Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22420 and RR42_RS22425 are separated by 312 nucleotides RR42_RS22425 and RR42_RS22430 are separated by 8 nucleotides RR42_RS22430 and RR42_RS22435 overlap by 4 nucleotides
RR42_RS22420: RR42_RS22420 - penicillin-binding protein, at 365,025 to 367,448
_RS22420
RR42_RS22425: RR42_RS22425 - CDP-alcohol phosphatidyltransferase, at 367,761 to 368,429
_RS22425
RR42_RS22430: RR42_RS22430 - 2OG-Fe(II) oxygenase, at 368,438 to 369,280
_RS22430
RR42_RS22435: RR42_RS22435 - membrane protein, at 369,277 to 370,284
_RS22435
Position (kb) 367
368
369 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 366.997 kb on - strand, within RR42_RS22420 at 367.138 kb on + strand, within RR42_RS22420 at 367.618 kb on + strand at 367.618 kb on + strand at 367.619 kb on - strand at 367.619 kb on - strand at 367.640 kb on + strand at 367.712 kb on + strand at 367.713 kb on - strand at 367.715 kb on + strand at 367.715 kb on + strand at 367.716 kb on - strand at 367.716 kb on - strand at 367.809 kb on - strand at 367.809 kb on - strand at 367.953 kb on + strand, within RR42_RS22425 at 367.953 kb on + strand, within RR42_RS22425 at 368.038 kb on + strand, within RR42_RS22425 at 368.277 kb on + strand, within RR42_RS22425 at 368.277 kb on + strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.377 kb on - strand at 368.714 kb on + strand, within RR42_RS22430 at 368.715 kb on - strand, within RR42_RS22430 at 368.769 kb on + strand, within RR42_RS22430 at 368.793 kb on - strand, within RR42_RS22430 at 368.793 kb on - strand, within RR42_RS22430 at 368.876 kb on + strand, within RR42_RS22430 at 368.876 kb on + strand, within RR42_RS22430 at 368.876 kb on + strand, within RR42_RS22430 at 368.877 kb on - strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.886 kb on - strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.889 kb on - strand, within RR42_RS22430 at 368.889 kb on - strand, within RR42_RS22430 at 368.891 kb on + strand, within RR42_RS22430 at 368.891 kb on + strand, within RR42_RS22430 at 368.892 kb on - strand, within RR42_RS22430 at 368.936 kb on + strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.940 kb on - strand, within RR42_RS22430 at 369.002 kb on + strand, within RR42_RS22430 at 369.155 kb on + strand, within RR42_RS22430 at 369.156 kb on - strand, within RR42_RS22430 at 369.156 kb on - strand, within RR42_RS22430 at 369.202 kb on + strand at 369.221 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 366,997 - RR42_RS22420 0.81 +0.7 367,138 + RR42_RS22420 0.87 +0.3 367,618 + -0.2 367,618 + +1.2 367,619 - -1.8 367,619 - -3.3 367,640 + +0.3 367,712 + -0.1 367,713 - -1.1 367,715 + +0.2 367,715 + +0.1 367,716 - -2.2 367,716 - -2.3 367,809 - +0.7 367,809 - +0.0 367,953 + RR42_RS22425 0.29 -2.4 367,953 + RR42_RS22425 0.29 -2.5 368,038 + RR42_RS22425 0.41 -1.7 368,277 + RR42_RS22425 0.77 -1.1 368,277 + RR42_RS22425 0.77 -1.8 368,278 - RR42_RS22425 0.77 -2.7 368,278 - RR42_RS22425 0.77 -0.5 368,278 - RR42_RS22425 0.77 +0.6 368,377 - -0.5 368,714 + RR42_RS22430 0.33 -4.3 368,715 - RR42_RS22430 0.33 -1.7 368,769 + RR42_RS22430 0.39 -1.0 368,793 - RR42_RS22430 0.42 -0.5 368,793 - RR42_RS22430 0.42 +0.5 368,876 + RR42_RS22430 0.52 -1.0 368,876 + RR42_RS22430 0.52 +1.4 368,876 + RR42_RS22430 0.52 -0.3 368,877 - RR42_RS22430 0.52 -1.6 368,885 + RR42_RS22430 0.53 -1.0 368,885 + RR42_RS22430 0.53 +0.1 368,885 + RR42_RS22430 0.53 -0.7 368,885 + RR42_RS22430 0.53 -1.1 368,885 + RR42_RS22430 0.53 -0.9 368,885 + RR42_RS22430 0.53 -0.0 368,886 - RR42_RS22430 0.53 -1.0 368,888 + RR42_RS22430 0.53 +0.2 368,888 + RR42_RS22430 0.53 -1.4 368,888 + RR42_RS22430 0.53 -1.2 368,888 + RR42_RS22430 0.53 -2.4 368,889 - RR42_RS22430 0.53 +1.2 368,889 - RR42_RS22430 0.53 -1.7 368,891 + RR42_RS22430 0.54 -0.4 368,891 + RR42_RS22430 0.54 -0.6 368,892 - RR42_RS22430 0.54 -0.7 368,936 + RR42_RS22430 0.59 -1.4 368,937 - RR42_RS22430 0.59 -0.8 368,937 - RR42_RS22430 0.59 -2.3 368,937 - RR42_RS22430 0.59 +0.8 368,937 - RR42_RS22430 0.59 -1.7 368,940 - RR42_RS22430 0.60 -3.3 369,002 + RR42_RS22430 0.67 -1.1 369,155 + RR42_RS22430 0.85 -1.1 369,156 - RR42_RS22430 0.85 -3.3 369,156 - RR42_RS22430 0.85 -2.8 369,202 + -0.7 369,221 + -1.7
Or see this region's nucleotide sequence