Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22110 and RR42_RS22115 are separated by 2 nucleotides RR42_RS22115 and RR42_RS22120 are separated by 59 nucleotides RR42_RS22120 and RR42_RS22125 are separated by 277 nucleotides
RR42_RS22110: RR42_RS22110 - type VI secretion protein ImpG, at 299,996 to 301,819
_RS22110
RR42_RS22115: RR42_RS22115 - type VI secretion protein, at 301,822 to 302,868
_RS22115
RR42_RS22120: RR42_RS22120 - type VI secretion protein, at 302,928 to 303,434
_RS22120
RR42_RS22125: RR42_RS22125 - hypothetical protein, at 303,712 to 305,112
_RS22125
Position (kb)
301
302
303 Strain fitness (log2 ratio)
-2
-1
0
1 at 301.014 kb on - strand, within RR42_RS22110 at 301.176 kb on - strand, within RR42_RS22110 at 301.345 kb on + strand, within RR42_RS22110 at 301.346 kb on - strand, within RR42_RS22110 at 301.473 kb on - strand, within RR42_RS22110 at 301.736 kb on + strand at 301.897 kb on + strand at 302.029 kb on + strand, within RR42_RS22115 at 302.029 kb on + strand, within RR42_RS22115 at 302.029 kb on + strand, within RR42_RS22115 at 302.029 kb on + strand, within RR42_RS22115 at 302.029 kb on + strand, within RR42_RS22115 at 302.030 kb on - strand, within RR42_RS22115 at 302.030 kb on - strand, within RR42_RS22115 at 302.030 kb on - strand, within RR42_RS22115 at 302.030 kb on - strand, within RR42_RS22115 at 302.030 kb on - strand, within RR42_RS22115 at 302.097 kb on - strand, within RR42_RS22115 at 302.098 kb on + strand, within RR42_RS22115 at 302.098 kb on + strand, within RR42_RS22115 at 302.125 kb on + strand, within RR42_RS22115 at 302.236 kb on + strand, within RR42_RS22115 at 302.287 kb on + strand, within RR42_RS22115 at 302.288 kb on - strand, within RR42_RS22115 at 302.288 kb on - strand, within RR42_RS22115 at 302.395 kb on - strand, within RR42_RS22115 at 302.465 kb on - strand, within RR42_RS22115 at 302.539 kb on + strand, within RR42_RS22115 at 302.558 kb on + strand, within RR42_RS22115 at 302.653 kb on + strand, within RR42_RS22115 at 302.654 kb on - strand, within RR42_RS22115 at 302.663 kb on - strand, within RR42_RS22115 at 302.749 kb on + strand, within RR42_RS22115 at 302.896 kb on + strand at 302.896 kb on + strand at 302.897 kb on - strand at 302.901 kb on + strand at 302.975 kb on + strand at 302.975 kb on + strand at 302.976 kb on - strand at 302.978 kb on - strand at 303.095 kb on + strand, within RR42_RS22120 at 303.138 kb on + strand, within RR42_RS22120 at 303.139 kb on - strand, within RR42_RS22120 at 303.139 kb on - strand, within RR42_RS22120 at 303.177 kb on + strand, within RR42_RS22120 at 303.177 kb on + strand, within RR42_RS22120 at 303.177 kb on + strand, within RR42_RS22120 at 303.178 kb on - strand, within RR42_RS22120 at 303.300 kb on + strand, within RR42_RS22120 at 303.504 kb on - strand at 303.506 kb on + strand at 303.507 kb on - strand at 303.515 kb on + strand at 303.526 kb on + strand at 303.526 kb on + strand at 303.527 kb on - strand at 303.531 kb on + strand at 303.531 kb on + strand at 303.531 kb on + strand at 303.532 kb on - strand at 303.532 kb on - strand at 303.543 kb on + strand at 303.637 kb on - strand at 303.705 kb on + strand at 303.730 kb on + strand at 303.732 kb on + strand at 303.732 kb on + strand at 303.733 kb on - strand at 303.832 kb on + strand at 303.833 kb on - strand at 303.833 kb on - strand at 303.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 301,014 - RR42_RS22110 0.56 +0.3 301,176 - RR42_RS22110 0.65 -0.1 301,345 + RR42_RS22110 0.74 +1.5 301,346 - RR42_RS22110 0.74 +0.6 301,473 - RR42_RS22110 0.81 -0.4 301,736 + +0.3 301,897 + -0.3 302,029 + RR42_RS22115 0.20 +0.4 302,029 + RR42_RS22115 0.20 -0.4 302,029 + RR42_RS22115 0.20 -0.4 302,029 + RR42_RS22115 0.20 -0.7 302,029 + RR42_RS22115 0.20 -0.5 302,030 - RR42_RS22115 0.20 -1.2 302,030 - RR42_RS22115 0.20 +0.2 302,030 - RR42_RS22115 0.20 -0.6 302,030 - RR42_RS22115 0.20 +0.8 302,030 - RR42_RS22115 0.20 -2.0 302,097 - RR42_RS22115 0.26 -0.8 302,098 + RR42_RS22115 0.26 -0.2 302,098 + RR42_RS22115 0.26 +0.8 302,125 + RR42_RS22115 0.29 -0.3 302,236 + RR42_RS22115 0.40 +0.9 302,287 + RR42_RS22115 0.44 -0.7 302,288 - RR42_RS22115 0.45 -0.7 302,288 - RR42_RS22115 0.45 +0.4 302,395 - RR42_RS22115 0.55 -0.7 302,465 - RR42_RS22115 0.61 -1.1 302,539 + RR42_RS22115 0.68 -0.8 302,558 + RR42_RS22115 0.70 -1.4 302,653 + RR42_RS22115 0.79 -0.9 302,654 - RR42_RS22115 0.79 +0.2 302,663 - RR42_RS22115 0.80 -0.4 302,749 + RR42_RS22115 0.89 +0.4 302,896 + -0.2 302,896 + -0.0 302,897 - +0.7 302,901 + -0.8 302,975 + -1.1 302,975 + +0.6 302,976 - +0.7 302,978 - +0.5 303,095 + RR42_RS22120 0.33 -1.8 303,138 + RR42_RS22120 0.41 +0.3 303,139 - RR42_RS22120 0.42 -0.1 303,139 - RR42_RS22120 0.42 +0.3 303,177 + RR42_RS22120 0.49 -0.0 303,177 + RR42_RS22120 0.49 +0.2 303,177 + RR42_RS22120 0.49 +0.1 303,178 - RR42_RS22120 0.49 +0.1 303,300 + RR42_RS22120 0.73 +1.3 303,504 - -1.0 303,506 + +0.5 303,507 - +1.2 303,515 + +0.8 303,526 + -0.2 303,526 + +0.1 303,527 - -1.0 303,531 + +1.0 303,531 + +0.0 303,531 + -0.4 303,532 - -0.8 303,532 - -0.6 303,543 + +1.3 303,637 - +1.4 303,705 + +0.6 303,730 + +0.0 303,732 + +1.1 303,732 + +0.6 303,733 - +0.7 303,832 + -0.0 303,833 - +0.1 303,833 - +0.2 303,833 - -0.0
Or see this region's nucleotide sequence