Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22435 and RR42_RS22440 are separated by 32 nucleotides RR42_RS22440 and RR42_RS22445 overlap by 4 nucleotides RR42_RS22445 and RR42_RS22450 overlap by 4 nucleotides
RR42_RS22435: RR42_RS22435 - membrane protein, at 369,277 to 370,284
_RS22435
RR42_RS22440: RR42_RS22440 - ADP-glucose pyrophosphorylase, at 370,317 to 371,081
_RS22440
RR42_RS22445: RR42_RS22445 - phosphoenolpyruvate phosphomutase, at 371,078 to 372,784
_RS22445
RR42_RS22450: RR42_RS22450 - phosphonopyruvate decarboxylase, at 372,781 to 373,962
_RS22450
Position (kb) 371
372
373 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 370.216 kb on + strand at 370.216 kb on + strand at 370.220 kb on + strand at 370.346 kb on - strand at 370.650 kb on + strand, within RR42_RS22440 at 370.651 kb on - strand, within RR42_RS22440 at 370.651 kb on - strand, within RR42_RS22440 at 370.806 kb on + strand, within RR42_RS22440 at 370.807 kb on - strand, within RR42_RS22440 at 370.887 kb on + strand, within RR42_RS22440 at 370.887 kb on + strand, within RR42_RS22440 at 370.888 kb on - strand, within RR42_RS22440 at 370.960 kb on + strand, within RR42_RS22440 at 370.960 kb on + strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440 at 371.302 kb on + strand, within RR42_RS22445 at 371.750 kb on + strand, within RR42_RS22445 at 371.750 kb on + strand, within RR42_RS22445 at 371.750 kb on + strand, within RR42_RS22445 at 371.750 kb on + strand, within RR42_RS22445 at 371.750 kb on + strand, within RR42_RS22445 at 371.750 kb on + strand, within RR42_RS22445 at 371.751 kb on - strand, within RR42_RS22445 at 371.753 kb on + strand, within RR42_RS22445 at 371.753 kb on + strand, within RR42_RS22445 at 371.753 kb on + strand, within RR42_RS22445 at 371.753 kb on + strand, within RR42_RS22445 at 371.753 kb on + strand, within RR42_RS22445 at 371.754 kb on - strand, within RR42_RS22445 at 371.754 kb on - strand, within RR42_RS22445 at 371.754 kb on - strand, within RR42_RS22445 at 371.754 kb on - strand, within RR42_RS22445 at 371.946 kb on - strand, within RR42_RS22445 at 371.946 kb on - strand, within RR42_RS22445 at 371.966 kb on + strand, within RR42_RS22445 at 371.966 kb on + strand, within RR42_RS22445 at 371.966 kb on + strand, within RR42_RS22445 at 372.302 kb on + strand, within RR42_RS22445 at 372.302 kb on + strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.303 kb on - strand, within RR42_RS22445 at 372.304 kb on + strand, within RR42_RS22445 at 372.304 kb on + strand, within RR42_RS22445 at 372.305 kb on - strand, within RR42_RS22445 at 372.345 kb on + strand, within RR42_RS22445 at 372.401 kb on + strand, within RR42_RS22445 at 372.665 kb on + strand at 372.666 kb on - strand at 372.666 kb on - strand at 372.666 kb on - strand at 372.733 kb on + strand at 372.877 kb on + strand at 372.878 kb on - strand at 372.878 kb on - strand at 372.904 kb on + strand, within RR42_RS22450 at 372.904 kb on + strand, within RR42_RS22450 at 372.905 kb on - strand, within RR42_RS22450 at 372.905 kb on - strand, within RR42_RS22450 at 373.233 kb on + strand, within RR42_RS22450 at 373.233 kb on + strand, within RR42_RS22450 at 373.234 kb on - strand, within RR42_RS22450 at 373.234 kb on - strand, within RR42_RS22450 at 373.256 kb on - strand, within RR42_RS22450 at 373.416 kb on + strand, within RR42_RS22450 at 373.504 kb on + strand, within RR42_RS22450 at 373.504 kb on + strand, within RR42_RS22450 at 373.505 kb on - strand, within RR42_RS22450 at 373.505 kb on - strand, within RR42_RS22450 at 373.642 kb on + strand, within RR42_RS22450 at 373.642 kb on + strand, within RR42_RS22450
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 370,216 + -1.7 370,216 + -1.0 370,220 + -0.7 370,346 - -1.5 370,650 + RR42_RS22440 0.44 -1.1 370,651 - RR42_RS22440 0.44 -1.6 370,651 - RR42_RS22440 0.44 -1.4 370,806 + RR42_RS22440 0.64 -1.1 370,807 - RR42_RS22440 0.64 +1.2 370,887 + RR42_RS22440 0.75 +1.0 370,887 + RR42_RS22440 0.75 -2.3 370,888 - RR42_RS22440 0.75 -0.9 370,960 + RR42_RS22440 0.84 -3.2 370,960 + RR42_RS22440 0.84 -0.7 370,961 - RR42_RS22440 0.84 -1.8 370,961 - RR42_RS22440 0.84 -0.8 370,961 - RR42_RS22440 0.84 -1.9 370,961 - RR42_RS22440 0.84 -2.1 370,961 - RR42_RS22440 0.84 -0.4 371,302 + RR42_RS22445 0.13 -1.4 371,750 + RR42_RS22445 0.39 -1.9 371,750 + RR42_RS22445 0.39 -1.0 371,750 + RR42_RS22445 0.39 -0.0 371,750 + RR42_RS22445 0.39 -1.7 371,750 + RR42_RS22445 0.39 -2.6 371,750 + RR42_RS22445 0.39 -1.6 371,751 - RR42_RS22445 0.39 -0.7 371,753 + RR42_RS22445 0.40 -1.9 371,753 + RR42_RS22445 0.40 -2.7 371,753 + RR42_RS22445 0.40 -3.4 371,753 + RR42_RS22445 0.40 -2.3 371,753 + RR42_RS22445 0.40 -2.1 371,754 - RR42_RS22445 0.40 -1.2 371,754 - RR42_RS22445 0.40 -2.5 371,754 - RR42_RS22445 0.40 -1.2 371,754 - RR42_RS22445 0.40 -1.2 371,946 - RR42_RS22445 0.51 -1.7 371,946 - RR42_RS22445 0.51 -2.2 371,966 + RR42_RS22445 0.52 -2.6 371,966 + RR42_RS22445 0.52 -1.2 371,966 + RR42_RS22445 0.52 -0.5 372,302 + RR42_RS22445 0.72 -3.8 372,302 + RR42_RS22445 0.72 -1.1 372,303 - RR42_RS22445 0.72 +0.5 372,303 - RR42_RS22445 0.72 -2.4 372,303 - RR42_RS22445 0.72 -2.5 372,303 - RR42_RS22445 0.72 -2.5 372,303 - RR42_RS22445 0.72 -2.2 372,303 - RR42_RS22445 0.72 -1.1 372,303 - RR42_RS22445 0.72 +0.1 372,303 - RR42_RS22445 0.72 -1.5 372,303 - RR42_RS22445 0.72 -1.9 372,303 - RR42_RS22445 0.72 -2.1 372,304 + RR42_RS22445 0.72 -2.6 372,304 + RR42_RS22445 0.72 -2.1 372,305 - RR42_RS22445 0.72 -0.2 372,345 + RR42_RS22445 0.74 -0.2 372,401 + RR42_RS22445 0.78 -0.7 372,665 + -0.4 372,666 - -1.5 372,666 - -2.0 372,666 - -2.0 372,733 + -3.1 372,877 + -0.8 372,878 - -0.4 372,878 - -1.0 372,904 + RR42_RS22450 0.10 -1.7 372,904 + RR42_RS22450 0.10 -1.7 372,905 - RR42_RS22450 0.10 +0.5 372,905 - RR42_RS22450 0.10 -2.0 373,233 + RR42_RS22450 0.38 -2.6 373,233 + RR42_RS22450 0.38 -0.4 373,234 - RR42_RS22450 0.38 -1.9 373,234 - RR42_RS22450 0.38 -0.8 373,256 - RR42_RS22450 0.40 -3.8 373,416 + RR42_RS22450 0.54 -1.2 373,504 + RR42_RS22450 0.61 -0.8 373,504 + RR42_RS22450 0.61 -3.6 373,505 - RR42_RS22450 0.61 -2.3 373,505 - RR42_RS22450 0.61 -1.5 373,642 + RR42_RS22450 0.73 -3.6 373,642 + RR42_RS22450 0.73 -2.0
Or see this region's nucleotide sequence