Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22235 and RR42_RS22240 are separated by 821 nucleotides RR42_RS22240 and RR42_RS22245 are separated by 364 nucleotides
RR42_RS22235: RR42_RS22235 - peptidase M19, at 326,256 to 326,885
_RS22235
RR42_RS22240: RR42_RS22240 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 327,707 to 329,008
_RS22240
RR42_RS22245: RR42_RS22245 - cytochrome C biogenesis protein, at 329,373 to 330,320
_RS22245
Position (kb)
327
328
329
330 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 326.827 kb on + strand at 326.828 kb on - strand at 326.828 kb on - strand at 327.005 kb on - strand at 327.651 kb on + strand at 327.652 kb on - strand at 327.652 kb on - strand at 327.697 kb on - strand at 327.751 kb on + strand at 327.752 kb on - strand at 327.752 kb on - strand at 327.752 kb on - strand at 327.755 kb on - strand at 327.902 kb on - strand, within RR42_RS22240 at 327.902 kb on - strand, within RR42_RS22240 at 327.902 kb on - strand, within RR42_RS22240 at 328.196 kb on + strand, within RR42_RS22240 at 328.196 kb on + strand, within RR42_RS22240 at 328.197 kb on - strand, within RR42_RS22240 at 328.197 kb on - strand, within RR42_RS22240 at 328.724 kb on + strand, within RR42_RS22240 at 328.850 kb on + strand, within RR42_RS22240 at 329.007 kb on - strand at 329.024 kb on + strand at 329.176 kb on + strand at 329.177 kb on - strand at 329.177 kb on - strand at 329.177 kb on - strand at 329.177 kb on - strand at 329.197 kb on - strand at 329.228 kb on + strand at 329.228 kb on + strand at 329.295 kb on + strand at 329.295 kb on + strand at 329.296 kb on - strand at 329.297 kb on + strand at 329.297 kb on + strand at 329.297 kb on + strand at 329.297 kb on + strand at 329.298 kb on - strand at 329.298 kb on - strand at 329.301 kb on + strand at 329.302 kb on - strand at 329.302 kb on - strand at 329.302 kb on - strand at 329.379 kb on + strand at 329.380 kb on - strand at 329.391 kb on + strand at 329.392 kb on - strand at 329.466 kb on + strand at 329.467 kb on - strand at 329.467 kb on - strand at 329.529 kb on + strand, within RR42_RS22245 at 329.530 kb on - strand, within RR42_RS22245 at 329.587 kb on - strand, within RR42_RS22245 at 329.640 kb on + strand, within RR42_RS22245 at 329.640 kb on + strand, within RR42_RS22245 at 329.834 kb on + strand, within RR42_RS22245 at 329.834 kb on + strand, within RR42_RS22245 at 329.983 kb on - strand, within RR42_RS22245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 326,827 + +1.8 326,828 - +0.2 326,828 - -0.8 327,005 - -0.8 327,651 + -0.4 327,652 - -0.1 327,652 - -0.9 327,697 - +0.4 327,751 + -0.4 327,752 - -1.1 327,752 - -0.2 327,752 - +1.6 327,755 - +1.5 327,902 - RR42_RS22240 0.15 +1.4 327,902 - RR42_RS22240 0.15 -0.5 327,902 - RR42_RS22240 0.15 -0.7 328,196 + RR42_RS22240 0.38 -0.9 328,196 + RR42_RS22240 0.38 +0.2 328,197 - RR42_RS22240 0.38 -1.5 328,197 - RR42_RS22240 0.38 -0.4 328,724 + RR42_RS22240 0.78 -1.7 328,850 + RR42_RS22240 0.88 +0.9 329,007 - -1.6 329,024 + -0.4 329,176 + -1.4 329,177 - +1.8 329,177 - +1.0 329,177 - +1.3 329,177 - -0.3 329,197 - -0.8 329,228 + +0.1 329,228 + +1.2 329,295 + +0.5 329,295 + -0.4 329,296 - -0.5 329,297 + +0.2 329,297 + +1.0 329,297 + -0.7 329,297 + -1.1 329,298 - -0.3 329,298 - -0.7 329,301 + -1.8 329,302 - -2.2 329,302 - -0.3 329,302 - -1.8 329,379 + -1.4 329,380 - -0.2 329,391 + -1.2 329,392 - +0.0 329,466 + -0.4 329,467 - -1.4 329,467 - +0.5 329,529 + RR42_RS22245 0.16 +0.3 329,530 - RR42_RS22245 0.17 +0.2 329,587 - RR42_RS22245 0.23 -0.2 329,640 + RR42_RS22245 0.28 +0.2 329,640 + RR42_RS22245 0.28 -0.4 329,834 + RR42_RS22245 0.49 +0.1 329,834 + RR42_RS22245 0.49 +0.2 329,983 - RR42_RS22245 0.64 -0.5
Or see this region's nucleotide sequence