Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS22420

Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS22415 and RR42_RS22420 are separated by 141 nucleotidesRR42_RS22420 and RR42_RS22425 are separated by 312 nucleotidesRR42_RS22425 and RR42_RS22430 are separated by 8 nucleotides RR42_RS22415: RR42_RS22415 - hypothetical protein, at 364,047 to 364,883 _RS22415 RR42_RS22420: RR42_RS22420 - penicillin-binding protein, at 365,025 to 367,448 _RS22420 RR42_RS22425: RR42_RS22425 - CDP-alcohol phosphatidyltransferase, at 367,761 to 368,429 _RS22425 RR42_RS22430: RR42_RS22430 - 2OG-Fe(II) oxygenase, at 368,438 to 369,280 _RS22430 Position (kb) 365 366 367 368Strain fitness (log2 ratio) -3 -2 -1 0 1at 364.269 kb on + strand, within RR42_RS22415at 364.270 kb on - strand, within RR42_RS22415at 364.587 kb on + strand, within RR42_RS22415at 364.588 kb on - strand, within RR42_RS22415at 364.593 kb on + strand, within RR42_RS22415at 364.593 kb on + strand, within RR42_RS22415at 364.593 kb on + strand, within RR42_RS22415at 364.620 kb on - strand, within RR42_RS22415at 364.707 kb on + strand, within RR42_RS22415at 364.708 kb on - strand, within RR42_RS22415at 364.708 kb on - strand, within RR42_RS22415at 364.737 kb on + strand, within RR42_RS22415at 364.737 kb on + strand, within RR42_RS22415at 364.762 kb on - strand, within RR42_RS22415at 364.779 kb on + strand, within RR42_RS22415at 364.821 kb on + strandat 364.822 kb on - strandat 364.833 kb on + strandat 364.834 kb on - strandat 365.139 kb on + strandat 365.139 kb on + strandat 365.139 kb on + strandat 365.139 kb on + strandat 365.286 kb on - strand, within RR42_RS22420at 365.388 kb on - strand, within RR42_RS22420at 365.529 kb on + strand, within RR42_RS22420at 365.529 kb on + strand, within RR42_RS22420at 365.556 kb on + strand, within RR42_RS22420at 365.557 kb on - strand, within RR42_RS22420at 365.557 kb on - strand, within RR42_RS22420at 365.557 kb on - strand, within RR42_RS22420at 365.580 kb on + strand, within RR42_RS22420at 365.581 kb on - strand, within RR42_RS22420at 365.616 kb on + strand, within RR42_RS22420at 365.616 kb on + strand, within RR42_RS22420at 365.616 kb on + strand, within RR42_RS22420at 365.691 kb on + strand, within RR42_RS22420at 365.692 kb on - strand, within RR42_RS22420at 365.692 kb on - strand, within RR42_RS22420at 365.694 kb on + strand, within RR42_RS22420at 365.694 kb on + strand, within RR42_RS22420at 365.694 kb on + strand, within RR42_RS22420at 365.694 kb on + strand, within RR42_RS22420at 365.695 kb on - strand, within RR42_RS22420at 365.695 kb on - strand, within RR42_RS22420at 365.695 kb on - strand, within RR42_RS22420at 365.763 kb on + strand, within RR42_RS22420at 365.788 kb on - strand, within RR42_RS22420at 365.788 kb on - strand, within RR42_RS22420at 365.788 kb on - strand, within RR42_RS22420at 365.914 kb on - strand, within RR42_RS22420at 365.914 kb on - strand, within RR42_RS22420at 365.914 kb on - strand, within RR42_RS22420at 365.914 kb on - strand, within RR42_RS22420at 365.923 kb on - strand, within RR42_RS22420at 365.923 kb on - strand, within RR42_RS22420at 366.259 kb on + strand, within RR42_RS22420at 366.259 kb on + strand, within RR42_RS22420at 366.495 kb on + strand, within RR42_RS22420at 366.657 kb on + strand, within RR42_RS22420at 366.997 kb on - strand, within RR42_RS22420at 367.138 kb on + strand, within RR42_RS22420at 367.618 kb on + strandat 367.618 kb on + strandat 367.619 kb on - strandat 367.619 kb on - strandat 367.640 kb on + strandat 367.712 kb on + strandat 367.713 kb on - strandat 367.715 kb on + strandat 367.715 kb on + strandat 367.716 kb on - strandat 367.716 kb on - strandat 367.809 kb on - strandat 367.809 kb on - strandat 367.953 kb on + strand, within RR42_RS22425at 367.953 kb on + strand, within RR42_RS22425at 368.038 kb on + strand, within RR42_RS22425at 368.277 kb on + strand, within RR42_RS22425at 368.277 kb on + strand, within RR42_RS22425at 368.278 kb on - strand, within RR42_RS22425at 368.278 kb on - strand, within RR42_RS22425at 368.278 kb on - strand, within RR42_RS22425at 368.377 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml
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364,269 + RR42_RS22415 0.27 -0.6
364,270 - RR42_RS22415 0.27 +0.7
364,587 + RR42_RS22415 0.65 +1.0
364,588 - RR42_RS22415 0.65 -0.5
364,593 + RR42_RS22415 0.65 +0.0
364,593 + RR42_RS22415 0.65 +0.3
364,593 + RR42_RS22415 0.65 -1.4
364,620 - RR42_RS22415 0.68 +0.1
364,707 + RR42_RS22415 0.79 -0.0
364,708 - RR42_RS22415 0.79 +0.1
364,708 - RR42_RS22415 0.79 +0.8
364,737 + RR42_RS22415 0.82 +1.1
364,737 + RR42_RS22415 0.82 -2.7
364,762 - RR42_RS22415 0.85 +1.5
364,779 + RR42_RS22415 0.87 -1.6
364,821 + +0.9
364,822 - -1.1
364,833 + +0.0
364,834 - -0.1
365,139 + +0.5
365,139 + +0.0
365,139 + -0.2
365,139 + -0.1
365,286 - RR42_RS22420 0.11 +0.9
365,388 - RR42_RS22420 0.15 +1.2
365,529 + RR42_RS22420 0.21 +0.6
365,529 + RR42_RS22420 0.21 +0.5
365,556 + RR42_RS22420 0.22 +0.8
365,557 - RR42_RS22420 0.22 +0.6
365,557 - RR42_RS22420 0.22 +0.5
365,557 - RR42_RS22420 0.22 +1.5
365,580 + RR42_RS22420 0.23 +0.2
365,581 - RR42_RS22420 0.23 +0.1
365,616 + RR42_RS22420 0.24 +0.2
365,616 + RR42_RS22420 0.24 -0.5
365,616 + RR42_RS22420 0.24 +0.5
365,691 + RR42_RS22420 0.27 +1.4
365,692 - RR42_RS22420 0.28 +0.8
365,692 - RR42_RS22420 0.28 +0.6
365,694 + RR42_RS22420 0.28 -2.1
365,694 + RR42_RS22420 0.28 +0.5
365,694 + RR42_RS22420 0.28 +0.4
365,694 + RR42_RS22420 0.28 +1.7
365,695 - RR42_RS22420 0.28 +1.6
365,695 - RR42_RS22420 0.28 +1.2
365,695 - RR42_RS22420 0.28 -0.3
365,763 + RR42_RS22420 0.30 +0.6
365,788 - RR42_RS22420 0.31 +0.4
365,788 - RR42_RS22420 0.31 -1.8
365,788 - RR42_RS22420 0.31 +0.6
365,914 - RR42_RS22420 0.37 -0.7
365,914 - RR42_RS22420 0.37 -0.0
365,914 - RR42_RS22420 0.37 +0.6
365,914 - RR42_RS22420 0.37 +1.2
365,923 - RR42_RS22420 0.37 +0.2
365,923 - RR42_RS22420 0.37 +0.3
366,259 + RR42_RS22420 0.51 +1.2
366,259 + RR42_RS22420 0.51 -0.8
366,495 + RR42_RS22420 0.61 -2.2
366,657 + RR42_RS22420 0.67 +0.3
366,997 - RR42_RS22420 0.81 +0.7
367,138 + RR42_RS22420 0.87 +0.3
367,618 + -0.2
367,618 + +1.2
367,619 - -1.8
367,619 - -3.3
367,640 + +0.3
367,712 + -0.1
367,713 - -1.1
367,715 + +0.2
367,715 + +0.1
367,716 - -2.2
367,716 - -2.3
367,809 - +0.7
367,809 - +0.0
367,953 + RR42_RS22425 0.29 -2.4
367,953 + RR42_RS22425 0.29 -2.5
368,038 + RR42_RS22425 0.41 -1.7
368,277 + RR42_RS22425 0.77 -1.1
368,277 + RR42_RS22425 0.77 -1.8
368,278 - RR42_RS22425 0.77 -2.7
368,278 - RR42_RS22425 0.77 -0.5
368,278 - RR42_RS22425 0.77 +0.6
368,377 - -0.5

Or see this region's nucleotide sequence