb on + strand, within RR42_RS22460 at 375.472 kb on - strand, within RR42_RS22460 at 375.664 kb on + strand, within RR42_RS22460 at 375.664 kb on + strand, within RR42_RS22460 at 375.664 kb on + strand, within RR42_RS22460 at 375.665 kb on - strand, within RR42_RS22460 at 375.665 kb on - strand, within RR42_RS22460 at 375.761 kb on + strand, within RR42_RS22460 at 375.761 kb on + strand, within RR42_RS22460 at 375.762 kb on - strand, within RR42_RS22460 at 375.847 kb on - strand at 375.847 kb on - strand at 375.860 kb on + strand at 375.861 kb on - strand at 375.875 kb on + strand at 375.887 kb on + strand at 375.887 kb on + strand at 375.887 kb on + strand at 375.887 kb on + strand at 375.888 kb on - strand at 375.888 kb on - strand at 375.925 kb on + strand at 375.925 kb on + strand at 375.933 kb on + strand at 376.065 kb on + strand at 376.193 kb on + strand, within RR42_RS22465 at 376.194 kb on - strand, within RR42_RS22465 at 376.634 kb on + strand, within RR42_RS22465 at 376.635 kb on - strand, within RR42_RS22465 at 376.766 kb on + strand, within RR42_RS22465 at 376.766 kb on + strand, within RR42_RS22465 at 376.767 kb on - strand, within RR42_RS22465 at 376.823 kb on + strand, within RR42_RS22465 at 376.824 kb on - strand, within RR42_RS22465
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 374,257 + RR42_RS22455 0.28 -0.6 374,438 + RR42_RS22455 0.45 -2.6 374,438 + RR42_RS22455 0.45 -1.4 374,438 + RR42_RS22455 0.45 -1.1 374,439 - RR42_RS22455 0.45 +0.2 374,439 - RR42_RS22455 0.45 -1.5 374,439 - RR42_RS22455 0.45 -2.5 374,605 + RR42_RS22455 0.60 -2.1 374,605 + RR42_RS22455 0.60 -4.2 374,606 - RR42_RS22455 0.60 -1.4 374,608 + RR42_RS22455 0.61 -2.8 374,608 + RR42_RS22455 0.61 -1.3 374,652 + RR42_RS22455 0.65 -0.3 374,652 + RR42_RS22455 0.65 -3.6 374,661 + RR42_RS22455 0.66 -2.0 374,672 + RR42_RS22455 0.67 -1.4 374,672 + RR42_RS22455 0.67 -1.9 374,672 + RR42_RS22455 0.67 -1.6 374,672 + RR42_RS22455 0.67 -1.7 374,672 + RR42_RS22455 0.67 -1.4 374,672 + RR42_RS22455 0.67 -3.2 374,673 - RR42_RS22455 0.67 -2.6 374,680 + RR42_RS22455 0.67 -3.0 374,680 + RR42_RS22455 0.67 -0.8 374,681 - RR42_RS22455 0.67 -3.0 374,681 - RR42_RS22455 0.67 -3.3 374,681 - RR42_RS22455 0.67 -1.6 374,683 + RR42_RS22455 0.68 -1.4 374,870 - RR42_RS22455 0.85 -3.1 374,914 + RR42_RS22455 0.89 -1.7 374,915 - RR42_RS22455 0.89 -0.5 374,915 - RR42_RS22455 0.89 +1.2 374,915 - RR42_RS22455 0.89 -0.0 374,915 - RR42_RS22455 0.89 -2.4 374,933 + -1.6 374,970 + -1.1 375,026 - +1.7 375,050 + +0.7 375,101 + -1.9 375,101 + -0.4 375,102 - -0.4 375,102 - -1.8 375,102 - -0.4 375,102 - +0.5 375,102 - -1.6 375,107 + +0.5 375,107 + -0.4 375,108 - -0.7 375,108 - +0.0 375,260 + RR42_RS22460 0.12 +0.3 375,471 + RR42_RS22460 0.42 +0.8 375,472 - RR42_RS22460 0.43 +0.8 375,664 + RR42_RS22460 0.70 +0.0 375,664 + RR42_RS22460 0.70 -0.0 375,664 + RR42_RS22460 0.70 +1.6 375,665 - RR42_RS22460 0.70 +0.7 375,665 - RR42_RS22460 0.70 +0.6 375,761 + RR42_RS22460 0.84 -1.5 375,761 + RR42_RS22460 0.84 +0.0 375,762 - RR42_RS22460 0.84 +0.6 375,847 - +0.4 375,847 - -0.0 375,860 + +1.2 375,861 - +0.7 375,875 + +0.1 375,887 + -1.1 375,887 + -0.3 375,887 + -1.1 375,887 + -1.4 375,888 - +0.5 375,888 - +1.0 375,925 + +0.6 375,925 + +0.4 375,933 + +0.6 376,065 + -2.3 376,193 + RR42_RS22465 0.12 +0.1 376,194 - RR42_RS22465 0.12 +0.2 376,634 + RR42_RS22465 0.34 +0.6 376,635 - RR42_RS22465 0.34 +0.9 376,766 + RR42_RS22465 0.40 +0.6 376,766 + RR42_RS22465 0.40 -0.4 376,767 - RR42_RS22465 0.40 +0.9 376,823 + RR42_RS22465 0.43 +0.7 376,824 - RR42_RS22465 0.43 -0.6
Or see this region's nucleotide sequence