Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21245 and RR42_RS21250 are separated by 247 nucleotides RR42_RS21250 and RR42_RS21255 are separated by 268 nucleotides RR42_RS21255 and RR42_RS21260 are separated by 118 nucleotides
RR42_RS21245: RR42_RS21245 - nicotinate-nucleotide pyrophosphorylase, at 92,417 to 93,253
_RS21245
RR42_RS21250: RR42_RS21250 - antibiotic biosynthesis monooxygenase, at 93,501 to 93,800
_RS21250
RR42_RS21255: RR42_RS21255 - LysR family transcriptional regulator, at 94,069 to 94,947
_RS21255
RR42_RS21260: RR42_RS21260 - isovaleryl-CoA dehydrogenase, at 95,066 to 96,862
_RS21260
Position (kb) 94
95 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 93.102 kb on - strand, within RR42_RS21245 at 93.102 kb on - strand, within RR42_RS21245 at 93.102 kb on - strand, within RR42_RS21245 at 93.102 kb on - strand, within RR42_RS21245 at 93.137 kb on - strand, within RR42_RS21245 at 93.138 kb on + strand, within RR42_RS21245 at 93.138 kb on + strand, within RR42_RS21245 at 93.138 kb on + strand, within RR42_RS21245 at 93.139 kb on - strand, within RR42_RS21245 at 93.139 kb on - strand, within RR42_RS21245 at 93.139 kb on - strand, within RR42_RS21245 at 93.249 kb on + strand at 93.250 kb on - strand at 93.250 kb on - strand at 93.250 kb on - strand at 93.324 kb on - strand at 93.350 kb on + strand at 93.396 kb on - strand at 93.431 kb on + strand at 93.431 kb on + strand at 93.431 kb on + strand at 93.431 kb on + strand at 93.431 kb on + strand at 93.432 kb on - strand at 93.432 kb on - strand at 93.432 kb on - strand at 93.891 kb on - strand at 93.925 kb on + strand at 93.926 kb on + strand at 93.969 kb on + strand at 93.970 kb on - strand at 94.142 kb on + strand at 94.142 kb on + strand at 94.143 kb on - strand at 94.143 kb on - strand at 94.143 kb on - strand at 94.143 kb on - strand at 94.301 kb on + strand, within RR42_RS21255 at 94.340 kb on + strand, within RR42_RS21255 at 94.340 kb on + strand, within RR42_RS21255 at 94.341 kb on - strand, within RR42_RS21255 at 94.344 kb on - strand, within RR42_RS21255 at 94.471 kb on + strand, within RR42_RS21255 at 94.640 kb on + strand, within RR42_RS21255 at 94.641 kb on - strand, within RR42_RS21255 at 94.842 kb on + strand, within RR42_RS21255 at 94.842 kb on + strand, within RR42_RS21255 at 94.925 kb on + strand at 94.962 kb on + strand at 95.010 kb on + strand at 95.013 kb on + strand at 95.013 kb on + strand at 95.013 kb on + strand at 95.014 kb on - strand at 95.027 kb on + strand at 95.060 kb on + strand at 95.061 kb on - strand at 95.061 kb on - strand at 95.198 kb on + strand at 95.198 kb on + strand at 95.199 kb on - strand at 95.287 kb on + strand, within RR42_RS21260 at 95.287 kb on + strand, within RR42_RS21260 at 95.369 kb on + strand, within RR42_RS21260 at 95.369 kb on + strand, within RR42_RS21260 at 95.369 kb on + strand, within RR42_RS21260 at 95.474 kb on + strand, within RR42_RS21260 at 95.475 kb on - strand, within RR42_RS21260 at 95.475 kb on - strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.630 kb on + strand, within RR42_RS21260 at 95.888 kb on + strand, within RR42_RS21260 at 95.888 kb on + strand, within RR42_RS21260 at 95.888 kb on + strand, within RR42_RS21260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 93,102 - RR42_RS21245 0.82 +0.7 93,102 - RR42_RS21245 0.82 +0.2 93,102 - RR42_RS21245 0.82 +0.0 93,102 - RR42_RS21245 0.82 -2.0 93,137 - RR42_RS21245 0.86 -0.2 93,138 + RR42_RS21245 0.86 -1.3 93,138 + RR42_RS21245 0.86 +2.4 93,138 + RR42_RS21245 0.86 -0.8 93,139 - RR42_RS21245 0.86 +1.2 93,139 - RR42_RS21245 0.86 +0.7 93,139 - RR42_RS21245 0.86 +1.2 93,249 + -0.2 93,250 - +0.8 93,250 - -0.0 93,250 - +0.8 93,324 - -0.3 93,350 + +0.9 93,396 - +0.4 93,431 + +0.7 93,431 + -1.6 93,431 + -3.0 93,431 + +0.6 93,431 + -0.5 93,432 - +0.1 93,432 - -0.5 93,432 - +0.1 93,891 - +0.7 93,925 + +0.6 93,926 + -0.8 93,969 + -0.2 93,970 - -1.3 94,142 + -1.2 94,142 + -2.1 94,143 - +0.1 94,143 - -0.7 94,143 - +0.2 94,143 - -0.7 94,301 + RR42_RS21255 0.26 +0.3 94,340 + RR42_RS21255 0.31 +0.2 94,340 + RR42_RS21255 0.31 +1.5 94,341 - RR42_RS21255 0.31 +0.4 94,344 - RR42_RS21255 0.31 -0.6 94,471 + RR42_RS21255 0.46 -0.3 94,640 + RR42_RS21255 0.65 +1.6 94,641 - RR42_RS21255 0.65 +0.0 94,842 + RR42_RS21255 0.88 -0.3 94,842 + RR42_RS21255 0.88 +1.4 94,925 + +0.5 94,962 + -0.1 95,010 + +0.2 95,013 + -3.2 95,013 + -0.7 95,013 + -0.2 95,014 - -0.8 95,027 + -0.1 95,060 + +1.9 95,061 - +0.8 95,061 - +0.9 95,198 + -2.1 95,198 + +0.4 95,199 - +0.4 95,287 + RR42_RS21260 0.12 +0.6 95,287 + RR42_RS21260 0.12 -0.1 95,369 + RR42_RS21260 0.17 -0.3 95,369 + RR42_RS21260 0.17 +0.3 95,369 + RR42_RS21260 0.17 +2.0 95,474 + RR42_RS21260 0.23 +0.2 95,475 - RR42_RS21260 0.23 -1.1 95,475 - RR42_RS21260 0.23 +0.5 95,486 + RR42_RS21260 0.23 +0.5 95,486 + RR42_RS21260 0.23 +0.9 95,486 + RR42_RS21260 0.23 -0.3 95,486 + RR42_RS21260 0.23 -1.8 95,486 + RR42_RS21260 0.23 +0.2 95,630 + RR42_RS21260 0.31 +0.9 95,888 + RR42_RS21260 0.46 +1.0 95,888 + RR42_RS21260 0.46 -0.3 95,888 + RR42_RS21260 0.46 -3.1
Or see this region's nucleotide sequence