Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22270 and RR42_RS22275 are separated by 257 nucleotides RR42_RS22275 and RR42_RS22280 are separated by 108 nucleotides RR42_RS22280 and RR42_RS22285 are separated by 72 nucleotides
RR42_RS22270: RR42_RS22270 - hypothetical protein, at 337,336 to 339,390
_RS22270
RR42_RS22275: RR42_RS22275 - hypothetical protein, at 339,648 to 339,914
_RS22275
RR42_RS22280: RR42_RS22280 - cupin, at 340,023 to 340,478
_RS22280
RR42_RS22285: RR42_RS22285 - methionine sulfoxide reductase A, at 340,551 to 341,291
_RS22285
Position (kb) 339
340 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 338.719 kb on + strand, within RR42_RS22270 at 338.719 kb on + strand, within RR42_RS22270 at 338.719 kb on + strand, within RR42_RS22270 at 338.719 kb on + strand, within RR42_RS22270 at 338.720 kb on - strand, within RR42_RS22270 at 338.720 kb on - strand, within RR42_RS22270 at 338.720 kb on - strand, within RR42_RS22270 at 338.762 kb on + strand, within RR42_RS22270 at 339.367 kb on + strand at 339.367 kb on + strand at 339.368 kb on - strand at 339.377 kb on + strand at 339.377 kb on + strand at 339.377 kb on + strand at 339.649 kb on + strand at 339.656 kb on + strand at 339.656 kb on + strand at 339.661 kb on + strand at 339.661 kb on + strand at 339.661 kb on + strand at 339.662 kb on - strand at 339.662 kb on - strand at 339.662 kb on - strand at 339.662 kb on - strand at 339.763 kb on + strand, within RR42_RS22275 at 339.764 kb on - strand, within RR42_RS22275 at 339.825 kb on + strand, within RR42_RS22275 at 339.932 kb on - strand at 339.967 kb on + strand at 339.967 kb on + strand at 339.968 kb on - strand at 340.083 kb on + strand, within RR42_RS22280 at 340.083 kb on + strand, within RR42_RS22280 at 340.120 kb on + strand, within RR42_RS22280 at 340.120 kb on + strand, within RR42_RS22280 at 340.120 kb on + strand, within RR42_RS22280 at 340.120 kb on + strand, within RR42_RS22280 at 340.121 kb on - strand, within RR42_RS22280 at 340.121 kb on - strand, within RR42_RS22280 at 340.121 kb on - strand, within RR42_RS22280 at 340.194 kb on + strand, within RR42_RS22280 at 340.194 kb on + strand, within RR42_RS22280 at 340.194 kb on + strand, within RR42_RS22280 at 340.194 kb on + strand, within RR42_RS22280 at 340.195 kb on - strand, within RR42_RS22280 at 340.195 kb on - strand, within RR42_RS22280 at 340.437 kb on + strand at 340.651 kb on + strand, within RR42_RS22285 at 340.651 kb on + strand, within RR42_RS22285 at 340.652 kb on - strand, within RR42_RS22285 at 340.690 kb on + strand, within RR42_RS22285 at 340.691 kb on - strand, within RR42_RS22285 at 340.779 kb on - strand, within RR42_RS22285 at 340.780 kb on + strand, within RR42_RS22285 at 340.780 kb on + strand, within RR42_RS22285 at 340.781 kb on - strand, within RR42_RS22285 at 340.781 kb on - strand, within RR42_RS22285 at 340.781 kb on - strand, within RR42_RS22285 at 340.835 kb on - strand, within RR42_RS22285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 338,719 + RR42_RS22270 0.67 +1.2 338,719 + RR42_RS22270 0.67 +0.0 338,719 + RR42_RS22270 0.67 +1.2 338,719 + RR42_RS22270 0.67 +0.6 338,720 - RR42_RS22270 0.67 -1.6 338,720 - RR42_RS22270 0.67 -2.6 338,720 - RR42_RS22270 0.67 -2.1 338,762 + RR42_RS22270 0.69 +1.6 339,367 + -0.5 339,367 + +4.2 339,368 - -0.4 339,377 + -0.5 339,377 + -0.4 339,377 + -0.8 339,649 + +1.3 339,656 + +1.5 339,656 + -0.1 339,661 + +1.4 339,661 + -2.2 339,661 + +2.5 339,662 - -0.1 339,662 - -0.2 339,662 - +0.6 339,662 - +0.7 339,763 + RR42_RS22275 0.43 -1.8 339,764 - RR42_RS22275 0.43 -1.1 339,825 + RR42_RS22275 0.66 -0.2 339,932 - -0.3 339,967 + +0.4 339,967 + +1.6 339,968 - -1.4 340,083 + RR42_RS22280 0.13 +1.2 340,083 + RR42_RS22280 0.13 +0.9 340,120 + RR42_RS22280 0.21 +0.9 340,120 + RR42_RS22280 0.21 +0.9 340,120 + RR42_RS22280 0.21 +1.0 340,120 + RR42_RS22280 0.21 +0.2 340,121 - RR42_RS22280 0.21 -0.7 340,121 - RR42_RS22280 0.21 +0.8 340,121 - RR42_RS22280 0.21 +0.4 340,194 + RR42_RS22280 0.38 +0.6 340,194 + RR42_RS22280 0.38 -0.9 340,194 + RR42_RS22280 0.38 -0.4 340,194 + RR42_RS22280 0.38 +1.3 340,195 - RR42_RS22280 0.38 +0.5 340,195 - RR42_RS22280 0.38 +0.6 340,437 + -0.1 340,651 + RR42_RS22285 0.13 +0.3 340,651 + RR42_RS22285 0.13 -0.4 340,652 - RR42_RS22285 0.14 +0.4 340,690 + RR42_RS22285 0.19 -1.4 340,691 - RR42_RS22285 0.19 -0.4 340,779 - RR42_RS22285 0.31 -0.1 340,780 + RR42_RS22285 0.31 -0.8 340,780 + RR42_RS22285 0.31 -0.4 340,781 - RR42_RS22285 0.31 +0.2 340,781 - RR42_RS22285 0.31 -0.8 340,781 - RR42_RS22285 0.31 +0.1 340,835 - RR42_RS22285 0.38 +0.6
Or see this region's nucleotide sequence